Oliver Eichmüller

MD Student Studying Neurodevelopment and Disease @Knoblich_lab

[Catarina Martins Costa @catarinacsmc]

We're at the cover of Cell Stem Cell.
We're at the cover of Cell Stem Cell.
We're a-
(I can barely believe it. 🤯)

We found out why some people are born without the main interhemispheric bridge. 🧠

👏 to @PhDexheimer for this beautiful illustration of the corpus callosum.

[The Knoblich Lab @Knoblich_lab]

📢 Paper alert! Here's our latest @CellStemCell story, led by @catarinacsmc.

The corpus callosum safeguards interhemispheric communication in the human brain.
𝘈𝘙𝘐𝘋1𝘉 mutations cause agenesis/absence of the CC.
But by which mechanisms? ⤵
https://t.co/fmZbFCbLjo

🧵1/10

[Catarina Martins Costa @catarinacsmc]

🎙️✨ Sharing a glimpse of my research, in an interview to the Impact Award 2023.

I am so honoured to have received this distinction for my PhD work exploring the secrets of the human brain!
Thank you @univienna 🫶

Watch here ⤵
https://t.co/DtbD69dFKD
Subtitles in 🇵🇹🇬🇧🇩🇪

[The Knoblich Lab @Knoblich_lab]

📢 We’re live at the @embojournal!
🌐 https://t.co/P5Jy0WiLJn
Human telencephalic organoids cultured in the absence of Matrigel endogenously produce and self-organize the extracellular matrix, without affecting programs of neuronal differentiation.
Led by @catarinacsmc 👩‍🔬

🧵 1/5

[The Knoblich Lab @Knoblich_lab]

We are thrilled to share our latest work, a collaborative effort with @TreutleinLab, in Nature (https://t.co/pChgPCFEOM). We introduce CHOOSE, a powerful screening system within cerebral organoids, through which we identified developmental defects associated with autism.
🧵1/7

[IMBA imbavienna.bsky.social @IMBA_Vienna]

Does the human brain have an Achilles heel that ultimately leads to #Autism? With @TreutleinLab (@ETH_en) & @MedUni_Wien, @Knoblich_lab researchers led by @ChongLi_cl develop CHOOSE, a revolutionizing system to test multiple disease-related mutations in a single #brain #organoid.

[The Knoblich Lab @Knoblich_lab]

📣 Preprint alert!
Check out our latest story, led by @catarinacsmc.

The corpus callosum safeguards interhemispheric communication in the human brain.

𝘈𝘙𝘐𝘋1𝘉 mutations cause agenesis/absence of the CC.
We wanted to know why. 🤓

🧵1/9
https://t.co/RwkEOBpf31

[Catarina Martins Costa @catarinacsmc]

Thrilled to share this project with the world!
We looked into early morphogenesis of #brain #organoids and characterized the remarkable self-organizing capabilities of the neuroepithelium 🧠

Thanks to everyone in the @Knoblich_lab and beyond who supported me 💜
Go read it! 🤓😋

[The Knoblich Lab @Knoblich_lab]

📢Preprint alert! Here's our latest story, led by @catarinacsmc
Brain organoid morphogenesis can be driven by exogenous ECM (Matrigel), or self-sustained by endogenous ECM production. Late development is mostly independent of the initial ECM source
https://t.co/ps0u4MrJCt
🧵1/7

[Oliver Eichmüller @OEichmueller]

@krenn_veronica @Knoblich_lab Thanks @krenn_veronica 😌

[Nat Rev Neurology @NatRevNeurol]

New online! Human cerebral organoids — a new tool for clinical neurology research https://t.co/p2LlsKyiKW

[Oliver Eichmüller @OEichmueller]

Thanks @JRNLclub for featuring our work on tuberous sclerosis in #organoids and allowing me to share our science! 😌

[JRNLclub @JRNLclub]

Using human cerebral organoids, @OEichmueller and the @Knoblich_lab, uncovered a new interneuron progenitor cell that becomes dysregulated in tuberous sclerosis. @ScienceMagazine @IMBA_Vienna @oeaw @ViennaBiocenter @MedUni_Wien #JRNLclub https://t.co/Of9HCIKoCH

[The EMBO Journal @embojournal]

Issue 17 highlights:
Feeding activates gut #lipophagy
Biased #multipotency of Schwann cell precursors
ARF #GTPase interactors in lipid signaling
Bimodal Piezo #mechanosensor signaling
Cover by @PhilDexheimer features @Knoblich_lab #Gruffi resource
https://t.co/ZeNq2oMdJd

[The EMBO Journal @embojournal]

A unique stress signature found in in brain #organoid samples but not in fetal samples can be quantified and removed from #scRNAseq datasets using a new algorithm, Gruffi
New resource by the @Knoblich_lab @IMBA_Vienna
https://t.co/coCUXuO2Ia

[bioRxiv Immunology @biorxiv_immuno]

Carbon ion irradiation plus CTLA4 blockade elicits therapeutic immune responses in a murine tumor model https://t.co/SniyUjDa54 #biorxiv_immuno

[The Knoblich Lab @Knoblich_lab]

5/6 Gruffi can remove stressed cells, improving organoid datasets, which then reveal fetal developmental trajectories.

[The Knoblich Lab @Knoblich_lab]

4/6 Stressed cells are limited to a defined group, that we call stress-identity. It is a cellular state characterized by widespread metabolic changes beyond the activation of stress pathways, which is not found in vivo, therefore need to be removed from #scRNA data.

[The Knoblich Lab @Knoblich_lab]

3/6 We combined transcriptome-wide and gene-set based approaches in Granular Functional Filtering (Gruffi) and found that all analyzed brain organoids contain some stressed cells, but
their presence does not affect cell-type specification or neural maturation.

[The Knoblich Lab @Knoblich_lab]

2/6 Not all cells are created equal – In 3D tissue models, such as brain organoids oxygen & nutritient supply is limited in deep tissue. This leads to cell-stress, but it's extent and consequences are unclear.

[The Knoblich Lab @Knoblich_lab]

1/6 Stress in #brain #organoids is widely debated: Are all organoids & all cells affected? Does it exist in vivo? Does it impair cell differentiation? Gruffi, our new algorithm identifies and removes stressed cells from #scRNAseq #singlecell datasets. https://t.co/ewLtAZVsT4