Murphy Lab

Structural and mechanistic study of proteins, especially redox- and metalloproteins

[Murphy Lab @_Murphy_Lab]

Happy to share that our work on the FDX2-bound core ISC complex is now published in @NatureComms 🎉

https://t.co/gfmRsx2TSd

By the way, we are now also on @bluesky! 🦋 https://t.co/D3AT4QtVjK

[Murphy Lab @_Murphy_Lab]

🎉 Our latest preprint is now online 👉 https://t.co/b8EYwcZZMx
Congrats to @RalfSteinhilper who determined #cryoEM structures of the ferredoxin 2-bound core iron-sulfur cluster assembly complex under reducing and turnover conditions at 2 Å resolution.

More below! 👇

🧵 1/8

[Murphy Lab @_Murphy_Lab]

Our elemental mapping work, combining cryo-EM and EELS to REEL analysis, is now also published on Nature Methods:

https://t.co/Yf3UGSGSWe

For a highlight “reel”, see here 👇

[Murphy Lab @_Murphy_Lab]

Happy to announce that our efforts to establish elemental mapping for cryo-EM ❄️ by EELS 🌈 are now shared on bioRxiv: https://t.co/hCTHQCyxN4
Congratulations to @zoliviapg and co-authors!

But wait - Can we map elements in 3D?
🧵👇 1/6

[Murphy Lab @_Murphy_Lab]

Congrats to @MaxYin_211013 and co-authors! Big thanks to X-ray team: Tristan Wagner, Olivier Lemaire and Mélissa Belhamri @MarineMicrobio; computational team: Gerhard Hummer and José Guadalupe Rosas Jiménez @HummerLab; MS team: Anna Shevchenko @mpicbg for the great collaboration!

[Murphy Lab @_Murphy_Lab]

How could CO₂ fixation occur in anaerobic and ancient life? Our new preprint sheds light on catalytic mechanism of the key enzyme CODH/ACS in the Wood-Ljungdahl pathway. Watch the summary movie to see how CO₂ is transformed into acetyl-CoA. Read more: https://t.co/pq42aOwLRF

[Murphy Lab @_Murphy_Lab]

The story doesn’t end there. QM/MM calculations reveal novel features of the catalytic metalloclusters, the C-cluster and A-cluster. The results suggest an electron storage mechanism for the C-cluster, and the configuration of the A-cluster in the ligand-free resting stage.

[Murphy Lab @_Murphy_Lab]

Happy to see our study on the structure of 46-kDa Streptococcus pneumoniae NOX out in @NatureSMB! Congratulations to the authors @PABLOSANSEGUND3 and @vicsnorr 🥳
Check it out here (open-access) 👉 https://t.co/HlpklHAeYr

[Murphy Lab @_Murphy_Lab]

Excited to share our preprint: “Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase” in which we obtained a structure of a 46-kDa membrane protein to 2.2 Å resolution without fiducials.
https://t.co/Cu2RxdxyZq

[Murphy Lab @_Murphy_Lab]

Many thanks to our colleagues @MPIbp and collaborators in the group of Roland Lill @Uni_MR for cooperation on this story and a study of the sulfur transfer intermediates in the frataxin-bound core ISC complex: https://t.co/EdUgKaF9hF

🧵 8/8

[Murphy Lab @_Murphy_Lab]

🎉 Our latest preprint is now online 👉 https://t.co/b8EYwcZZMx
Congrats to @RalfSteinhilper who determined #cryoEM structures of the ferredoxin 2-bound core iron-sulfur cluster assembly complex under reducing and turnover conditions at 2 Å resolution.

More below! 👇

🧵 1/8

[Murphy Lab @_Murphy_Lab]

Our study clarifies how FDX2 binds to the core ISC complex and confirms that de novo FeS biosynthesis is a highly concerted process. This might have implications for understanding the concentration-dependent effects of frataxin in diseases like Friedreich’s ataxia.

🧵 7/8

[Murphy Lab @_Murphy_Lab]

More research will be needed to reach single-atom sensitivity. In the meantime, we’d be grateful for any feedback and hope you enjoy the read!
Also, if you’d like to join the team: We do have an open post-doc position! ^^ https://t.co/lycMnKUnUr
🧵 6/6

[Murphy Lab @_Murphy_Lab]

Happy to announce that our efforts to establish elemental mapping for cryo-EM ❄️ by EELS 🌈 are now shared on bioRxiv: https://t.co/hCTHQCyxN4
Congratulations to @zoliviapg and co-authors!

But wait - Can we map elements in 3D?
🧵👇 1/6

[Murphy Lab @_Murphy_Lab]

A big thanks for their contributions, especially to @_Higor_Rosa, as well as Dietmar Riedel, @YuSebyChen, @FilipPetegem, and the always-helpful team from CEOS whose awesome CEFID we have been relying on, as well as @DECTRIS_News whose ELA has been crucial to this project.
🧵 5/6