One major drawback of DNA force sensors is their super short lifetimeโmaking them rather unsuitable for most bio studies. Huge congrats (and thanks) to @IsaacLiLab for solving one more problem for the field! Looks like itโs time to image some integrin forces ๐
Latest from the lab:๐งฌDecoy DNA๐งฌcan extend the lifetime of DNA-based tension probes >24h by outcompeting DNases and preserving signal integrity in live-cell tension imaging - simple, general strategy for robust mechanobiology in DNase-rich environments. https://t.co/k8QmRYAIio
Hydrogels are essential in mechanobiologyโand PIC is a game changer. Fully synthetic yet highly biomimetic, PIC opens new doors for 3D cell culture. Huge congrats to Hongbo Yuan, Kouwer Lab & @Rocha_Lab on this detailed Nature Protocols guide! ๐๐ https://t.co/lTcSN1lC4h
What a view for science! โ๏ธโฐ๏ธ
#GRC on #Fibronectin#Integrins and Related Molecules is going strong at Renaissance Tuscany with a beautiful backdrop. I am happy to share my work on focal adhesion mechanics in my first-time Gordon conference with the brightest minds in the field.
New paper from the lab๐. Introducing Micro Immune Response On-chip (MIRO), a device that replicates tumors and their microenvironment to better understand responses to immunotherapies (cyan=immune cells, red=cancer, green=CAFs). @IBECBarcelona
New preprint ๐ฅ my PhD swan song, with @BauschLab @hamfaizi @PVlahovska@HaganLab (+ Matt, Sarvesh Layne & Aparna)
https://t.co/uVaq6sgNsq
We put a microtubules+kinesin gel inside a GUV and... this happens: crazy fluctuations of a "cell" induced by a "minimal cytoskeleton"!
We did it! ๐ช After years of fiddling with gels, lasers, cells, and infinite lines of custom codes, our first preprint is out! We integrated TFM & FRET-based tension probes into one workflow. ๐ฌ Already 400 downloads and Iโm only mildly panicking ๐ https://t.co/hAQicfoLHU
๐ง ๐๐ฎ๐ข๐ฅ๐๐ข๐ง๐ ๐ ๐ง๐๐ฐ ๐ฆ๐ข๐๐ซ๐จ๐ฌ๐๐จ๐ฉ๐ โ ๐๐๐ฒ #๐ ๐ฌ๐งช
@HofkensLab
โ First, we used the reference laser to project a perfect vertical line through the center of the objective holder and marked a target on the ceiling for future alignments
#Optics#Microscopy
These are exited times to be in. @FlimLabs creates portable and affordable FLIM devices and easy-to-use Phasor-FLIM analysis tools, turning your backyard microscopy to a cutting-edge technology. Really nice!
๐ฌ How does tumor stiffness affect cancer? Our latest research shows that stiffer colorectal tumors release proteins that fuel cancer cell migration and blood vessel formation, driving more aggressive tumor behavior. https://t.co/vNFUpKHscO
๐ฌ Proud to share our protocol for creating PAAm hydrogelsโyour new go-to method for studying mechanotransduction! ๐ฅ Customizable, efficient, and perfect for revealing how cells respond to mechanical forces. #Hydrogels#Mechanobiology
https://t.co/wvR5GoWWJN
๐Celebrating 5 incredible years with an unstoppable team! ๐ A huge THANK YOU to everyone whoโs been a part of this journey โyour hard work and passion have shaped our success. Iโm so proud of what weโve achieved together, and I canโt wait for whatโs next!ย Letโs keep going!๐ช
Congratulations to my dear colleague and partner in crime Sarah Vorsselmans for yet another win for FRET-based molecular tension probes. Well deserved @Sarah_baaah !๐๐๐
Thanks to the support of the Royal Belgian Society for Microscopy, we were able to hand out some awards on our #feeltheforce summer school.๐ Greta walked away with the award for the best talk, while @Sarah_baaah and Konstantinos shared the prize for the best poster.๐ Congrats!
Even when you feel dead inside, remember the Force is strong! "As for our ally is the Force, and a powerful ally it is." See you in next #feeltheforce summer school! (and I can't believe I am quoting SW, but there we go...)
As the #feeltheforce summer school '23 coming to an end, I would like to thank every participant, speaker and organizer to make this beautiful event possible. We are proud!
In today's #feeltheforce FRET-FLIM workshop, vinculin force sensors are imaged by Leica SP8 FALCON module, and the subsequent analysis is carried out by Phasor-FLIM approach using open access PAM software. Well done Sarah!