Advanced Bioimaging Center @ UC Berkeley

A global collaborative center with groundbreaking imaging tools & technical expertise to tackle large scale imaging data projects

[UC Berkeley MCB @berkeleyMCB]

@Eric_Betzig @ABCUCBerkeley One microscope, every scale. MOSAIC images life from single molecules to whole organisms: dividing cells, remodeling organelles, regenerating zebrafish, Alzheimer's tissue at the nanoscale, live mouse neural dynamics. Credit: Fu, Liu, Milkie, Ruan et al., Nature Methods (2026).

[Eric Betzig @Eric_Betzig]

Congrats to all 49 authors on the publication of our paper on the multimodal MOSAIC microscope, to be found here: https://t.co/meZXjRMog2
A summary with movies I posted nearly a year ago can be found below.

[Nature Methods @naturemethods]

MOSAIC: a versatile multimodal microscopy that seamlessly transitions between light-sheet, 2-photon, label-free and super-resolution modalities. @Eric_Betzig @ABCUCBerkeley @legant_lab

https://t.co/l3NtYh3OrD

[Advanced Bioimaging Center @ UC Berkeley @ABCUCBerkeley]

We are recruiting an instrumentation scientist! https://t.co/MP9y66ygS0

[FocalPlane @focalplane_jcs]

🔬🔦Check out our ‘Imaging spotlight’ on Volumetric Imaging via Photochemical Sectioning (VIPS) from @Ruixuan3, Srigokul Upadhyayula and colleagues.

VIPS helps researchers scale super resolution imaging to large whole-mount tissues.
https://t.co/jhcTMYm99a

[Science Magazine @ScienceMagazine]

A new method called VIPS enables organ-wide nanoscale reconstruction for quantitative mapping of connectivity and molecular landscape in intact tissue.

Learn more this week in Science: https://t.co/ffOqBTWo5o

[Advanced Bioimaging Center @ UC Berkeley @ABCUCBerkeley]

This work was made possible in part by support from @cziscience @czbiohub @SloanFoundation @BerkeleyLab @HHMINEWS @ThePhilomathia @NIH @NERSC

[Advanced Bioimaging Center @ UC Berkeley @ABCUCBerkeley]

Thrilled to share our Science cover! VIPS (volumetric imaging via photochemical sectioning) eliminates working-distance limits for whole-mount, nanoscale imaging. LLSM + photodegradable hydrogel + petabyte-scale compute → axons & myelin across mouse olfactory bulbs (WT vs NPC1).

[Advanced Bioimaging Center @ UC Berkeley @ABCUCBerkeley]

Read more here:
https://t.co/b8S459ok1i #ExM #LLSM #imaging #Neuroscience

[Nature Methods @naturemethods]

Adaptive optical vision Fourier transformer (AOViFT): a machine learning framework for inferring image aberrations and restoring diffraction-limited performance.

https://t.co/hYlU9P2i8G

[Eric Betzig @Eric_Betzig]

1/ Better late than never, here’s a long thread of threads summarizing our MOSAIC preprint.
If you’re curious about the process by which a new microscope is conceived and brought into the world, this is the thread for you. If you’ve come for eye candy, there’s plenty of that too below.
https://t.co/BEfAolyeR9

[Advanced Bioimaging Center @ UC Berkeley @ABCUCBerkeley]

Finally here! Our preprint on the MOSAIC, a multimodal adaptive optical microscope enabling non‑invasive in‑vivo imaging from molecules to organisms, is out.
https://t.co/B2GHHP5b0K
@Eric_Betzig @legant_lab

[Reto Fiolka @RetoPaul]

The MOSAIC reconfigurable microscope by @Eric_Betzig et al is finally out!

MOSAIC integrates multiple advanced imaging techniques including light-sheet, label-free, super-resolution, and multi-photon, all equipped with adaptive optics:
https://t.co/RkwkoptVHv

[Chan Zuckerberg Biohub Network @czbiohub]

Unlocking the potential to image petabyte-scale datasets 😵‍💫! From #CZBiohubSF Investigator Gokul Upadhyayula and co-corresponding authors & @BerkeleyMCB colleagues Xiongtao Ruan and @Eric_Betzig.

[Advanced Bioimaging Center @ UC Berkeley @ABCUCBerkeley]

This work was led by Xiongtao Ruan and Matthew Mueller @ABCUCBerkeley @Eric_Betzig @berkeleyMCB @LBNLBioSci @LBNLresearch, and supported by @ThePhilomathia @czi @cziscience @HHMINEWS @HHMIJanelia @NERSC @SloanFoundation

[Advanced Bioimaging Center @ UC Berkeley @ABCUCBerkeley]

We are excited to introduce PetaKit5D, a comprehensive suite of software tools designed to tackle challenges in the handling of large datasets and make processing of petabyte-scale datasets feasible: https://t.co/P2WaWODV3F

[Advanced Bioimaging Center @ UC Berkeley @ABCUCBerkeley]

PetaKit5D is a scalable and efficient software for processing large datasets from light sheet microscopy and other imaging modalities. It is available for use in MATLAB and Python, as well as a GUI that requires no coding. Try it and share your feedback!
https://t.co/CkCbRndwPl

[Rita Strack @rita_strack]

Almost everyone in microscopy (especially LSFM) talks about data deluge, but the tools haven't kept apace with the problems. Check out PetaKit5D for processing and analyzing your huge datasets!

[Nature Methods @naturemethods]

Out today from Ruan, Betzig, Upadhyayula and colleagues! PetaKit5D offers versatile processing workflows for petabyte-scale light sheet microscopy data. https://t.co/vZUL2cMbHZ