Jen Li

[Jen Li @JenLi67269204]

@JJForsterIII @NU_ChemE *clarification - our process is actually based on AA8, not AAV9

[Jen Li @JenLi67269204]

Group #6: Having trouble with our poster showing up publicly, so trying this again... #CHME4703Poster @NU_ChemE

[Jen Li @JenLi67269204]

Team 6: CDMO Manufacture of AAV with Continuous Harvest Technology and a Manufacturing Floor Plan #CHME4703Poster @NU_ChemE @michaelnguy14 @Robert_Newman97 @AntonRepnikov

[Jen Li @JenLi67269204]

@JJForsterIII @NU_ChemE Yes, our process is based off the process for producing AAV9. It definitely does come with some difficulties, which is why there are some yield losses in downstream. However, scalable cGMP manufacturing currently exists for AAV9 along with AAV2 and AAV8 (according to literature)

[Jen Li @JenLi67269204]

@ThomasJWebster1 @NU_ChemE It is difficult to ensure 100% of cells are lysed, but it is not a big deal since all the cell debris will be depth filtered out. Hope this answers your question! (2/2).

[Jen Li @JenLi67269204]

@ThomasJWebster1 @NU_ChemE Based off a typical AAV production process, lysis buffer is added so that it makes up about 10% of the reactor volume, followed by a mixing and hold step, which will ensure the majority of cells are lysed. (1/2)

[Jen Li @JenLi67269204]

@CocoonitL @NU_ChemE Great poster, very well laid out! The experimental results are very easy to understand and very clear. Now that you have some good results on the feasibility of this process, how can you ensure the scalability of manufacturing this food coating?

[Jen Li @JenLi67269204]

@TheRealNatural @cwribaudo @nour_naja @PateraMagdalini @CharlesChierico @NU_ChemE Awesome work! Really interesting results from your experiment. What is the operating temperature of your system, as being controlled by your temperature controller?

[Jen Li @JenLi67269204]

@sofiakcatalina @MaryLouNadeau @katelynripley @paigekru @AlexisDubs Great poster, very visually pleasing and well organized! Out of curiosity, how does the cost of transportation compare with the potential savings with your system?

[Jen Li @JenLi67269204]

Team 6: CDMO Manufacture of AAV with Continuous Harvest Technology and a Manufacturing Floor Plan #CHME4703Poster @NU_ChemE @michaelnguy14 @Robert_Newman97 @AntonRepnikov