Top Tweets for #Subtomogram
Using in-cell #cryoEM & #subtomogram analysis, we determined in-cell maps of yeast, mammalian, & protozoan #NPCs with varying outer rings (CR & NR). A stable basket was found only with the double NR, consisting of struts emanating from the double NR & ending in a distal density.

Our #cryoET study of #RSV is out in @NatureComms!! https://t.co/efSFoABX6S Using #subtomogram averaging we show the matrix protein packs as a helical-like lattice. The Fusion gp arranges as F-trimer pairs & correlate w/the M lattice. Great work @Pangolininator & coauthors!❄️🔬🦠

Once the mechanical load on the nuclear envelope increases during differentiation, nuclear pores are frequently overstretched and disintegrate in Nup133 deficient cells. This remained undiscovered by structural analysis based on #subtomogram averaging.

The highest resolution #Helical, #Subtomogram Averaging & #MicroED entries in #EMDB are the #Tau #Filament (https://t.co/BtrjTFfYLM) @ 1.86 Å, #Apoferritin (https://t.co/4Bfzpbzps7) @ 2.3 Å & #RNA-binding protein FUS (https://t.co/8PZH7uwvw6) @ 0.6 Å, respectively.
#EMDB20

The largest #SingleParticle #CryoEM entry in #EMDB is the #Marine #Siphovirus TW1 (https://t.co/sM2xlHNnqd) at ~12GB! The biggest #Subtomogram Averaging entry is the #Sulfolobus turreted #Icosahedral #Virus bound to Sulfolobus #Pili (https://t.co/XGY6lFKN9K) at ~4GB.
#EMDB20

Available @EMDB_EMPIAR and published in @sciencemagazine, the raw image data that led to the 3.9 Å #cryoET structure of the HIV-1 capsid-SP1 solved using #subtomogram #averaging by @SchurLab, Martin Obr, @WimJHH, William Wan, Arjen Jakobi, Joanna Kirkpatrick, Carsten Sachse…
#inext_ceitec
Florian Schur @SchurLab closes afternoon session with impressive talk about #cryoET reconstruction of #HIV #virion
#StructuralBiology #cryoEM #subtomogram #averaging
@instructhub @CEITEC_Brno @ISTAustria

https://t.co/wOarE4G6Rw 🔬Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows
by
Thorsten Blum
Alexander Hahn
Thomas Meier
Karen Davies
Werner Kühlbrandt
#mitochondria #ATPsynthase #membranecurvature #cryoET #subtomogram #selfassembly
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