Dewar Lab

We are the Dewar lab at Vanderbilt University. We study the biochemistry of DNA replication using in vitro cell-free approaches. #GelsNotCells #SometimesCells

[Dewar Lab @Dewar_Lab]

Our first foray into replication fork processing is now available! 🐸🧍🧬🧪 Key points:
- Uncoupling causes degradation and reversal
- Degradation occurs before and after reversal
- Replisomes are retained throughout
See model for how we think this works🙏
https://t.co/gxJhA1ag6G

[Evi Soutoglou @EviSoutoglou]

Happy to share our study about the role of Top2 complexes in different chromatin contexts!
We find that Top2 catalytic inhibition leads exclusively to instability of the repetitive genome which depends on Top2a. For more details of how, please read:
https://t.co/s14vO6YSkB

[Karlene Cimprich @KarleneCimprich]

The Cimprich lab has research assistant positions available.  If you (or someone you know) might be interested please apply here: 

https://t.co/Ol2CHE5GC3

https://t.co/ccXSUEnbHF

[Dewar Lab @Dewar_Lab]

We're looking for a new research technician to join our lab! Reasons to join:
1. Great science 🐸🧬
2. Fun & supportive environment 😃🙌
3. Excellent training record 🥇🎉
https://t.co/dvJPBusQYL
Please share with any class of '23 graduates who might be interested!

[Bill Dynan - NAR Cancer Editor @NAR_Cancer_EIC]

AP endonuclease 2 (APE2) as an emerging therapeutic target– More from our special collection on DNA repair and cancer therapeutics. From @ShanYanlab @UNCCBiology https://t.co/W4n0eIigh8

[Dewar Lab @Dewar_Lab]

Our latest termination paper is online! 🐸🧬➡️⬅️🧬Key points:
- RTEL1-MCM10 is a 'backup' mechanism for when TOP2 is compromised
- MCM10 promotes RTEL1 binding/function
- RTEL1-MCM10 don't regulate topology but do act as a general stalled fork response
https://t.co/apUTnQ1oX8

[James Dewar @JamesMDewar]

Please forward this to any senior graduate students you know. Should be lots of fun! I can't wait to meet the participating trainees and hear about their research!

[James Dewar @JamesMDewar]

🧬🛠️ We had an amazing time at the Mammalian DNA Repair GRC in Ventura, CA. Fantastic presentations and discussions at a great location. 🙏 to @Opreskolab and @RogerRogergr for organizing. Can't wait for the next one! @GordonConf #DNARepair

[Vanderbilt School of Medicine Basic Sciences @VUBasicSciences]

Researchers from the @Topo_Lab were instrumental in the development of the first new antibiotic treatment for uncomplicated urinary tract infections in more than 20 years.

Their collaborators @GSK will soon be filing for FDA approval.

https://t.co/iuagwmYv51

[The Hiebert Lab @HiebertLab]

Check out our latest story in @MolecularCell. Our team pairs the dTAG system with deep genomic analysis to determine the spatiotemporal role of SMARCA5 in the maintenance of chromatin structure and function.
https://t.co/i1Nxulgxa0

[andre nussenzweig lab @nussenza]

Postdoctoral positions available in Nussenzweig lab to study mechanisms involved in DNA repair- 1st position (wet lab) focuses on using cutting-edge cellular, molecular, and genomic tools. 2nd uses computational approaches to study DNA repair.

[hopfnerlab @hopfnerlab]

Our new cryo-EM structure explains how the MRN complex can act both as DNA double-strand break sensor & DNA tethering device, read the paper here:
https://t.co/XhGnEP18Dy

Huge thanks to all collaboration partners W.Roos, B.Steigenberger and C. Junge!

#cryoEM, #DNArepair, #MRN

[Tata Kavlashvili @TKavlashvili]

Wish my labmates had warned me.. would have cleaned up my bench. #PhDone

[David Cortez @davidkcortez]

The Department of Biochemistry @VandyBiochem in Vanderbilt Basic Sciences @VUBasicSciences is recruiting new faculty! Apply here: https://t.co/ahdlMl9iEm

[🧬 The Coster Lab 🔬 @CosterLab]

📢 Hot off the press 📢

🧬 Ever wonder why some DNA sequences are difficult to replicate?

Using reconstituted replisomes we revealed that structure-forming repeats induce robust replication stalling and uncovered structure-specific recovery mechanisms:
https://t.co/BajNVcpT9w

[Dewar Lab @Dewar_Lab]

Excited to share our latest paper, just published in EMBO J: https://t.co/BBmyvJiBwO
We found that topoisomerase II poisons exert different and unexpected effects on DNA replication. Congrats @sabrinadoessci1! Thanks also to our collaborators @Topo_Lab and @davidkcortez

[Tata Kavlashvili @TKavlashvili]

Really excited to share the methods chapter we wrote for DNA Damage Responses as a part of Methods in Molecular Biology series. Here we describe really fun assays to monitor DNA replication termination using 🐸🥚 extracts! #gelsnotcells ⁦@Dewar_Lab⁩ https://t.co/OzNskAwLeu

[Dewar Lab @Dewar_Lab]

Thrilled to announce another preprint from our lab! @LillianVCampos found that RTEL1 and MCM10 help replication forks overcome topological stress during termination. Thanks also to @BFEichman @DarrenHeintzman @TKavlashvili

[bioRxiv @biorxivpreprint]

RTEL1 and MCM10 overcome topological stress during vertebrate replication termination https://t.co/ADt8we8tGU #bioRxiv

[Tom Deegan @TDDeego]

Not long left to apply for postdoc positions on 🧬 replication in my new lab @mrc_hgu @EdinUni_IGC. A range of projects available, some of them related to https://t.co/xf1FpiZKus. Informal enquiries welcome.

https://t.co/p1qAE8bvO3

[Dewar Lab @Dewar_Lab]

A great opportunity for anyone looking for an exciting post-doctoral position!

[Sharon B. Cantor @CantorLab]

Postdoc positions! Come join us and address key biological questions about how Replication Gaps impact cancer, therapy response and developmental disease while learning the latest genome analysis techniques.
Interested, [email protected]