Anders Barth

Scientist at PicoQuant. Interested in all things single-molecule.

[Anders Barth @anders_barth]

@TheDNeva @cees_dekker Congrats!

[Evelyn Ploetz @EvelynPloetz]

🥳I'm excited to share our recent work on automated, single-molecule data analysis suitable for multi-labeled structures, called Deep-LASI! Congrats to all authors and thanks for this great collaboration between the Lamb group and @LabTinnefeld!

https://t.co/88WO1uF7Sl

[Anders Barth @anders_barth]

@prof_jelle @IvanMasl0v @TomKa_ehst @StijnDilissen @pedromlsilva @Sam_Duwe Agreed!

[Anders Barth @anders_barth]

Read the full story here: https://t.co/a3E6bOIPJB

This project received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant agreement no. 101029907.

6/6

[Anders Barth @anders_barth]

A side-project that evolved into a quite detailed exploration of zero-mode waveguide ‘nanowells’ for in vitro and in vivo (!) fluorescence microscopy.

Now out in @acsnano:
https://t.co/a3E6bOIPJB

Many thanks to @_SoraY and @MarvinTanenbaum for the nice collaboration!

1/6

[Anders Barth @anders_barth]

This was put into practice by @_SoraY who successfully grew cells on the palladium surface.
Even in the presence of micromolar cytoplasmic background signal (too high for TIRF), we could resolve the signal of single membrane-bound proteins diffusing within the ZMW nanowells.

5/6

[Cees Dekker @cees_dekker]

Latest #CDlab paper now online in @acsnano: https://t.co/GPKizeyFll

In collaboration with @MarvinTanenbaum's lab we developed zero-mode waveguides for detection of single molecules within live cells that protrude into the cavity.

1/

[Nicole Robb @nicole_robb1]

Come work with us! We are recruiting a 2-yr postdoc/research fellow for an exciting project using single-molecule microscopy to study viral replication. closing date 17th Sept. More info & how to apply here: https://t.co/geuqp1WK4Z

[Anders Barth @anders_barth]

@MiaoooYu Suppose it depends on the color of your lightsaber?😁
Congrats on the awesome work!

[Anders Barth @anders_barth]

@LipfertLab @KukuraLab @RunningPhoton I can at least confirm that (in our hands) calibration curves for protein and DNA ladders seem to coincide in terms of mass.

[Anders Barth @anders_barth]

@IvanMasl0v @CommsBio Congrats! An excellent showcase of the smFRET toolbox for a holistic understanding of such a complex system!

[Anders Barth @anders_barth]

Happy to see this one finally out, even though I merely had an “advisory” role in this story.

Congrats!

[Ivan Maslov @IvanMasl0v]

The main paper of my PhD, our smFRET study of sub-millisecond structural dynamics in A2A adenosine receptor (GPCR) is out in @CommsBio ! 🥳🥳🥳

https://t.co/BhHgtNnBHy

After two years from #preprint to publication I have no words

Sisyphus turned into Heracles, finally!

[Rita Strack @rita_strack]

Five years later we have a really strong follow-up study to this one (https://t.co/96w8GgJiuV) showing that smFRET data are reproducible enough to be trusted for dynamic structural biology applications of proteins.

[Nature Methods @naturemethods]

An multi-lab, blind study confirms that smFRET measurements on dynamic proteins are highly reproducible across instruments, analysis procedures and timescales, further highlighting the promise of smFRET for dynamic structural biology. https://t.co/EIPBxapRbG

[Anders Barth @anders_barth]

This was a ~5 year effort by the 47 (!) contributing authors.
Many thanks to all!

@LabTinnefeld @Craggs_Lab @DinaGrohmann @taekjip @prof_jelle @HugelLab @LemkeLab @eitan_lerner @EmmanuelMargeat @GeorgKrainer @AchillesKap @Michaelis_Lab @Schlierf_Lab

[Anders Barth @anders_barth]

The single-molecule #FRETcommunity seems to love international benchmark studies... so here is the newest one!
We tested the reproducibility of smFRET experiments on highly dynamic protein systems.
Spoiler: It still works remarkably well!
https://t.co/PtZmXgU4y1

@naturemethods

[Anders Barth @anders_barth]

Instead of a lengthy Tweetorial, first author Ganesh and I wrote a short summary of the paper, including our personal experiences along the way.

Read it here: https://t.co/KRJNMtsBr3

@ganesh__agam