A. Daniel Greene

Ecological entomologist with research interests in spatial ecology and statistics. I love being outside, playing and watching soccer, MTG, and board games 😃

[Tom Bilbo @tbilb]

it is here!! this short film turned out so well--deserving for such an illustrious career.

[Bento Lab 🧬 @theBentoLab]

Here’s a review of DNA extraction methods developed for point-of-need DNA-based testing that could be useful for DNA testing practitioners, students of PCR, fieldwork researchers, and anyone interested in DNA extraction chemistry.

The authors, Lee et al. (2023), review methods suitable for use outside of conventional laboratories, ranging from quick and dirty extractions to affordable and easy-to-use extraction testing devices for mass distribution, such as lab-on-a-chip devices.

They also dive into the chemistry and processes behind each extraction option with referenced examples, comprehensive tables, and some great illustrations of the different methods. So this could be useful even if all of your work is lab-based.

Importantly, the authors examine recent DNA extraction methods devices in the context of the World Health Organisation’s REASSURED criteria for point-of-need testing, and assess their usefulness for users within this framework.

You can read the article here:

Lee et al. (2023). Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review. Biosensors, 13(11), 980.
https://t.co/5gyoj3ndzn

Unfortunately, the authors didn't cover filter paper-based DNA extraction methods, which is a super-cheap and easy method which has been suggested as meeting numerous REASSURED criteria. You can find an example of this method below:

Aula et al. (2023). Optimisation of the DNA dipstick as a rapid extraction method for Schistosoma japonicum in infected mice samples and spiked human clinical samples. Infectious Diseases of Poverty, 12(1), 71.
https://t.co/4QOVUOgdsL

***

https://t.co/5gyoj3ndzn

[A. Daniel Greene @ADanielGreene1]

@buitengebieden @Savevidnow

[A. Daniel Greene @ADanielGreene1]

@TalkingTHFC The difference is that the quality of the rest of the team ensured that we would never win any of those competitions regardless of whether Kane turned up or not. When he goes to a real club and has a bad final, he will still win because the rest of the team aren't donkeys.

[Tom Bilbo @tbilb]

tune in tomorrow afternoon for a webinar on https://t.co/QcrZkCDi29 "How to manage spider mites in tomatoes with predatory mites" with myself and Jim Walgenbach (NC State)

https://t.co/7N2iC8L9Tk

[Tom Bilbo @tbilb]

Pretty neat video footage of the Clemson Extension REC stations and other campuses throughout SC. Great illustration of how Land Grant Universities like Clemson are much more than just their main campus.

#GoTigers

https://t.co/f2SZ2tIhX9

[Clemson Vegetable Weed Science @WeedClemson]

Dr. Ward and I are looking for a PhD student to lead a project focused on the ecological effects of implementing climate smart practices in leafy greens. Candidates with significant experience conducting field experiments are encouraged to apply ([email protected])

[A. Daniel Greene @ADanielGreene1]

@xaxiii90 @FutSheriff You just sign up for EA Play through Steam and then open the game and it works?

[Alexandra Revynthi @alex_revynthi]

Dr. Livia Ataide in her #postdoc focuses on the #lychee erinose mite and its interaction with the plant. Thank you Livia for your contributions to the lab, your passion and persistence with this challenging project! #PostocAppreciationWeek @UFTropical @UFEntNem

[Alexandra Revynthi @alex_revynthi]

Dr. Germán Vargas in his #postdoc focuses on #biological control of the #invasive #Hibiscusbudweevil. Thank Germán for your contributions to the lab, your passion and dedication! #PostdocAppreciationWeek @UFTropical @UFEntNem

[A. Daniel Greene @ADanielGreene1]

Hey that's me!

[Alexandra Revynthi @alex_revynthi]

Dr. @ADanielGreene1 in his #postdoc focuses on the behavioral and chemical ecology of the #invasive #Hibiscusbudweevil. Thank you @ADanielGreene1 for your contributions to the lab and your friendship! @UFTropical @UFEntNem #PostdocAppreciationWeek

[A. Daniel Greene @ADanielGreene1]

@alex_revynthi @UFTropical @UFEntNem It is an honor to work in the Revynthi Ornamental Entomology and Acarology Lab! Great friends and great research 👍 👍 👍

[A. Daniel Greene @ADanielGreene1]

It was a joy to be able to share the info we've learned on the biology, ecology, and management of the HBW! Thank you to all involved in the hibiscus industry for your support. Your feedback helps us immensely as we try to provide you with the most accurate info on this pest!

[UF IFAS Tropical Research and Education Center @UFTropical]

ICYMI Here is a preview of what took place at today's #HibiscusBudWeevil Workshop.

@ADanielGreene1's highlighted the importance of #Sanitation as a tactic for Integrated Pest Management (#IPM)

@alex_revynthi @UFEntNem #UFBugs

[A. Daniel Greene @ADanielGreene1]

Great opportunity here!

[Dr. David Coyle @drdavecoyle]

WHO WANTS A JOB?!? I'm looking for an ALB program technician. Will work closely with all ALB research projects (USDA, USFS, grad students) and will largely manage our ALB research operation. Reach out if you're interested and please spread the word! #academicjobs #technician

[A. Daniel Greene @ADanielGreene1]

Come on out!

[Alexandra Revynthi @alex_revynthi]

Have you registered for the upcoming in-person Hibiscus Bud Weevil workshop? If not, you still have time, follow the link https://t.co/leuRpPmiM0 CEUs will be available @evcampoverde @FNGLAMiamiDade @UFTropical @UFEntNem

[A. Daniel Greene @ADanielGreene1]

We had a great time sharing information on the entomological and acarological research that we conduct at the Tamiami International Orchid Festival! 😀

[A. Daniel Greene @ADanielGreene1]

800 hibiscus flower buds 🌺, because the Anthonomus testaceosquamosus colony is getting hungry!

[Classic Rock In Pics @crockpics]

Dave Grohl & Tenacious D. Photo by Jeff Kravitz.

[A. Daniel Greene @ADanielGreene1]

@alex_revynthi @EntsocAmerica @UFEntomology @UFTropical @FNGLAMiamiDade It was a lot of fun and well done to all! ❤️

[A. Daniel Greene @ADanielGreene1]

@alex_revynthi @data_question I can't even 😂