HeilemannLab (👉🏻 heilemannlab.bsky.social)

Single-molecule biophysics lab at Goethe University Frankfurt. Super-resolution microscopy.
We moved 👉🏻

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

Two great days of science at the #SMLMS2022, culminating with a conference dinner on the boat on the Seine. So many great conversations 🙏. Happy to present our choreography to show our appreciation to this event (watch the light pattern!).

[Beck Laboratory @Beck_Laboratory]

… and perfect timing. Work by @HuaipengXing with @FrydmanLab out @Nature. PDCD5 binds virtually all open #TRiC, while closed TRiC forms clusters inside of cells. Congrats to all authors. https://t.co/5OX60e04zp

[Dr. Florencia Sánchez @MFSanchez_Lab]

Open postdoctoral position in my group! https://t.co/iTqccAquiU We search for a very talented student in the field of super resolution microscopy! @UK_Muenster @uni_muenster #academia #newPI

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

Kudos to Enya & Laurell for their fantastic work! Great collab🙏w/ Bernd Rieger & Sjoerd Stallinga (TU Delft) Michaela MüllerMcNicoll (@goetheuni) & great support by @ashwin0512. We are very excited about the results & curious to see your feedback 👉🏻 https://t.co/T6LykcSli3

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

We modified our STED🔬to make it more efficient ('smart') to acquire high-resolution data of small and rare objects in a 3D cell volume. This enabled collecting a large amount of data in a fully automated fashion. With that, we visualized the ultra-structure a nuclear body👇🏻(1/3)

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

By labeling the architectural RNA NEAT1 in paraspeckles (PS) at various positions using RNA-FISH-DNA-LNA probes, we learnt 3 new things: (1) the morphological space & shape progression of PS, (2) the orientation/packing of its RNA, (3) the coarse conformation of NEAT1. (2/3)

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

https://t.co/ZEnPwl692x
🙏hoping to re-connect to many of you

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

Long-term single-protein tracking up to 30 min allowed monitoring the dynamics of EGFR activation in living cells (and more)👇🏻Great work by a fantastic team and great collaboration with @JulianKompa Kai Johnsson @mpi_mr_hd 🙏 @sfb1507 @goetheuni

[Angewandte Chemie @angew_chem]

Long-Term Single-Molecule Tracking in Living Cells using Weak-Affinity Protein Labeling (Mike Heilemann and co-workers) @HeilemannLab #openaccess https://t.co/YMpzZHuxLI

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

@sjoerdjlvanwijk @CovinoLab @goetheuni @fias_science @sfb1507 Thank you, Sjoerd! 🙏

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

🙏happy that this article is out now: we used an integrative approach (MD, single-molecule imaging, FRET) to reveal early activation of the receptor tyrosine kinase MET in cells👇🏻A wonderful collaboration with @CovinoLab & great co-workers 🙏@goetheuni @fias_science @sfb1507

[Roberto Covino @CovinoLab]

Check out how we used simulations and single molecule FRET to determine how a invasion bacterial protein activates a plasma membrane receptor to enter our cells. Kudos to @serena_arghittu and @gabriel_hella, and great collaboration with @HeilemannLab

https://t.co/891amj93fH

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

Many thanks to the reviewers for great input (yes) and to the Editor & team of @NatureComms 🙏

[Nano Bio Optics Lab (Yoav Shechtman) @ShechtmanLab]

Gen-AI producing fancy data augmentation for Super-resolution microscopy! Great work @AlonSaguy and team! @HenriquesLab @HeilemannLab

[Zhixing Chen @ZhixingChen2]

Our Gentle Rhodamines is finally published @ACSCentSci.
See the healthy cytoskeleton free from photodamage even after 800 frames! 🦾💪
https://t.co/aN7gNl1iaV
Great collaboration with Julian Kompa, the Johnsson group, and Spirochrome! @JulianKompa @spirochrome @TianyanL

[Achillefs Kapanidis @AchillesKap]

3 weeks to go... send us your single-molecule bacterial stories for our Cotswolds get-together!

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

@glembockyte @mpi_mr_hd Congratulations, Victorija! And a great start! 🎉

[Siewert Hugelier @SHugelier]

Interested in a fully automatic analysis pipeline to quantify and classify your 2D and 3D Single-Molecule Localization Microscopy (SMLM) data in an unbiased way? Have a look at our latest work covering ECLiPSE, developed in the @Melike_Lak lab!
https://t.co/2lXkhvOZhJ 1/11

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

@DedeckerLab @naturemethods @vandenberglab Congratulations, Peter & team, very nice work!

[Julien Hiblot @JulienHiblot]

Why reinvent the wheel when you can enhance it? We’ve focused on making SNAP-tag better. @SteffiKuehn developed SNAP-tag2, with an optimized substrate. Faster labeling, brighter fluorescence-this is the upgrade you've been waiting for!

[Single Molecule Localization Microscopy Symposium @SMLM_Symposium]

We see each other next year in Bonn, Germany in #SMLMS2025 hosted by @UEndesfelder and @HeilemannLab

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

@Dr_Fluo @Melike_Lak ... very good memories. I am waiting for the group photo of #SMLMS2024 to put that next to it in the gallery 😅

[HeilemannLab (👉🏻 heilemannlab.bsky.social) @HeilemannLab]

I am chiming in: fantastic meeting, congratulations and thank you to @HenriquesLab and @MicrobeMatos & entire team! #SMLMS2024 @SMLM_Symposium
Looking forward to #SMLMS2025 and working with @UEndesfelder to make it happen. The bar is high 😅. Hope to see you all again in Bonn!