Luis Wong-Dilworth

Doctoral candidate in the Bottanelli Lab @Fran_Bottanelli @FUBiochemistry

[Ishier Raote @ishier_raote]

Terrific preprint from @Fran_Bottanelli! ER-to-Golgi transport via an ARF4 tubulo-vesicular network connecting ER and Golgi-associated pre-cisternae
Nanoscale imaging reveals the mechanisms of ER-to-Golgi transport via a dynamic tubular-vesicular network https://t.co/LX2jWMaBXa

[Luis Wong-Dilworth @FreeThePipette]

More tubular networks also in plants!

[Boutté Yohann @YohannBoutte]

Here it is ! https://t.co/b5JUECUTeY
The potency of super-resolution microscopy unveiled a tubular network as a trafficking intermediate between the ER and the Golgi in plants! Similar observations in animal cells by @Fran_Bottanelli suggest an evolutionary conserved mechanism.

[Francesca Bottanelli @Fran_Bottanelli]

3 first authors! 2 papers! Looking forward to getting feedback from the community! @A_STEDhammer @petiaadarska @FreeThePipette

[Luis Wong-Dilworth @FreeThePipette]

Tubules are the new headliners today!

[Francesca Bottanelli @Fran_Bottanelli]

Wondering how cargo is shuttled around between compartments in the cell? We think it is via dynamic tubular vesicular compartments! Check out our two new pre-prints https://t.co/O0pq0NGsn6 and https://t.co/PHb97FFuCc

[Luis Wong-Dilworth @FreeThePipette]

Your TubER driver has arrived.
Check out the latest insights into ER-to-Golgi traffic from the Bottanelli Lab! @Fran_Bottanelli
https://t.co/DTIEwlSHv8

[Francesca Bottanelli @Fran_Bottanelli]

Paper accepted celebrations! 🤩 good job @FreeThePipette and all co authors!

[Francesca Bottanelli @Fran_Bottanelli]

A tour the force of #geneediting and #STED. We have followed up on some of these findings and have 2 papers that will drop soon! looking at endogenous proteins at high resolution in their own unperturbed cellular environment is helping to reveal important trafficking mechanisms!

[Journal of Cell Biology @JCellBiol]

Luis Wong-Dilworth @freethepipette, Francesca Bottanelli @fran_bottanelli et al. use STED super-resolution #microscopy to reveal the nanoscale localization of ARF #GTPases, suggesting distinct functions for ARF paralogs in intracellular #trafficking. https://t.co/2rwNuGu0Iz

[Journal of Cell Biology @JCellBiol]

STED imaging of endogenously tagged ARF #GTPases reveals their distinct nanoscale localizations, say Luis Wong-Dilworth @freethepipette, Francesca Bottanelli @fran_bottanelli and colleagues
#microscopy

[Luis Wong-Dilworth @FreeThePipette]

Cracking the ARF GTPases code. Thank you for the great opportunity @Fran_Bottanelli and to all members from the lab that made this possible. Excited to learn the insights that follow from this work!

[Journal of Cell Biology @JCellBiol]

.@freethepipette, @fran_bottanelli and colleagues combine gene editing and STED super-resolution #microscopy to reveal the nanoscale localization of ARF #GTPases in living cells https://t.co/2rwNuGu0Iz

[Luis Wong-Dilworth @FreeThePipette]

@ddierks91 @Fran_Bottanelli Thank you Dave! :)

[Francesca Bottanelli @Fran_Bottanelli]

Tune in this Thursday when I will talk about our work on #geneediting and live-cell #STED to understand the function of #ARF #GTPases in living cells! Pre-print https://t.co/n3GlFo0lSB

[Francesca Bottanelli @Fran_Bottanelli]

And as promised here is a spontaneous Twittorial about our latest preprint. I have always been fascinated by ARF GTPases, they are really masters of the cells regulating #membrane homeostasis, #metabolism, #cytoskeleton. Humans have 5 ARFs and WHY DO WE NEED SO MANY?

[Francesca Bottanelli @Fran_Bottanelli]

Very excited to have this out! Here we use #CRISPR and #STED #superres microscopy to map the nanoscale localization of ARF GTPases! With many surprises! Twittorial this evening 😊

[Francesca Bottanelli @Fran_Bottanelli]

Manuscript uploaded to BioRxiv 😎 Awesome gene editing and #STED tour de force by @FreeThePipette and many others!

[Francesca Bottanelli @Fran_Bottanelli]

Check out our short review about #ARF #GTPases. ARFs are master regulators of trafficking and we still have so much to discover about their cellular functions! We also share some of our views and opinions. Great work by two PhD students @FreeThePipette and @petiaadarska!

[American Society for Cell Biology @ASCBiology]

Interested in exciting new #membrane trafficking research and discussions? There's a Slack for that! Organized by Fran Bottanelli (FU Berlin), Ishier Raote (CRG, Barcelona) and Felix Campelo (ICFO, Barcelona). Join at: https://t.co/a9iXjgxMiE