Stefan Pfeffer

Cryo-electron microscopy, structural biology of molecular machines, ribosome-associated processes

[Stefan Pfeffer @PfefferCryoLab]

Congratulations to the shared first authors @martin_wuertz and Giulia Tonon! Fantastic collaboration with the Schiebel Lab @ZmbhH, @FabioLolicato at the BZH Heidelberg, @EustermannLab @embl, and the Erhardt Lab at KIT Karlsruhe. Thanks to all who contributed!

[Stefan Pfeffer @PfefferCryoLab]

We are excited to share our latest work elucidating the structural basis for how augmin binds to microtubules. Out just now @NatureComms:

https://t.co/zfSenm6j8C

[Stefan Pfeffer @PfefferCryoLab]

Nearly two decades after augmin's discovery in Drosophila, we have reconstituted the microtubule-binding region of the Drosophila augmin complex on microtubules and demonstrate the conserved mechanism by which the augmin subunit HAUS6 orients augmin for microtubule branching.

[Stefan Pfeffer @PfefferCryoLab]

Great collaboration with the Melnikov Lab @SergeyVMelniko1 @UniofNewcastle and the Hill Lab @chillzaa @YBRI_UoY. Congratulations to all authors, in particular the shared first authors Karla Helena-Bueno and Sophie Kopetschke @S_Kopetschke.

[Stefan Pfeffer @PfefferCryoLab]

Out just now at @NatureComms: using cryo-EM and cryo-electron tomography, we provide evidence that structurally heterogeneous ribosomes can cooperate in protein synthesis in bacterial cells:

https://t.co/Gg2qBHbL7W

[Sergey Melnikov @SergeyVMelniko1]

1/2 Here we unexpectedly find that protein S20/L26—identified at the dawn of ribosome research but then deemed a ribosome isolation artifact—is indeed a genuine component of the large ribosomal subunit. More on this ribosome content variation: https://t.co/lQZRcpdDmS

[Stefan Pfeffer @PfefferCryoLab]

@RalphSGrand @NatureComms Thanks a lot, Ralph!

[Stefan Pfeffer @PfefferCryoLab]

Online today at @NatureComms: in a major team effort, we reveal the structural mechanisms for centrosomal recruitment and organization of the human microtubule nucleator γ-TuRC: https://t.co/S7tLH4P16h

[Stefan Pfeffer @PfefferCryoLab]

A great collaboration with the Schiebel Lab @Zmbhh
@UniHeidelberg and the Gruss Lab @UniBonn. Congratulations to all authors, in particular the shared first authors Qi Gao, Florian Hofer, Bram Vermeulen @BramV98 and Sebastian Filbeck!

[Stefan Pfeffer @PfefferCryoLab]

Great collaboration with the Schiebel and Mayer Labs @Zmbhh @UniHeidelberg. Congratulations to all authors, in particular the shared first authors Anjun Zheng, Bram Vermeulen @BramV98
and Martin Würtz @martin_wuertz.

[Stefan Pfeffer @PfefferCryoLab]

Out just now at @NatureComms and a great start into the New Year: we reveal how yeast cytoplasmic γ-TuRC is assembled and activated by dimeric CM1 motifs of the receptor protein Spc72 and how the microtubule polymerase Stu2 binds to promote MT assembly. https://t.co/pvZvhGd7n1

[ZMBH - Center for Molecular Biology Heidelberg @ZmbhH]

Congratulations to Martin Würtz, who yesterday received the Nikon Young Scientist Award 2024 for excellent research in cell biology awarded by the German Society for Cell Biology.

[Stefan Pfeffer @PfefferCryoLab]

We have an open PhD position to explore structures of gamma-tubulin ring complexes using cryo-EM approaches here in our group @ZmbhH
@UniHeidelberg.

Please apply via the Heidelberg Biosciences International Graduate School @HBIGS_HD : https://t.co/0DB8fqfpJR

[Stefan Pfeffer @PfefferCryoLab]

We still have open PhD positions here in our cryo-EM group @ZmbhH @UniHeidelberg.

Please apply via the Heidelberg Biosciences International Graduate School or directly send us your application documents.

https://t.co/cJZCFXm0hI

[Stefan Pfeffer @PfefferCryoLab]

We have two open PhD positions to explore structures of ribosomes and gamma-tubulin complexes using cryo-EM approaches here in our group @ZmbhH @UniHeidelberg.
Please apply via the Heidelberg Biosciences International Graduate School @HBIGS_HD: https://t.co/cJZCFXlssa

[Stefan Pfeffer @PfefferCryoLab]

We have two open PhD positions to explore structures of ribosomes and gamma-tubulin complexes using cryo-EM approaches here in our group @ZmbhH @UniHeidelberg.
Please apply via the Heidelberg Biosciences International Graduate School @HBIGS_HD: https://t.co/cJZCFXlssa

[Stefan Pfeffer @PfefferCryoLab]

@RalphSGrand @embojournal Thanks, Ralph!

[Stefan Pfeffer @PfefferCryoLab]

Out just now @embojournal: our cryo-EM structure of the native RanGTP-induced γ-TuRC-capped microtubule, revealing a surprisingly asymmetric minus end architecture with direct implications for microtubule regulation and modulation during spindle assembly.

https://t.co/EqUpgynh9G

[Stefan Pfeffer @PfefferCryoLab]

@ChlandaL @embojournal Thank you, Petr!

[Stefan Pfeffer @PfefferCryoLab]

Great collaboration with the Schiebel Lab @Zmbhh @UniHeidelberg and the Gruss Lab @UniBonn. Congratulations to all authors, in particular the shared first authors Bram Vermeulen @BramV98, Anna Böhler and Qi Gao @QIGAO31314616.

[Stefan Pfeffer @PfefferCryoLab]

We still have ERC-funded PhD positions open in the lab! If you are interested, please apply!

[Stefan Pfeffer @PfefferCryoLab]

We have three open PhD positions funded under our #ERCStG 'RiboStress' to explore how cells remodel the activity and molecular organization of the translation machinery in response to stress.

If you are interested apply via the HBIGS graduate program: https://t.co/iwrwl1pkmL