Caroline Wilson

(she/her) @UCSF Tetrad phd candidate with @LukeGilbertSF | new yorker and genome engineer formerly @nyscf and @colbycollege

[LukeGilbert @LukeGilbertSF]

We mapped gene interactions across different environmental conditions (GxGxE) at scale for the first time in human cells. These maps lead to the realization that many genes function in a context dependent manner which provides insight into how humans have relatively few genes but many cell types. Congratulations Ben!
Paper:
https://t.co/w5bYZUUK4n

[Michael Kosicki @michaelkosicki]

Probe-based scRNA-seq + makes modern perturbation genomics cheap and flexible - highlighting ProPer-seq m/s I was lucky to contribute to @LukeGilbertSF's lab https://t.co/oezInB6QBN

[LukeGilbert @LukeGilbertSF]

excited about therapeutic biology? consider joining us!
https://t.co/8pG3Ggwqat

[Ansu Satpathy @Satpathology]

Happy to share our new work describing DESynR genes!

The evolution of new human protein-coding genes relies on repurposing protein domains from ancestral genes, rather than de novo sequence creation. New genes evolved from old parts, and while we have methods that enable high-throughput generation and screening of base-pair level sequence evolution (mutagenesis, directed evolution, CRISPR, etc), we lack similar scalable technologies for domain-level evolution.

We developed a high-throughput combinatorial molecular assembly method to generate thousands of barcoded novel genes from the domain building blocks of natural genes, termed DESynR (Domain Engineered via Synthesis and Recombination) genes.

[Caroline Wilson @carwils]

COMET! We generate libraries of combinatorial dCas9-based fusion proteins that modulate transcription at endogenous loci.

[LukeGilbert @LukeGilbertSF]

excited to share COMET! A scalable CRISPR approach we (@carwils) developed to identify multidomain proteins that modulate transcription at specific endogenous genomic loci. @arcinstitute @ucsf
https://t.co/Qhe38N1zzj

[Chris Hsiung 熊 @hsiung_chris]

Our preprint reporting a multiAsCas12a CRISPRi platform for >3plex combinatorial targeting of genes and/or enhancers (including 6plex in high-throughput pooled screens) is now a peer-reviewed publication! https://t.co/upQfZJfNCQ.

[LukeGilbert @LukeGilbertSF]

excited about multiAsCas12a which we (@hsiung_chris) show enables 6plex CRISPRi functional genomics experiments @arcinstitute @ucsf
https://t.co/GRid14dKi8

[Caroline Wilson @carwils]

@lysergicdesigns @naturemethods @lysergicdesigns have you had success delivering any of these via lenti?

[Caroline Wilson @carwils]

An incredibly sad loss of such an extraordinary woman and advocate. Susan transformed stem cell research and her legacy will continue with those touched by her vision for NYSCF.

[New York Stem Cell Foundation Research Institute @nyscf]

With a very heavy heart, we announce the passing of our CEO and co-founder, Susan Solomon, following a long battle with ovarian cancer. We owe all that NYSCF has accomplished to Susan, and no words can express our gratitude and awe for her catalytic impact on stem cell research.

[Caroline Wilson @carwils]

this crossword clue today made me 😠😠

[Caroline Wilson @carwils]

@joeBondyDenomy *looks away from Tetrad macro Ni-NTA problem set question* *opens twitter* *immediately sees macro problem set*

[Caroline Wilson @carwils]

sci twitter q: what is the best way to manage scientific papers? What are the pros and cons of your pick? I'm a Papers user, but trying to decide if I should make a switch before beginning my phd!

[Caroline Wilson @carwils]

thinking about my transition to grad school and the first thing I thought about this morning was whether or not I’ll have my own premium snapgene subscription

[Caroline Wilson @carwils]

Absolutely elated to share that I’ll be joining the Tetrad Program @UCSF this fall!!!

[Caroline Wilson @carwils]

tissue culture and taylor swift is about to be *chef’s kiss*

[bioRxiv @biorxivpreprint]

Multiplexed activation in mammalian cells using dFnCas12a-VPR https://t.co/xWp2lXs972 #bioRxiv

[Xiaokun Shu @ShuLab2010]

We have discovered a potent drug that blocks SARS-CoV-2 by a distinct mode of action: 1) EC50 ~ 80 nM by plaque assay; 2) it inactivates viral particles; 3) drug-treated coronavirus no longer binds to host cells. The drug is ethacridine. 1/n https://t.co/zrBi6qp5eK

[Caroline Wilson @carwils]

It’s #WorldCRISPRDay so naturally two browsers to catch talks from both @MartinUCSF and @omarabudayyeh & @jgooten are required 🧬✂️

[ChristofferFlensburg @CFlensburg]

@morgantaschuk We find more significant p-values when the wet lab postdoc does the analysis.

[Caroline Wilson @carwils]

@fish_follower Sci-hub!! @fish_follower