JordanYorgason

Neuroscientist/CellBiologist/SoftwareEngineer. Neuroimmune, Limbic Neurcircuitry. Multiphoton microscopy/fscv/electrophys/ML. he/him

[JordanYorgason @JordanYorgason]

I feel like my lab just exploded with the start of this new semester and students returning. I've been so impressed with each of them and hope I can help their research experience be a meaningful one!

[JordanYorgason @JordanYorgason]

@ampolter I agree! The compartmentalization is one of the things I really liked about the IHC. Opens a lot of questions.

[JordanYorgason @JordanYorgason]

Thrilled to share latest pub examining effects of IL-10 on midbrain dopamine firing, terminal activity and learned behaviors. Click link for temporary free access. Article will also be made available on pubmed. Thanks to reviewers for excellent feedback! https://t.co/LpDQ8CS6zx

[JordanYorgason @JordanYorgason]

Direct faciliatory effects on dopamine terminals (as measured by voltammetry) were small, but significant. Microdialysis measures of dopamine in vivo were much larger and likely due largely to increased dopamine firing.

[JordanYorgason @JordanYorgason]

This excitatory effect is due to postsynaptic decreases in inhibitory currents.

[JordanYorgason @JordanYorgason]

First time labeling with a conjugated toxin (blue) worked out great. NAc GABA neurons in green

[JordanYorgason @JordanYorgason]

Excellent example of microglia chemoattraction from Erin Taylor and @_elizawhite in the lab.

[Tanner Fadero @TanFad]

How do you put multiple optics in a row and align them so that their optical axes agree? This is a thread where I show you how I build a compact Alignment Laser Assembly– the companion to my "Alignment Crane" from a few months ago:

https://t.co/uU8WBklP14

[JordanYorgason @JordanYorgason]

Our grape vines are growing spaghetti squash!!!

[JordanYorgason @JordanYorgason]

@TanFad @Dora_Mahecic That's a great idea. Also may need to consider some insulation for the electronics. Anodizing the aluminum helps, but may still want a thin insulation layer for the TI board if cradling in aluminum.

[JordanYorgason @JordanYorgason]

@Dora_Mahecic I don't have full access to the paper right now, but the authors may provide the stl files or may be willing to help mod to fit your board.

[JordanYorgason @JordanYorgason]

@Dora_Mahecic I'd try to mount like this. https://t.co/QHRINax7L8

[JordanYorgason @JordanYorgason]

The nipkow disk is showing up nicely tonight. Each hole is 40um in diameter and acts as a pinhole for the camera. Once the disk starts spinning, the optical sectioning starts happening.

[JordanYorgason @JordanYorgason]

submicron battle bots

[JordanYorgason @JordanYorgason]

@DavidBarker_PhD I hadn't looked at the Carter Shieh book chapter until today. Looks like a great resource. It also references a bunch of microscopy books including the James Pawley book.

[JordanYorgason @JordanYorgason]

@DavidBarker_PhD I prefer videos/online lectures. If book is preferred, James Pawleys Handbook on Confocal Microscopy is good. Covers wide and deep topical range. For general optics explanations I really like Edmund Optics catalog. Edmund website is great. Nikon/olymp edu websites are great too.

[JordanYorgason @JordanYorgason]

Excellent tutorial for using alignment cranes and stable posts.

[Tanner Fadero @TanFad]

1/? I've had a few people ask me why I prefer @Thorlabs posts over their cages. The argument for cages is self-defeating: supposedly they provide pre-aligned centration, but mechanical tolerances =/= optical tolerances. You end up having to put everything on kinematic mounts...

[JordanYorgason @JordanYorgason]

@_annaeverett First of many. That's a pretty sweet paper!