BiomolecularInteractionsLeeds

Biomolecular Interactions technology facility at the University of Leeds.

SPR, ITC and MST for binding assays. Refeyn for solution molecular weights.

[University of Leeds @UniversityLeeds]

Since 2009, we’ve used this account to share University updates, highlight research stories, and connect with the Leeds community far and wide.

However, in recent years - like many others - we’ve experienced a huge drop in engagement due to developments on the platform.

[RadfordLab @RadfordLab]

Are you interested in proteins as exciting, dynamic interacting species. Come and join us!

Places are limited so please register ASAP at https://t.co/Fh05f7GKSt. (£25.00 registration only, including excellent catering).

[BiomolecularInteractionsLeeds @BindingLeeds]

Excited to have Neo detectors installed on our Fida this week. Extending the concentration range for size, shape, heterogeneity & aggregation measurements.

Kinetics & binding assays also from @FIDATechnology.
Thanks Tom & @JoanneWalter4
Many thanks @BBSRC for funding.

[Nature Communications @NatureComms]

@BobSchiffrin, @J_A_Crossley, @RadfordLab and colleagues based @AstburyCentre and @ScienceLeeds identify dual binding sites in the outer membrane protein chaperone SurA. #chaperones, #NMR, #smFRET

https://t.co/1aOaLGuIU3

[BiomolecularInteractionsLeeds @BindingLeeds]

@calfieri84 This is an idea from the old VP-ITC manual. In the syringe, CaCl2 5mM and in the cell, EDTA 0.4mM. Both in MES buffer 10mM pH 5.6.

For our ITC200, 1x 0.5ul injection then 19x 2ul with 2 minute spacing. Reference power set on 5 and temperature 25oC.

[BiomolecularInteractionsLeeds @BindingLeeds]

Mixed week of binding results, so it's good to finish the week with a positive, to check the machine is working properly - ITC of Ca2+/EDTA binding.

[BBS @BritBiophysSoc]

Abstract submission for the British Biophysical Society Biennial Meeting 2024 is now open!
https://t.co/ETRPclVuJP
Join us for three days of all things Biophysics - and for the first time in Wales! An exciting line-up of speakers, including this year's BBS awardees below:

[BiomolecularInteractionsLeeds @BindingLeeds]

I'm proud of how the facility contributes to some UG and Masters practicals. Very pleased that someone nominated me for one of the Faculty partnership awards - surprised to actually get the award.

Couldn't have done it without our facility and teaching lab technical staff.

[BiomolecularInteractionsLeeds @BindingLeeds]

This is nice - hydrodynamic radius and molecular weight conversion. Especially useful when interpreting Fida measurements @FIDATechnology

Well, "apparent" M.Wt. - think about folding, shape and multimerisation too if the numbers don't match.
https://t.co/QWQDFRqGlX

[BiomolecularInteractionsLeeds @BindingLeeds]

Super sessions at the @FIDATechnology user meeting in Copenhagen, seeing how Fida fits with the biophysics & structural biology tool-kit. Designing better assays workshops.

Great talk from @aip_taylor, @RadfordLab, using Fida & other tech to study protein aggregation pathways.

[BiomolecularInteractionsLeeds @BindingLeeds]

Really pleased the facility could contribute lots of ITC binding results to this publication.

Congratulations on your first group publication @JamesFRoss2.

[James F Ross @JamesFRoss2]

Interested in the mutational plasticity of membrane traffickers like cholera toxin b-subunit?? Check out the first ever paper from the RossLab here: https://t.co/2VLbMnzbIa
with support from @EPSRC , @wbturnbull , @BindingLeeds , @Toxins_MDPI

[BiomolecularInteractionsLeeds @BindingLeeds]

@klausenhauser aah, yes, set to 5, 5 point zero 😉

[BiomolecularInteractionsLeeds @BindingLeeds]

Lots of good ITC binding results in recent weeks.

Disproportionately pleased with this though...
#equilibrated
#clean
#ITC

[BiomolecularInteractionsLeeds @BindingLeeds]

Loving the rapid size analysis from Fida 1, before an assay or troubleshooting issues after. A gallery of results.

Good - large signal, low aggregation
Not great - less signal than expected
Oops - aggregation, low signal

Thank you for the loan @FIDATechnology
@RadfordLab

[BiomolecularInteractionsLeeds @BindingLeeds]

You're gonna need a bigger rack.

122 SPR cycles for small-molecule titrations - 10+ hours run time.
Successful week of binding assays using #Biacore

[Fidabio @FIDATechnology]

Dear users, it is time to sing up for Fidabio User Group meeting 2024! Few last speaking slots remain available, so if you want to make sure your research is seen, hurry up:
https://t.co/AoiW3025jN

[BiomolecularInteractionsLeeds @BindingLeeds]

Installation and training on our new Dianthus from @NanoTemper

Binding assays especially for tricky systems - membrane proteins, multi-molecular complexes and small molecule libraries.

Many thanks @The_MRC for funding.
@AstburyCentre @ScienceLeeds
#hugthebox

[BiomolecularInteractionsLeeds @BindingLeeds]

Solution measurements of size & shape.

Refeyn mass photometry of M.Wt. - VLPs & proteins (left-hand pics). @refeynit

Loving Fida also for smaller & unstructured molecules, aggregation assays, Ex488 & 280nm, automation...
V. grateful to @FIDATechnology for the loan.
@RadfordLab

[BiomolecularInteractionsLeeds @BindingLeeds]

Biomolecular interactions - our facility technologies.

SPR, ITC and MST for affinity, kinetics, thermodynamics and stoichiometry info.

Watch this space...
#Dianthus
@NanoTemper

[BiomolecularInteractionsLeeds @BindingLeeds]

My last practical session with our Biopharma students today. Including mAb purification & SDS-PAGE.

Have you purified a good (enough) reagent?
Is one buffer better than another?
Enough capacity in 1 column?
+ & - DTT (2 images of each)

Thank you students - it's been good.