Gray Huffman

Graduate Researcher with the Slavov Lab

[Gray Huffman @GrayHuffman]

@edemmott Way to go, @edemmott!

[Gray Huffman @GrayHuffman]

@roumenina @slavov_n @NUBioE1 @slavovLab Hi Professor Roumenina, thanks for your interest in attending this talk! I'll send you the Zoom link momentarily.

[Gray Huffman @GrayHuffman]

Head over to https://t.co/5GZHgjcbtL to try out the newest version of @TheCoxLab and @quiztoph's excellent software platform for targeted and prioritized data acquisition!

It was a pleasure collaborating with you guys, as well, @quiztoph!

[Slavov Laboratory @slavovLab]

We always aimed to link single-cell protein measurements to cellular functions and regulatory mechanisms.

This project was a major step for us in this directions, especially with the direct measurements proteolysis products: https://t.co/uccyi8wgTT Data: https://t.co/BIIiCyDKIH

[Gray Huffman @GrayHuffman]

@slavov_n @EastCoast55 @EastCoast55, thanks to @quiztoph and @TheCoxLab, prioritized analyses are no more difficult to set up than your standard MS method.

Let me know if you'd like to set up a Zoom call to chat about the workflow

[Gray Huffman @GrayHuffman]

@Lo_Zanzi @Marco_Di_Gioia @NASPGHAN @CrohnsColitisFn Way to go, @Marco_Di_Gioia!

[Prof. Nikolai Slavov @slavov_n]

Did you think that data completeness of DIA could be improved?

[Jason Derks @_JasonDerks]

I'm very excited to share the work we've done @slavovLab in collaboration with @DemichevLab! Increasing the throughput of sensitive proteomics by plexDIA
https://t.co/CSTKeZ5L9P

[Prof. Nikolai Slavov @slavov_n]

Yes, stay tuned ... more is coming, ... and it cannot be soon enough.

[Prof. Nikolai Slavov @slavov_n]

.@_lukekhoury and I highlight the identification of a signaling interactome and outline how MS proteomics can be combined with deep learning towards comprehensive identification of regulatory protein networks https://t.co/ZVaWC6YaVd

[Gray Huffman @GrayHuffman]

@_AndrewLeduc's talk on nanoliter-scale single-cell sample prep just went up on YouTube.

A great presentation for anyone interested in learning about the benefits of small-volume workflows!

https://t.co/u4R89jasHE
#cellenONE #proteomics

[Gray Huffman @GrayHuffman]

Interested in evaluating your own single-cell proteomics data sets with a user friendly and well documented pipeline?

Check out the scp R package and accompanying preprint from @c_vanderaa and @lgatt0:
https://t.co/vkomYEtn3O

#SingleCell #Bioinformatics #proteomics

[Gray Huffman @GrayHuffman]

@KentsisResearch @slavovLab Thanks so much, @KentsisResearch; looking forward to catching your talk tomorrow afternoon!

[Ed Emmott @edemmott]

Led by @harrisonspecht and just in time for #scp2019, our next-generation version of single cell proteomics: SCoPE2 is now out on bioRxiv! In it we apply SCoPE2 to the study of macrophage differentiation. https://t.co/RXNQ58OnqV

[Sara Rouhanifard @SRouhanifard]

Thanks for coming to my twitter poster! Development of click-amplifying FISH (clampFISH) probes to exponentially amplify RNA fluorescent signal in situ with single molecule resolution. All questions and comments welcome! @arjunrajlab #RSCPoster #RSCChemBio

[Gray Huffman @GrayHuffman]

Here's a preview of the DO-MS dashboard in action:

[Gray Huffman @GrayHuffman]

If you're interested in optimizing your LC-MS/MS-driven #proteomics workflows, we hope you'll check out DO-MS, an opensource R-based dashboard for visualizing search results #RSCPoster #RSCChemBio #RSCAnalytical

[Gray Huffman @GrayHuffman]

...whereas cross-cell-type peptide abundances are not.

This type of plot can serve as a useful metric for determining the accuracy of quantitation, as well as for determining potential sample prep issues, such as poor label quenching (for which figure a. can also be useful).