Top Tweets for #KGSlabEvolves
Oh no, a mistake from my first day back in lab; too little media! What do you think @GrantKinsler @PetrovADmitri Is this as bad as the #PetrovEffect in #1BigBatch? Still 3 timepoints to go! Fortunately @KaraSchmidlin @SamApodaca_ and @dnewell_21 are in charge! #KGSlabEvolves
Today I pipetted! First time back in the lab after a long time and I really missed it. @KaraSchmidlin and @SamApodaca_ evolved yeast to resist different drugs are now conducting a big screen for cross resistance/sensitivity. More updates soon! #KGSlabEvolves
The remaining time points arrived at Macrogen for sequencing! Library prep was partially done with the biomek i7. What a life (thumb) saver! I cannot wait to have a complete data set from this experiment! #KGSlabEvolves @KSamerotte @SamApodaca_ @ASUBiodesign
Last transfer of the evolution completed! After we collect our last time point on Friday we'll be moving onto analyzing barcode frequencies next year. #KGSlabEvolves @KaraSchmidlin @KSamerotte @ASUBiodesign
Today was our last transfer. We will collect TP25 on Friday and this evolution will be complete! I’m looking forward to processing NGS data and identifying mutations! More to come next year. #KGSlabEvolves @KSamerotte
Thanks @SamApodaca_ for all your hard work and dedication.
Library prep day! Thanks @SamApodaca_ and @LeandraBrettner for all the help! We prepped 5 time points today and I’m sending them off tomorrow. Nothing like enjoying a break while your barcodes are sequenced. #KGSlabEvolves @KSamerotte @ASUBiodesign
We completed time point 22 today and did our last weekend transfer! We can't believe there's only about a week left in our evolution now! #KGSlabEvolves @KaraSchmidlin @KSamerotte @ASUBiodesign
Last weekend in the lab! Previous work by the @SashaFLevy lab observed >3000 highly beneficial mutations in low glucose conditions. Could we see that many beneficial mutations or do drug conditions reduce this number? We will find out soon! #KGSlabEvolves @KSamerotte @SamApodaca_
@SamApodaca_ and I finished time point 21. We have DNA extracted for time points 12, 14, 16, & 18. Library prep next week and off to sequence before the holidays! I can't wait to get data back! More on the library prep process next week. #KGSlabEvolves @KSamerotte @ASUBiodesign
Made it through time point 20 today! Only about a week and a half of transfers to go! #KGSlabEvolves @KaraSchmidlin @KSamerotte @ASUBiodesign
Time point 20 completed today! Sam and I can hardly believe it. Also, our cryovials FINALLY came in! We have matching color tops again! (Finding joy in the small things!) #KGSlabEvolves @SamApodaca_ @KSamerotte @ASUBiodesign
It’s Friday! Time point 18 was completed which means we are two weeks away from completion! 🎉 Also, I’m busy processing NGS data from T0! Hopefully I’ll be able to share a sneak peek soon. #KGSlabEvolves @KSamerotte @SamApodaca_ @ASUBiodesign
Time point 17 complete! We're over halfway through the evolution now and our cell counts have been pretty interesting, check out @KaraSchmidlin's tweet from today for more info and some graphs! #KGSlabEvolves @KSamerotte @ASUBiodesign
Transfer 17 complete! By tracking cell densities we have noticed counts are changing in different ways across different environments. D2, the no drug condition, continues to result in reduced counts. Not sure what this means. Any ideas? #KGSlabEvolves @SamApodaca_ @KSamerotte
Happy Monday! Here is your transfer of the day. T16 completed. #KGSlabEvolves @SamApodaca_ @KSamerotte @ASUBiodesign
We are officially half way done with the evolution experiment!
Time point = 15
Generations ~120
Here is the setup for making glycerol stocks. We centrifuge 50 ml at a time and resuspend the pellet in 20% glycerol. #KGSlabEvolves @KSamerotte @SamApodaca_
Happy Thanksgiving everyone! Time point 14 completed today. Thankful for the opportunity to work on this project in an amazing lab and that it's going well so far! #KGSlabEvolves @KSamerotte @KaraSchmidlin
Thanksgiving looks a lot different this year. Skipping the traditional gathering to keep loved ones safe. We completed time point 14 today and I must say that it was nice to spend a little time with the lab family. #KGSlabEvolves @SamApodaca_ @KSamerotte Happy T-day!
Arguably the least exciting part of this evolution. Labeling cryovials! It’s my turn this week. @SamApodaca_ is up next. #KGSlabEvolves @KSamerotte
When the side project you are working on gets contaminated with bacteria you forget to tweet about time point 13 because you were too busy covering the lab in ethanol to protect your samples!
Don't worry - they are safe. #KGSlabEvolves @SamApodaca_ @KSamerotte
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