Charlie Roco

CTO and cofounder @ParseBio. Co-developer of single cell combinatorial barcoding method SPLiT-seq. Making genomics more scalable, flexible, and accessible.

[Charlie Roco @CharlieRoco]

amazing

[Rahul Satija @satijalab]

Check out our two new papers with new single-cell tech/methods/data!

1. Phospho-seq: Multi-modal profiling of intracellualar proteins
https://t.co/6z5rx0UeNU

2. Systematic Perturb-seq of signaling regulators (2.6M cells, 6 cell lines, 1500 perturbations)
https://t.co/PPc31sW0dO

[Hannah Hrncir @HannahHrncir]

So excited to work with @ParseBio to share my work studying the role of Sox9 in promoting branching morphogenesis in mouse intrahepatic bile ducts (IHBDs).

I will highlight how Parse scRNA-seq enabled discovery of a target to rescue IHBD branching in mice lacking Sox9.

[Parse Biosciences @ParseBio]

Are you attending #ASHG23? Don't miss our featured education session with Rahul Satija (@satijalab) from the New York Genome Center. Lunch will be provided for the first 50 attendees.

[Parse Biosciences @ParseBio]

📣 "Single cell sequencing using Evercode shows a superior outcome compared to 10x Genomics™"

Watch the full video here 👉 https://t.co/c2dKKALp2N

#comparisonstudy #research #singlecell

[Charlie Roco @CharlieRoco]

Looking forward to it!

[Front Line Genomics @FLGenomics]

Join @CharlieRoco, Co-Founder and CTO of @ParseBio as he reveals important insights in his talk, "Expanding Capabilities in Single Cell RNA Sequencing".

Don't miss this opportunity to gain new perspectives at #FoGBoston #RNA #SingleCell

Find out more: https://t.co/KQmfdXPXt7

[Daniel V Brown @dvbro0]

Happy to present our new preprint! We ran a head-to-head comparison of sample multiplexing reagents for single-cell RNA-Seq on the same samples on the same day. 1/9 https://t.co/c0gK98224B

[Irit Miller 🎗️ @MillerIrit]

That was nuclei extraction extraction, using @S2Genomics singulator and fixation w/ @ParseBio kits (lucky 13 kits!). Looking forward to the next steps 👩🏻‍🔬
And how was your week? #postdoclife #biology

[Charlie Roco @CharlieRoco]

Big thanks to @ToonSwings and the entire #RNGS23 organizing committee for inviting me to represent @ParseBio in beautiful Ghent! What a great conference - hope to be back again soon!

[Parse Biosciences @ParseBio]

Get ready. https://t.co/p39t2Wbh0E

[Charlie Roco @CharlieRoco]

@nomad421 @mikelove Looks like this paper from 2016 had the same conclusion

https://t.co/Ggox6VLpU2

"For future RNA-seq experiments, these results suggest that at least six biological replicates should be used, rising to at least 12 when it is important to identify SDE genes for all fold changes."

[Alex Rosenberg @dna_rosenberg]

1/ We previously generated a 1M cell scRNA-seq dataset with 24 Type1 Diabetes/Control PBMC samples. Now Daniel Diaz, one of our Bioinformatics Application Scientists, has built a tutorial showing how you can easily analyze this dataset using existing single cell tools.

[Parse Biosciences @ParseBio]

You can now do a deep dive into our 1 million cell T1D dataset! It was generated from 24 samples fixed over the span of 3 weeks and barcoded all together in a single run. The donor data was integrated with Harmony to generate the UMAPs. #singlecell https://t.co/deauJ5Gvtz

[Jeremy Simon @jeremy_m_simon]

FYI this vignette has now been updated with some of our more recent recommendations for handling SPLiT-seq/ParseBio data with {alevin-fry}. Namely: it's important to quantify in U+S+A mode with a splici reference, and properly import all those counts into R with {fishpond}

[Charlie Roco @CharlieRoco]

@jeremy_m_simon Thank your for expanding compatibility to Parse!

[Parse Biosciences @ParseBio]

We had a blast helping to teach a course on #SingleCell profiling and analysis in #neuroscience to the next generation of researchers with #Cajal! The future of single cell is bright! @cgfbordeaux @Neuro_Bordeaux @HjerlingLeffler @Cajal_Training @AMunozManchado @KharchenkoLab

[Charlie Roco @CharlieRoco]

@methylnick @ParseBio @Decode_Science It was great chatting with you and the rest of the team!
@LGMartelotto looks like you've got some competition 😀

[Bordeaux School Of Neuroscience 🧠 @bdxneuroschool]

🎙️@KharchenkoLab & @ParseBio will give their talks as part of our #Cajal course on Single #cell profiling and analysis in #neuroscience
📍@cgfbordeaux
🎟️Free entry

@Neuro_Bordeaux @HjerlingLeffler @Cajal_Training
@KharchenkoLab @AMunozManchado

[Charlie Roco @CharlieRoco]

@LGMartelotto @Decode_Science @ParseBio Was great to finally meet in person! Thanks for showing me around. Until next time!

[Tyler Alban @TylerAlban1]

First mega kit through @ParseBio and the sub library looks great after sequencing! So convenient to run sub libraries and know what’s going on before the big run. Estimating close to 900k cells for this exp!! Looking forward to the final results but so far so good.🤞🏼

[Charlie Roco @CharlieRoco]

@Al__Forrest Likewise, it was great to learn more about all of your work!