Lab studying evolution of proteins and viruses. Affiliated with @fredhutch @HHMINEWS @uwgenome.
Opinions are my own and do not reflect those of my employer.
Here are the slides I am presenting at this morning's @CEIRRNetwork seminar related to characterizing the human neutralizing antibody landscape to human seasonal influenza for purposes such as vaccine strain selection:
https://t.co/nSlk3QGGnY
See the full paper for additional details and analyses:
https://t.co/NSWtPLR8u7
Thanks to @bblarsen1 for leading study & our collaborators in @dveesler lab.
In new study led by @bblarsen1, we map functional constraint across the Nipah virus F protein to define constrained epitopes for antibody targeting and identify mutations that stabilize the prefusion conformation for vaccine immunogens
https://t.co/NSWtPLR8u7
We also defined how F mutations affect neutralization by a panel of monoclonal antibodies. This allowed us to quantify the resilience of different antibodies to escape, and predict which antibodies also neutralize the related Hendra virus.
In new work in @jbloom_lab , we asked: how does endemic coronavirus keep evolving to erode human antibodies without disrupting spike function?
https://t.co/uCDd2dJcSC
@timcyuu@CKikawa The final version of this article has been published in @NatureEcoEvo alongside a nice News and Views by Seth Zost: https://t.co/baPT89JPy8
We found extensive epistatic entrenchment w respect to cell entry in receptor binding pocket (see below), consistent w prior work by @wchnicholas@RobertPdeVries1
https://t.co/xxX8ZJV192
https://t.co/BIQWt7ND9w
But minimal epistatic entrenchment w respect to HA stability.
All the data are available for visualization and download at https://t.co/O6vIiI2Xnd
Thanks to @timcyuu for leading study, and also @CKikawa, Bernadeta Dadonaite, Andrea Loes, & Jane Englund.
In study led by @CKikawa & Andrea Loes, we use new assay to measure ~10,000 neutralization titers to recent influenza strains & show titers correlate w evolutionary success of viral strains
Similar data could help forecast evolution for vaccine selection
https://t.co/OxwiNnXUuU
Finally, from biosafety perspective, we studied only viruses with HAs from seasonal influenza strains currently or recently circulating in humans. Our data therefore provides real-time portrait of immunity to strains already in current human population.
We have posted data providing real-time measurement of human neutralizing antibody landscape to seasonal influenza
Data explain spread of subclades K (H3N2) & D.3.1.1 (H1N1), identify subclade K subvariants w reduced neutralization & can inform choice of strains for next vaccine