Kaspar Podgorski

computational microscopy and tools for brain imaging.
Recently moved to @AllenInstitute from @HHMIJanelia

[Kaspar Podgorski @PodgorskiLab]

CT-inspired two-photon lets us record glutamate activity from hundreds of synapses at a time, at >1000 Hz. Really happy to share this, and there's more to come!
https://t.co/VAIi8GFTG0

[Kaspar Podgorski @PodgorskiLab]

@Nick_from_Texas @AllenInstitute @abhi_aggarwal1 These are spontaneous release events in TTX (called 'minis'). They occur randomly. Without TTX, we see larger, synchronous, often wave-like patterns. Example data available for download with the 2023 iGluSnFR3 paper. Our newest indicators, iGluSnFR4, are now out on bioRxiv!

[Andrew York @AndrewGYork]

Meet MagLOV: an engineered protein that responds STRONGLY to magnetic fields.

This is a fluorescence timelapse of MagLOV in E. coli. We're waving a (small) magnet under the plate.

Can you tell where the magnet is?

Want some? It's on Addgene now! https://t.co/eWs0aAIT1A

[Kaspar Podgorski @PodgorskiLab]

@WikiZong @voigtvision @timdweber @Thorlabs @brunopichler @Labrigger @ptrrupprecht @TeleVueOptics #67-251 Edmund

[Kaspar Podgorski @PodgorskiLab]

@voigtvision @timdweber @Thorlabs @brunopichler @Labrigger @ptrrupprecht @TeleVueOptics I don't- that's a great point and definitely worth knowing for the sensor size tradeoff!

[Kaspar Podgorski @PodgorskiLab]

@voigtvision @timdweber @Thorlabs @brunopichler @Labrigger @ptrrupprecht @TeleVueOptics In our SLAP2 microscope, we get efficient collection with a single-element condenser as the second element of the detection path telescope. The 3mm active area is just big enough for the shortest FL singlet condensers in the Edmund catalog.

[Kaspar Podgorski @PodgorskiLab]

@hod_dana @voigtvision @Thorlabs @brunopichler @Labrigger @ptrrupprecht *digitizer limits

[Kaspar Podgorski @PodgorskiLab]

@hod_dana @voigtvision @Thorlabs @brunopichler @Labrigger @ptrrupprecht Overall, excited. 3mm has lower dark counts than 6mm would, and it's not too hard to keep all your light in the 3mm. 6mm will be a better drop-in. Main worry is that they will underperform the SiPMs impressive dynamic range at high gain because of amplifier rails/detector limits

[David Wong-Campos @jdwong_campos]

I am thrilled to share my first bio paper ever in @AdamEzraCohen lab with super important contributions from @rhodamine110 lab!

We asked the question: How do neurons compute? We focused on L2/3 neurons.
Come for the science, stay for the videos! (1/17)

https://t.co/6zxtsAQzpZ

[Kaspar Podgorski @PodgorskiLab]

@KentaM_Hagihara Not to bias the poll, but what are the advantages of AAV9 over PhP.eB?

[Kenta M. Hagihara @KentaM_Hagihara]

We @AllenInstitute for NeurDyn-Ophys are gearing up to launch a platform for in vivo testing of genetic NM/NT/Voltage indicators. We want your opinion on the most suitable AAV serotype to deliver sensors to be tested. Which serotype do you think would be the best fit?

[Kaspar Podgorski @PodgorskiLab]

@Notas_medicina @AllenInstitute @abhi_aggarwal1 @naturemethods This point can be a bit contentious- protons are more abundant... but the most abundant NT molecule

[Philipp Leippe @PLeippe]

🎉 Thrilled to share 4 yrs of hard work: iAspSnFR preprint at https://t.co/h1PdrjkUz5 🔬 GFP-based aspartate sensor, engineered via deep mutational scanning, tested in HEK293 cells. Highly specific for intracellular aspartate! #iAspSnFR #biosensor #aspartate

[Kaspar Podgorski @PodgorskiLab]

@JimJohnsonSci @abhi_aggarwal1 @HHMIJanelia @AllenInstitute Yes! @abhi_aggarwal1 made a super-low affinity version and we are working with collaborators to apply it

[Kaspar Podgorski @PodgorskiLab]

@HasanU_JP @abhi_aggarwal1 @HHMIJanelia @AllenInstitute I saw an abstract (I believe from Torok group) about development of an ultrafast iGluSnFR3. Axonal localization was achieved by Kim et al (https://t.co/TBUNzpiWtv).
One of our iGluSnFR4 variants is *extremely* fast as well, but still to be tested in vivo. Stay tuned!

[Kaspar Podgorski @PodgorskiLab]

@AndrewHires @abhi_aggarwal1 @HHMIJanelia @AllenInstitute It's a tricky measurement to make. We haven't gone deep on it. Looking at amplitudes, some sites are very quantal, while others are not. There's been some nice work from @OertnerLab with older iGluSnFRs, would be exciting to see with iGluSnFR3.

[Abhi Aggarwal, MD @abhi_aggarwal1]

iGluSnFR3 is published! Check it out⬇️
https://t.co/hQC58oNxj2

We made a new glutamate indicator with improved sensitivity, kinetics & localization for imaging synaptic transmission.

This is #iGluSnFR3 in action detecting minis w/ TTX.
@PodgorskiLab @HHMIJanelia @AllenInstitute

[Kaspar Podgorski @PodgorskiLab]

Our group is looking for a Research Associate interested in helping test new fluorescent indicators in vivo. Fun team. Relocation support to Seattle. Know anyone? https://t.co/uGlnwERPm4

[Jaime de Juan-Sanz @JaimedeJuan]

Folks, first paper of our lab is out!!

We discovered that a couple of trans-synaptic proteins, LGI1 and ADAM23, are acutely translocated to the synaptic surface during firing!

This controls their abundance at clefts, impacting locally glutamate release.
https://t.co/NHDywvGrYR

[Addgene @Addgene]

We wrote a blog post in 2018 about the latest glutamate sensor - but times have progressed and so must we. @abhi_aggarwal1 updated our post to feature the new newest sensor: iGluSnF from @PodgorskiLab.
https://t.co/VI2NfuClsG

[Filip Tomaska @fltomaska]

https://t.co/zzcUIuTFvz