Raghava

Fundamental Biologist and Electrophysiologist.
Currently a Post-doc at University of Wisconsin-Madison

[Raghava @Raghava_Bio]

@tinakim_neuro @NaturePortfolio Congratulations Tina 👏

[Raghava @Raghava_Bio]

@prat_i_k Hi Pratik

Raghava here from Ed's lab. Congratulations on your new paper

[Raghava @Raghava_Bio]

Excited to be part of this new work from our lab led by the talented @nikunj_iitm and others.

[bioRxiv Neuroscience @biorxiv_neursci]

Alternative splicing of synaptotagmin 7 regulates oligomerization and short-term synaptic plasticity https://t.co/GHyMZpErFR #biorxiv_neursci

[Raghava @Raghava_Bio]

Congratulations @VidyadharDJ

[Cell Biology J-Club @cell_club]

Synaptic vesicle endocytosis deficits underlie cognitive dysfunction in mouse models of GBA-linked Parkinson’s disease and dementia with Lewy bodies
https://t.co/rfV3hICEVj

[Raghava @Raghava_Bio]

@tinakim_neuro Congratulations on HHMI Freeman Hrabowski scholar funding 👏

[Raghava @Raghava_Bio]

@tinakim_neuro @jessie_muir @DEOlsonLab @LinTianPhD Congratulations Tina and all the authors👏

[Raghava @Raghava_Bio]

@PraachiTiwari Hi Praachi, can you DM me?

[Raghava @Raghava_Bio]

@ViditaVaidya @NeuroCellPress @TIFRScience Congratulations on a great paper

[Raghava @Raghava_Bio]

@tinakim_neuro @naturemethods Congratulations Tina 👏

[Raghava @Raghava_Bio]

@mjhigley @jess_cardin Congratulations to you and @jess_cardin

[Raghava @Raghava_Bio]

I am happy to have contributed to this wonderful work by @actuallyeden during my previous post-doc in @johnalangray 's lab

[Raghava @Raghava_Bio]

D-Serine inhibits non-ionotropic NMDA receptor signaling
https://t.co/U0aDMN1rL0

[Raghava @Raghava_Bio]

D-Serine inhibits non-ionotropic NMDA receptor signaling
https://t.co/U0aDMN1rL0

[Raghava @Raghava_Bio]

@NeuroBill Thanks for your recommendations Bill, I would definitely consider DigitimerDS3 next, its looks great

[Raghava @Raghava_Bio]

@NeuroBill Hi
Can you help in understanding what causes this weird stimulus artefact? I get this sometimes, changing polarity works but the amplitude decreases
I am using ISO-Flex unit

[Raghava @Raghava_Bio]

@NeuroBill Hi Bill, did you miss this tweet from me earlier?
Thanks

[Raghava @Raghava_Bio]

@NeuroBill Hi Bill
I accidentally found the origin of the issue. I suspect the stimulus isolation unit (SIU) is the culprit here. These two traces are from the same cell 10s apart but with different SIUs. The graph on the right is a scaled version of the left one for better resolution

[Raghava @Raghava_Bio]

@Avishek92 @NolwennCloarec @letelliermathi1 @ebazbadillo Cool, what microscopy? Two-photon? I am planning for some slice imaging but we don't have a 2p in the lab. So thinking of other options.

[Raghava @Raghava_Bio]

Two Indian-origin professors, Venkatesan Sundaresan and Savithramma Dinesh Kumar from @ucdavis elected to the NAS, both from plant biology

[National Academy of Sciences @theNASciences]

We are thrilled to announce the election of 120 members and 24 international members to the National Academy of Sciences in recognition of their distinguished and continued achievements in original research.

Congratulations to our new #NASmembers and welcome to the Academy!

[Raghava @Raghava_Bio]

It's called an exit seminar at UC Davis, no defense!

[Raghava @Raghava_Bio]

@NeuroBill And those traces are AMPAR-only currents with NMDAR and GABA blocked by AP-V (50uM) and Picrotoxin (100uM), respectively.

[Raghava @Raghava_Bio]

@NeuroBill I have been using Iso-Flex for close to a decade but have yet to have this problem. This is due to the capacitive transients generated by the SIU. The unit I am using is a brand-new one with all-new batteries!
Any suggestions?