Jing Ling

🎀Postdoc in @jinzhanglab | PhD in @ZhixingChen2 lab at PKU💃I Love biology🔬🧫🧬🐭

[DZ Gong | 龚 | ゴン | 공 @dz_gong]

Very fancy ratiometric voltage imaging with dual-polarity voltage sensors

Positive-going hybrid indicators for voltage imaging in excitable cells and tissues | Science Advances https://t.co/Bf8TfsY6uX

[PNASNews @PNASNews]

PK Mem dyes were designed as a gentle alternative to time-lapse fluorescence imaging of cellular plasma membranes. The dyes are less phototoxic and more photostable than existing plasma membrane dyes. In PNAS: https://t.co/cDx3pqNzqn

[Jing Ling @JingLing369]

@TsukijiLab @AxonsAlex Thanks!

[Jing Ling @JingLing369]

🚨 PUBLISHED in PNAS https://t.co/nmCNVry0pg

Introducing PK Mem: Gentle ✨, photostable ☀️membrane dyes for long-term live super-resolution imaging!

Great collaboration with Yitong Li and @AxonsAlex 🌟
Thank Peng Xi Lab & Hongyun Tang Lab for support!

[Zhixing Chen @ZhixingChen2]

Check it out at PNAS today. Thank you Alexandre for the collaboration!!

PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by @JingLing369, Yitong Liu, and all the collaborators!

Available @spirochrome

[Jing Ling @JingLing369]

Congrats🎉🎉🎉

[Deen Sun @DeenSun3]

Our biorxiv preprint is now online @nchembio 🎉
https://t.co/PIY7kk2APJ We made substantial revisions and added new experiments. Thanks to everyone who made this possible. Our journey continues!

[Zhixing Chen @ZhixingChen2]

Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!!

https://t.co/9QHbNAWZ5L

@spirochrome

[Nature Methods @naturemethods]

A suite of bridged rhodamine dyes for fluorescence microscopy, named BriDyes, offers excellent brightness, photostability, solubility, cell permeability, and resistance to photoblueing.

https://t.co/mW83CXqEYc

[Jin Zhang Lab @jinzhanglab]

Check out our new paper in @NatureBiotech sharing our latest kinase activity reporters! Led by former postdoc Michelle Frei, these reporters are based on HaloTag rather than an FP, for far-red imaging and superresolution! https://t.co/gTPnLXXQEV

[Erik A. Rodriguez @erin_rod_phd]

Today, I would like to honor the memory of Roger Y. Tsien, born on February 1, 1952. His legacy lives with all who use his technologies, including calcium sensors, fluorescent proteins, the acetoxymethyl (AM) ester, & many more!

https://t.co/YdWfBPUOBB

#FluorescenceFriday

[Yulong Li Lab @yulonglilab]

(1/7) Far-red dopamine sensor is coming! Excited to introduce our chemigenetic DA sensor, HaloDA1.0! #Dopamine #FarRedSensors, #GRAB https://t.co/hvFwIO6BWh

[Jin Zhang Lab @jinzhanglab]

Looking for a fluorescent biosensor and don’t know where to start? Want a comprehensive overview of the field? Check out our new review in @ACSChemRev online now as an ASAP article! From co-first authors @AMMGest @AyseZSahan @yanghao_zhong

https://t.co/YId0ttkqkf

[Spirochrome @spirochrome]

Our collaboration with @FluoSphera 2nd episode:

2 types of cancer spheroids (HER2 positive and HER2 negative) were color coded with our PKmem probes and co-cultured in microcavity plates.
Live cell imaging beautifully captures the progressive binding of trastuzumab-FITC (anti HER2 Antibody) only in HER2 positive spheroids.
This powerful platform, developed by FluoSphera, paves the way towards accurate drug discovery or personalized cancer treatment.

#Oncology #3DCellCulture #DrugDiscovery #Cancer

[Jing Ling @JingLing369]

😍😍😍

[Spirochrome @spirochrome]

🚨Spirochrome and @FluoSphera new collaboration!

Our PKmem probes for gentle plasma membrane imaging meet Fluosphera's highly innovative encapsulated cancer spheroids technology!

Staining lasted over 72 hours without (photo)toxicity or bleaching, improving overall data quality and facilitating tissue barcoding/segmentation! A perfect match between the two technologies.

😍Mini-supernovas💥under the objective!

More 🤯images and data soon 😉

[Zhixing Chen @ZhixingChen2]

I'm flattered everytime I see a multi-color movie with our gentle dyes together with mStayGold!

[Jing Ling @JingLing369]

🔬Gentle Rhodamines generally offer alleviated phototoxicity enabling long time-lapse imaging of different organelles
https://t.co/IztzThkAq1
Awesome collaboration with @TianyanL, @JulianKompa, Kai Johnsson group and @spirochrome
I've learned a lot from two great collaborators🥰

[Tianyan Liu @TianyanL]

Our paper introducing Gentle Rhodamine (GR) has finally been published.
GR probes make bio-samples happier 🥳 in long-term time-lapse microscopy🔬
Wonderful collaboration with @JulianKompa ,the Johnsson group,@JingLing970709, and @spirochrome
Thanks for all the authors!

[Spirochrome @spirochrome]

😍 PKmem 555 in action!

Thanks for sharing!

[Jing Ling @JingLing369]

@AxonsAlex @spirochrome We are very happy that PK Mem dyes can help you see the wonderful world of neurons!😝

[Jing Ling @JingLing369]

@ZihniLab Thanks a lot!😀

[Jing Ling @JingLing369]

🚨Thrilled to share our new pre-print about a gentle palette of plasma membrane dyes!
💥Alleviated Phototoxicity and Enhanced Photostability
🌟Versatile Compatibility
🔬Suitable for Long-Term Super-resolution imaging
#phototoxicity #SuperResolution #Microscopy #CellMigration

[Zhixing Chen @ZhixingChen2]

The MemBright PM stains set a high bar for specific membrane labeling. But if you care about the phototoxicity under time-lapse and SR imaging?

Here we present a gentler palette -- PK Mem dyes. Tailored for live cell SIM/STED!

https://t.co/BtwWu5uB25