Rohatgi Lab

Official account for the Rohatgi Lab @StanfordMed.
Studying signaling pathways in development, disease and homeostasis.

[Rohatgi Lab @RohatgiLab]

Post-doc and research assistant positions are available in the Rohatgi lab in the areas signal transduction, lipid signaling/metabolism, primary cilia and biomolecular condensates. See detailed descriptions at https://t.co/ADDIA1iGlB jobRxiv 22119 Please RT

[Rohatgi Lab @RohatgiLab]

Check out this new pre-print from @pawelbiologist's lab!

[Pawel Niewiadomski @pawelbiologist]

Proud to present our latest preprint "The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium" by @Sylwia13042567 et al. in collaboration with @RohatgiLab https://t.co/XfmEwlhxnt #cilia #hedgehog

[Jennifer Kong, PhD @JennKong07]

Huge congratulations to ⁦@schmidt_lab — a 2021 Forbeck Fellow⁩!!!! 🎉🎉🎉🎉🎉🎉 https://t.co/gwuqWtH7tQ

[Broder Schmidt @BroderSchmidt]

Congrats @JennKong07, glad to see this out: https://t.co/ytDHrlNosT. Cool idea to tune development signaling in utero to manage birth defects.

[Jennifer Kong, PhD @JennKong07]

Yay! Our pre-print is finally out on BioRxiv! I am thrilled to finally share chapter 3 of our MMM saga: the mystery of MOSMO!

Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength https://t.co/yq2M8I98jn

[Broder Schmidt @BroderSchmidt]

New preprint on the mechanism of action (MOA) by which oxaliplatin kills cells, from the @RohatgiLab and @Onn_Brandman lab, both @StanfordBiochem! https://t.co/QNRcVOsZ8s

[Nature Chemical Biology @nchembio]

@RohatgiLab, Arun Radhakrishnan, & Christian Siebold review how the Hedgehog (Hh) receptor PTCH1 uses its transporter-like function to inhibit the GPCR SMO, with cholesterol as a ligand for SMO to effect downstream signaling. https://t.co/AOrGwQxDqV

[Jennifer Kong, PhD @JennKong07]

Woohooo!!! 🥳🎉 Our paper just went online @Dev_Cell! The major fundamental question we sought to address was, what are the mechanisms that regulate a receiving cell’s sensitivity to extracellular morphogens? 🧵🧵🧵
https://t.co/u0XbqVcZ7D

[Rohatgi Lab @RohatgiLab]

Congratulations Maia!

[Stanford Biochemistry @StanfordBiochem]

Maia Kinnebrew has received the 2019-20 Stanford Biosciences Excellence in Service to Graduate Students award! This award recognizes students who devote a large amount of time trying to improve the quality of the Stanford graduate student experience. Congratulations, Maia!

[Rohatgi Lab @RohatgiLab]

#developmentalBiology #cilia #signaling #ubiquitin

[Rohatgi Lab @RohatgiLab]

We welcome feedback on our characterization of a novel membrane-tethered ubiquitination pathway critical for the proper patterning of multiple tissues during development. In collaboration with the labs of Cecilia Lo, Teresa Gunn, and L. Aravind.
https://t.co/9AtQ9SqyEO

[Stanford Biochemistry @StanfordBiochem]

Check out this article by Ramin Dubey of the Rohatgi Lab and collaborators just published in eLife! "R-spondins engage heparan sulfate proteoglycans to potentiate WNT signaling" https://t.co/crTOYROqyd

[Rohatgi Lab @RohatgiLab]

Congrats to Ramin Dubey on his work linking lipid droplets to hydrophobic drug activation: https://t.co/1XwlLIbHMI.
We are grateful to our our collaborators and to @OlzmannLab for their News and Views on "enzymatically regulated phase partitioning": https://t.co/LQxvp2J2Ag

[Rohatgi Lab @RohatgiLab]

Membrane cholesterol accessibility as the messenger that communicates the hedgehog signal between patched and smoothened. Collaborative work with Arun Radhakrishnan's lab: https://t.co/l2DZ3dycB7
#gpcr #cilia #lipids #signaling #developmentalbiology #crispr

[Rohatgi Lab @RohatgiLab]

Broder's new paper presents a rigorous approach to test the connection between phase separation and function. Distinguished by the use of compositional mutagenesis to tune phase properties linked to hard biochemical readouts.
https://t.co/VPl7I3tT3j

[Broder Schmidt @BroderSchmidt]

Shorter title, new in vitro data, updated single-cell splicing reporter (available @Addgene): our work on TDP43 phase separation and splicing function is now online!

https://t.co/ELNY1Me8F4

@RohatgiLab @StanfordBiochem

[Rohatgi Lab @RohatgiLab]

Nice collaboration with the Siebold lab reveals the deep evolutionary connection between Hedgehog signaling and cholesterol transport-- congratulations to Maia Kinnebrew. Cholesterol is both a substrate and (when coupled to a protein ligand) an inhibitor of Patched!

[Nature Chemical Biology @nchembio]

Our new paper from @RohatgiLab and Christian Siebold’s group at @NDMOxford used structural and MD analysis to show that cholesterol inhibits Patched by inserting into the extracellular domain. Readable link at https://t.co/AR0H6s8TEn. https://t.co/njGOpVEDh1

[Rohatgi Lab @RohatgiLab]

We welcome feedback on @biorxivpreprint in collaboration with Radhakrishnan lab: sphingomyelin and accessible cholesterol regulate Hedgehog signaling at primary cilia #gpcr #cilia #lipids #signaling #developmentalbiology #crispr https://t.co/Ilnt9IyhZ5

[Rohatgi Lab @RohatgiLab]

A review from our own Jenn Kong on biochemical mechanisms of Hedgehog signaling, written with Christian Siebold, is out in Development-- includes an analysis of the many patched and smoothened structures. @Dev_journal https://t.co/1QGSanwFCb

[Stanford Biochemistry @StanfordBiochem]

The NIH has awarded Jennifer Kong of the @RohatgiLab the K99/R99 Pathway to Independence Award! This award aims to help outstanding postdoctoral researchers complete training and transition to independent, tenure-track or equivalent faculty positions. Congratulations, Jenn!