Joey Tidei

PhD candidate in the Beach (@myosincity) and Oakes (@pwoakes) Labs at Loyola University Chicago. Badger Alum. Former beer nerd. I like neurons. I like bread.

[Joey Tidei @J__Today]

CAD cells reign supreme! Congrats @chillinwithpfn1 and @CisternaBA!!

[Journal of Cell Biology @JCellBiol]

Our October issue is out! https://t.co/l8Eiamr5Kp The cover shows a color-coded projection through time of confocal images of a mouse primary Th1 T cell expressing EGFP-Lifeact migrating in confinement. From @AlexiaCaillier, @pwoakes et al. (https://t.co/dN8J8i209s)

[Joey Tidei @J__Today]

@DaniBeckman @fluoroforce Thank you!

[Joey Tidei @J__Today]

Very fortunate to have access to an abundance of scopes to capture images like this. These are primary neurons treated with blebbistatin and stained for acetylated tubulin. @fluoroforce iLUT again coming in HOT this #FluorescenceFriday

[Joey Tidei @J__Today]

@Roy_Lab_Thinks Could there be actin filaments coupled to these microtubules? I've never seen NM2 bound to MTs like this but you can see the motor head doublets. Cool image!

[Caillier Alexia @AlexiaCaillier]

Really proud to see this paper finally out ! 🤩🎉🍾 Thank you to @JCellBiol and the reviewers for their precious comments that definitely strengthened our work.

See our previous thread for most of the details ! https://t.co/hq7nYm36FR

[Joey Tidei @J__Today]

@Kel_Nem A human baby indeed! Thank you!

[Joey Tidei @J__Today]

Life has been pulling me away from the scope (I had a baby named Henry!), but #FlourescenceFriday never takes a break. CAD cell with a dual tag myosin 2 construct - green on the motor heads and magenta on the tails, giving a🟢🟣🟢triplet. Can even see a few processive runs🏃‍♂️

[Joey Tidei @J__Today]

@jenkins_paul Congrats!!!

[Pirta Hotulainen @PirtaHotulainen]

Our actin in AIS plasticity story, led by PhD student David Micinski, is now online in Frontiers in Molecular Neuroscience. We like to thank our excellent reviewers whose comments and requests clearly improved the paper.
https://t.co/ctwK2QUaXj
Thread below👇

[Journal of Cell Biology @JCellBiol]

Our April cover ➡️ https://t.co/skXux0dgDv shows overlayed temporal projection of actomyosin dynamics in the leading edge of migrating mouse embryonic fibroblast expressing EGFP-non-muscle myosin & mScarlet F-Tractin. From @fluoroforce, @myosincity et al. https://t.co/vtQid8JXZW

[Felipe Opazo @FelipeOpaz_o]

Very happy to see the central Ph.D. topic (out of several) of @N_Mougios in @biorxivpreprint. He established a universal way to make all your antibody-based assays easily multiplexable by using erasable secondary #nanobodies. See it here: https://t.co/REkFLbycxu

[Joey Tidei @J__Today]

Actin retrograde flow in the lamellapodia of a stage one neuron. Happy #FluorescenceFriday !

[Joey Tidei @J__Today]

@AnhHLe2702 This was more or less me with an extra well of neurons trying a random antibody from our stock to explore, but this is great info to know. Thank you!

[Joey Tidei @J__Today]

Primary rat hippocampal neurons at DIV14 stained for Active-RhoA🔵and Phospho-Myosin Light Chain🟣. No AI required for this #FluorescenceFriday .

[Joey Tidei @J__Today]

@pablo_jack Yep, anti-RhoA-GTP from NewEast

[Joey Tidei @J__Today]

@AnhHLe2702 Thank you! This was actually the first attempt at using the antibody so I don't have a great answer for you, but the signal and localization was quite dispersed along neurites and cortical at the soma. I can certainly get back to you with a better answer soon!

[Joey Tidei @J__Today]

@Roy_Lab_Thinks @jenkins_paul

[Journal of Cell Biology @JCellBiol]

Quintanilla (@fluoroforce), Oakes (@pwoakes), Beach (@myosincity) and colleagues @LoyolaChicago demonstrate subcellular biophysical mechanisms that enable myosin 2 filament assembly. https://t.co/VQqK2bHjwC

@MatthewAkamatsu @Coronin @taraskalab
#Biophysics #Cytoskeleton

[Joey Tidei @J__Today]

@JaroslavaNKA