Area-Gomez Lab @ CIB

Lab of Estela Area-Gomez at CIB-CSIC. Lipid metabolism in neurodegeneration with hints of MAMs, APP-C99 biology and having too much to say.

[Area-Gomez Lab @ CIB @areagomezlab]

Excited to be one of the Gold Prize recipients of the Oskar Fischer Prize @alzheimersprize

[UT San Antonio Research @UTSAResearch]

#UTSA Oskar Fischer Prize | Gold
~ Carlo Abbate, Fondazione @don_gnocchi, Florence, IT
~ Estela Area-Gomez, @Columbia, NYC, & @CIB_CSIC, Madrid, ESP
~ Bess Frost, @UTHealthSA, #SATX
~ Ralph Nixon, Nathan S. Kline Institute for Psychiatric Research, USA

https://t.co/vFaL69z0fB

[Area-Gomez Lab @ CIB @areagomezlab]

We will miss you too @_Rishi_Agrawal. Excited to follow your next adventure!

[Rishi Agrawal @_Rishi_Agrawal]

All robed up with the best PhD lab ever, @areagomezlab. Will deeply miss doing science with this team and amazing mentor. Thanks for an irreplaceable 6 years and teaching me how to do science -- and adulthood -- right!

[Area-Gomez Lab @ CIB @areagomezlab]

The first PhD of the Area-Gomez lab is complete! Congrats @_Rishi_Agrawal on a great presentation and defense!

[Rishi Agrawal @_Rishi_Agrawal]

A huge thanks to everyone who joined me for my defense talk last week, both in-person and virtually. I've been planning this talk for about 6 years.. And I'm happy it's over! All the messages and calls afterward were much appreciated 😊 #PhDone

[Area-Gomez Lab @ CIB @areagomezlab]

The latest preprint from our group is out! This is our first study in traumatic brain injury models. Please send any questions, concerns, criticisms or suggestions our way 😄🙏🏼🐁🏈

[bioRxiv Neuroscience @biorxiv_neursci]

Upregulated functionality of mitochondria-associated ER membranes in a mouse model of traumatic brain injury https://t.co/W29sz4yKq4 #biorxiv_neursci

[Jen Heemstra @jenheemstra]

As students and postdocs face a challenging and uncertain job market, it is more important than ever for faculty mentors to remember that all career paths are valid career paths and that our goal is to help mentees achieve their career goals, not replicate ours.

[Area-Gomez Lab @ CIB @areagomezlab]

Let's go women in STEM!!

[The Nobel Prize @NobelPrize]

BREAKING NEWS:
The 2020 #NobelPrize in Chemistry has been awarded to Emmanuelle Charpentier and Jennifer A. Doudna “for the development of a method for genome editing.”

[Area-Gomez Lab @ CIB @areagomezlab]

We had a great time presenting at the
@ColumbiaMed @TaubInstitute annual retreat today. PI Estela and postdoc Jorge Montesinos presented our recent work reported in @embojournal. Thanks for the invitation, and for organizing an outstanding agenda of speakers!

[Area-Gomez Lab @ CIB @areagomezlab]

@rikvanderkant @LeventalLab @brainychickJ We grow the cells to confluency. Minimum 6 plates (start with that), but 10-12 might be necessary. We usually focus more on MAM yields than bulk ER yields though so maybe you won't need as many plates as we do

[Area-Gomez Lab @ CIB @areagomezlab]

@rikvanderkant @LeventalLab @brainychickJ The only suggestion specific to neurons we'd have is that you will have to grow a lot of cells to obtain a workable quantity of ER. We're not sure what you're working with, but multiple 15cm plates would be advisable if possible.

[Area-Gomez Lab @ CIB @areagomezlab]

@rikvanderkant @LeventalLab @brainychickJ Hey! Agreed with @LeventalLab that "pure" is a strong word. We like to say "enriched" 😃 We've never tried the thermofisher protocol but here is our recent subcellular fractionation protocol, which you can follow to obtain "Bulk ER" fractions. https://t.co/bISEbWGXPn

[Tiago Gil Oliveira @TGO_lab]

Today is #WorldAlzheimersDay. It’s a day to specially think about the patients and family members that are affected. And a day to acknowledge the work from doctors and scientists to #ENDALZ

For more info on Alzheimer’s disease:
https://t.co/jRffkPwSCq

[Area-Gomez Lab @ CIB @areagomezlab]

For a more in-depth explanation of this hypothesis, we would like to refer you to a recently published book chapter. A huge shout-out to everyone behind this work! Thanks for reading, and we're looking forward to discussing!

https://t.co/hJQoKdBCZI

[Area-Gomez Lab @ CIB @areagomezlab]

We're excited to share our recent work describing roles of APP-C99 in regulating cellular cholesterol trafficking, with relevance to Alzheimer's disease. We hope this work helps connect multiple pathologies observed over decades of AD research.

[The EMBO Journal @embojournal]

ER delivery of a beta-#secretase-cleaved APP fragment C99 turns it into a lipid sensor facilitating formation of detergent-resistant #mitochondria-associated ER membranes (MAM) – @areagomezlab @ColumbiaMed
https://t.co/p6jvdh9Mq5

[Area-Gomez Lab @ CIB @areagomezlab]

We hypothesize that increased C99 levels and cellular cholesterol levels converge at the upregulation of MAM activities early in Alzheimer's pathogenesis to disrupt neuronal homeostasis.