Bloom Lab

Lab studying evolution of proteins and viruses. Affiliated with @fredhutch @HHMINEWS @uwgenome.

Opinions are my own and do not reflect those of my employer.

[Bloom Lab @jbloom_lab]

Here are the slides I am presenting at this morning's @CEIRRNetwork seminar related to characterizing the human neutralizing antibody landscape to human seasonal influenza for purposes such as vaccine strain selection:

https://t.co/nSlk3QGGnY

[Bloom Lab @jbloom_lab]

See the full paper for additional details and analyses:
https://t.co/NSWtPLR8u7

Thanks to @bblarsen1 for leading study & our collaborators in @dveesler lab.

[Bloom Lab @jbloom_lab]

In new study led by @bblarsen1, we map functional constraint across the Nipah virus F protein to define constrained epitopes for antibody targeting and identify mutations that stabilize the prefusion conformation for vaccine immunogens

https://t.co/NSWtPLR8u7

[Bloom Lab @jbloom_lab]

We also defined how F mutations affect neutralization by a panel of monoclonal antibodies. This allowed us to quantify the resilience of different antibodies to escape, and predict which antibodies also neutralize the related Hendra virus.

[Sheri Harari @SheriHarari]

In new work in @jbloom_lab , we asked: how does endemic coronavirus keep evolving to erode human antibodies without disrupting spike function?
https://t.co/uCDd2dJcSC

[Bloom Lab @jbloom_lab]

@timcyuu @CKikawa The final version of this article has been published in @NatureEcoEvo alongside a nice News and Views by Seth Zost: https://t.co/baPT89JPy8

[Bloom Lab @jbloom_lab]

We found extensive epistatic entrenchment w respect to cell entry in receptor binding pocket (see below), consistent w prior work by @wchnicholas @RobertPdeVries1
https://t.co/xxX8ZJV192
https://t.co/BIQWt7ND9w

But minimal epistatic entrenchment w respect to HA stability.

[Bloom Lab @jbloom_lab]

All the data are available for visualization and download at https://t.co/O6vIiI2Xnd

Thanks to @timcyuu for leading study, and also @CKikawa, Bernadeta Dadonaite, Andrea Loes, & Jane Englund.

[Bloom Lab @jbloom_lab]

@CKikawa @huddlej @marlinfiggins @Miriampippaart @ETroisi10 @SCOTTeHENSLEY @trvrb The final version of record of this study has been published in @eLife alongside a nice insight perspective: https://t.co/iptxQZJE5m

[Bloom Lab @jbloom_lab]

In study led by @CKikawa & Andrea Loes, we use new assay to measure ~10,000 neutralization titers to recent influenza strains & show titers correlate w evolutionary success of viral strains

Similar data could help forecast evolution for vaccine selection

https://t.co/OxwiNnXUuU

[Bloom Lab @jbloom_lab]

All data described above are at https://t.co/LrHqo6lLz0

Thanks to @CKikawa & Andrea Loes for leading study, w important contributions from @huddlej, @marlinfiggins, @Miriampippaart, Tachianna Griffiths, @ETroisi10, Heidi Peck, Ian Barr, Jan Englund, @SCOTTeHENSLEY, @trvrb

[Bloom Lab @jbloom_lab]

Finally, from biosafety perspective, we studied only viruses with HAs from seasonal influenza strains currently or recently circulating in humans. Our data therefore provides real-time portrait of immunity to strains already in current human population.

[Bloom Lab @jbloom_lab]

We have posted data providing real-time measurement of human neutralizing antibody landscape to seasonal influenza

Data explain spread of subclades K (H3N2) & D.3.1.1 (H1N1), identify subclade K subvariants w reduced neutralization & can inform choice of strains for next vaccine

[Bloom Lab @jbloom_lab]

Also, thanks to @CEIRRNetwork @NIAIDNews @hhmi_science for support.