Bloom Lab

Lab studying evolution of proteins and viruses. Affiliated with @fredhutch @HHMINEWS @uwgenome.

Opinions are my own and do not reflect those of my employer.

[Bloom Lab @jbloom_lab]

Please see the preprint for additional details: https://t.co/5CEN4pfvYd
Thanks to Bernadeta Dadonaite for leading study, and @anniedosey, @Jenny_Ahn0, @timcyuu, Sara Sunshine, Ariana Farrell, and @KingLabIPD for valuable contributions.

[Bloom Lab @jbloom_lab]

In new study led by Bernadeta Dadonaite, we show many influenza HAs (H5, H7, H9, H1, H2, H3) can use avian or human MHC-II to enter cells.

We then use novel combo of deep mutational scanning & cryoEM to define how H5 HA binds tufted duck MHC-II

Preprint: https://t.co/5CEN4pfvYd

[Bloom Lab @jbloom_lab]

Note our study used pseudoviruses and conditionally replicative virions to ensure biosafety, and reports deep mutational scanning only for HA usage of tufted duck MHC-II to limit any information hazard concerns.

[Bloom Lab @jbloom_lab]

Here are the slides I am presenting at this morning's @CEIRRNetwork seminar related to characterizing the human neutralizing antibody landscape to human seasonal influenza for purposes such as vaccine strain selection:

https://t.co/nSlk3QGGnY

[Bloom Lab @jbloom_lab]

See the full paper for additional details and analyses:
https://t.co/NSWtPLR8u7

Thanks to @bblarsen1 for leading study & our collaborators in @dveesler lab.

[Bloom Lab @jbloom_lab]

In new study led by @bblarsen1, we map functional constraint across the Nipah virus F protein to define constrained epitopes for antibody targeting and identify mutations that stabilize the prefusion conformation for vaccine immunogens

https://t.co/NSWtPLR8u7

[Bloom Lab @jbloom_lab]

We also defined how F mutations affect neutralization by a panel of monoclonal antibodies. This allowed us to quantify the resilience of different antibodies to escape, and predict which antibodies also neutralize the related Hendra virus.