@McMurraylab In S288c - the reference genome - HMRA has a "stuck" mutatiion that has reduced HO cutting when at MAT. and when MATa-stk switches to MATalpha-stk it is sterile (MATalpha1-minus) and even harder to switch.
@McMurraylab For what it's worth, HMRa in the reference genome is a "stuck" allele that greatly reduces HO cleavage. Moreover, when this same mutation at MATa-stk is switched to MATalpha-stk it is sterile (alpha 1-minus) and even more difficult to switch.
@McMurraylab good question. The assumption might be wrong - that in the wild intra-ascal matings are the norm; but it seems logical, and especially for heterothallic yeasts. I also don't know what fraction of wildtype yeast are homothallic. more below
In antigenic variation in Lyme disease the vlsE surface protein gene is repeatedly changed by recombination with 15 adjacent pseudogenes. We tried to stimulate this in culture by cutting with Cas9. Mais non! Breaks cause loss of the immunity plasmid. https://t.co/7jMs6PFnZC
The 52nd Rosenstiel Award goes to two excepetional women: Erin Schuman and Christine Holt for their discoveries of the mechanisms of local protein translation that enables a neuron to get from the eye to the brain. https://t.co/dkkXmIswxy
So suddenly I find myself looking for a new postdoc to work on double-strand break repair in yeast: mutations arising during gene editing and other types of DNA repair. Applicants should send a CV and cover letter to Jim Haber ([email protected]).
Please retweet!
So suddenly I find myself looking for a new postdoc to work on double-strand break repair in yeast: mutations arising during gene editing and other types of DNA repair. Applicants should send a CV and cover letter to Jim Haber ([email protected]).
Please retweet!
An outrageous > 6-month delay by the USCIS in approving a waiver for my postdoc to remain in the US has led to her departure from my lab – she was not allowed to work or draw a salary while the wheels of bureaucracy ground on. What a mess! So now...
The Department of Biology at Brandeis University, Waltham, MA (Boston) seeks an instructor for the course BIOL 128, Human Genetics, in the Fall 2022 semester.
https://t.co/K9LMLVArmx
@geneticssociety The Department of Biology at Brandeis University, Waltham, MA (Boston) seeks an instructor for the course BIOL 128, Human Genetics, in the Fall 2022 semester.
https://t.co/K9LMLVArmx