Nicolas Cardozo

Graduate Researcher | @UW @datamolecules @UwMolES @UWCSE

[GenomeTDCC @GenomeTDCC]

🚨Pre-Print Alert🚨
Single-molecule protein sequencing is the next frontier in sequencing technology.
Latest from @jeffnivala and colleagues - reading hundreds of amino acids and PTMs along protein strands AND re-read multiple times w/@nanopore arrays! 👇https://t.co/HrcqsWVyHC

[Nicolas Cardozo @NickCardozo1]

Fantastic work led by @KeisukeMotone, @DaphneKHeintz, and @jeffnivala! @datamolecules

Multi-pass, single-molecule nanopore reading of long protein strands with single-amino acid sensitivity https://t.co/0jZ0aBo6Mi

[Keisuke Motone / 元根 啓佑 @KeisukeMotone]

Our preprint is out! We hacked the @nanopore sequencer to read amino acids and PTMs along protein strands. This opens up the possibility for barcode sequencing at the protein level for highly multiplexed assays, PTM monitoring, and protein identification!
https://t.co/hVdNFa7ti4

[Jeff Nivala @jeffnivala]

Yesterday we released our preprint showing how a @nanopore array morphs into a protein sequencer... Achieving LONG reads (hundreds of amino acids) and the capability to re-read individual molecules multiple times, all thanks to a protein-processive molecular motor.

[Allen School @uwcse]

From data science, to machine learning, to molecular computing—and more—@UW #UWAllen faculty are shaping the future of our field. Discover how eight @NSF CAREER recipients are pushing boundaries and advancing #CSforGood. #NSFfunded #ThisIsUW #FacultyFriday https://t.co/7FALCtzh6v

[iScience @iScience_CP]

A review for our single molecule technology sequencing special issue from @KeisukeMotone at the @jeffnivala group - is sequencing proteins like herding cats? https://t.co/JKAp3TdFg3

[Nature Methods @naturemethods]

Research Highlight by @DrArunimaSingh: 'nanopore-addressable protein tags engineered as reporters' (NanoporeTERs) from the lab of @jeffnivala can be detected in multiplex on commercial sensor arrays.
Paper: https://t.co/ZLCQOyvU8m
Highlight: https://t.co/g4Xj5abkiR

[Keisuke Motone / 元根 啓佑 @KeisukeMotone]

Nanopore protein sequencing is a challenging frontier that has yet to be realized, like herding cats. Our review @iScience_CP covers the latest efforts and proposes further steps to this end. Many thanks to @jeffnivala @NickCardozo1 @datamolecules!

[Jeff Nivala @jeffnivala]

Pulling spaghetti decorated with Xmas ornaments through tiny holes is hard. Read our new review to do it at the nanoscale! Herding cats: label-based approaches in protein translocation through nanopore sensors for single-molecule protein sequence analysis https://t.co/Olaee49WAc

[Jeff Nivala @jeffnivala]

New Protein Reporters for MinIon Sequencer Point Toward Route for Single-Molecule Proteomics https://t.co/46W9utdiPg

[Nature Biotechnology @NatureBiotech]

Multiplexed direct detection of barcoded protein reporters on a nanopore array https://t.co/S7glG4fBB8

[Allen School @uwcse]

Co-authors @jeffnivala @nickcardozo1 @thekarenzhang @KatieDoroschak @luisceze @KStraussideas, Aerilynn Nguyen, Zoheb Siddiqui and Nicholas Bogard used @nanopore's portable MinION device and machine learning to interpret what the cells have to say: https://t.co/fsjLvZSRBQ 2/2

[Allen School @uwcse]

NanoporeTERs has entered the chat: In a new @NatureBiotech paper, @UW @MSFTResearch @datamolecules researchers unveil a new class of engineered reporter proteins for #biotech research that enable cells to “talk” to computers: https://t.co/6h6uMqCbms #UWinnovates 1/2

[Nicolas Cardozo @NickCardozo1]

Our paper on NanoporeTERs is finally out on @NatureBiotech! So proud of all the hard work @thekarenzhang @KatieDoroschak @nbogard @jeffnivala and others @datamolecules put in to make this happen! https://t.co/RV5eFg1shF

[Jeff Nivala @jeffnivala]

At last: NanoporeTERs pub is out @NatureBiotech! NTERs are a new class of multiplexable reporter protein that use @nanopore arrays to detect peptide-barcoded proteins directly at the protein level. Lots of juicy additions to this work since the preprint!
https://t.co/UIgGiIrPT9

[Nature Methods @naturemethods]

Single-cell methods are becoming the norm, but what about proteins? Chirlmin Joo and colleagues provide a detailed survey of the emerging landscape of single-molecule protein sequencing technologies in a Review out today! https://t.co/GpgUfuiRND

[Aishwarya Mandyam @Aishwarya_R_M]

Here's a list of fundraisers receiving donations for the covid crisis in India: https://t.co/axvg3mOW58

[Nicolas Cardozo @NickCardozo1]

#RSCPoster #MolEngPoster2020 Hi Everyone, this is my virtual poster that highlights non-invasive readout of lineage tracing experiments with NanoporeTERs!