In new study led by @timcyuu, we measure how mutations to H3 flu HA affect cell entry, stability & antibody escape
We find pleiotropic effects of mutations on these phenotypes shape evolution: epistasis alleviates cell-entry but not stability constraints
https://t.co/mZs4KdCvkv
In study led by @CKikawa & Andrea Loes, we use new assay to measure ~10,000 neutralization titers to recent influenza strains & show titers correlate w evolutionary success of viral strains
Similar data could help forecast evolution for vaccine selection
https://t.co/OxwiNnXUuU
How do enhancers work over distances that sometimes exceed megabases? Excited to share our work led by @gracecbower where we uncover a unique sequence signature globally associated with long-range enhancer-promoter interactions in developing limb buds: https://t.co/n0p7BdxSdq 1/
In new study led by @bdadonaite, we measure how all mutations to H5 influenza HA affect four molecular phenotypes relevant to pandemic risk:
https://t.co/QOjVTF4h9L
Results can inform surveillance of ongoing evolution of H5N1.
In new study led by @bblarsen1 in collab w @veeslerlab @VUMC_Vaccines we map functional & antigenic landscape of Nipah virus receptor binding protein (RBP)
https://t.co/EyFWcxzRe9
Results elucidate constraints on RBP function & provide insight re protein’s evolutionary potential
In new study led by Andrea Loes, we develop sequencing-based neutralization assay that measures replicate curves for hundreds of virus-serum pairs per 96-well plate (see image below)
https://t.co/hCkjUEiRpV
We apply assay to characterize response to repeat influenza vaccination
In new study led by Caleb Carr & @khdcrawford, we measure how all mutation to Lassa virus glycoprotein complex (GPC) affect cell entry & antibody escape
Results show how prospective assessment of effects of mutations can inform design of countermeasures
https://t.co/M2LmbTbwKm
In new study led by Frances Welsh, we map how mutations to influenza affect neutralization by antibodies from humans of various ages
We find differences in mutation effects among age groups
Virus has evolved especially to escape antibodies of teenagers
https://t.co/yZR1ryE62j
Thrilled to announce my first first-author paper of grad school has been accepted at @eLife! Shoutout to @turcotteea, my 2 awesome labs, and my two amazing advisors Michael Emerman and @psmitchej. Check it out here: https://t.co/vIhW9EANOM
In a new study led by @CaelanRadford in collaboration with @FKlein_lab, we use an improved deep mutational scanning system to map the neutralizing specificities of broad human polyclonal serum antibodies from individuals living with HIV.
https://t.co/GCeUHRyjlQ
Final version of our paper on deep mutational scanning system to measure antigenic and functional effects of mutations to full SARS-CoV-2 spike: https://t.co/DDaCUk1MCx
System uses non-replicative lentiviral pseudotypes to safely map effects of thousands of spike mutations.
In work led by @bdadonaite @khdcrawford@CaelanRadford, we develop pseudovirus system to deep mutational scan full #SARSCoV2 spike: https://t.co/O6MGHXVWdS
TLDR: can now safely measure how all mutations to viral entry proteins affect antibody neutralization.
🧵 below (1/n)
In new work, we formulate a biophysical model of viral escape from polyclonal antibodies, & develop software to apply model to deep mutational scanning data to understand antigenic effects of combinations of mutations: https://t.co/6DS5PLqpJp
I explain in 🧵 below.
In new study, we examine how target cell ACE2 expression affects #SARSCoV2 neutralization https://t.co/47RTQ5aeSS
We find high-ACE2 cells emphasize role of RBD antibodies in serum & lower ACE2 cells reveal more neutralization by antibodies to other regions of spike.
🧵 below:
In new study led by @dbacsik, we show single influenza-infected cells produce wildly different numbers of viral progeny: https://t.co/mZSRW3k2iw
Surprisingly, cells that transcribe the most viral mRNA are NOT ones that produce the most progeny virions.
Explanatory 🧵 below
🥳 Excited to share that the Starr Lab is coming to the University of Utah @UofUBiochem! Check out the lab website to see where we’re headed in our studies of protein evolution in viruses and immunity https://t.co/J6Dc9pCBK3
We have new data on the effects of all RBD mutations to Omicron BA.1 and BA.2 on ACE2 affinity & RBD expression. These data, which are from experiments and analyses by @tylernstarr@AllieGreaney & Will Hannon are available here for viewing and download: https://t.co/QPXRmQIyZV