Top Tweets for #smFiSH
#Mitochondria researchers listen up:
Stoldt et al. established protocols for #superresolution microscopy of mitochondria, combining #smFISH with #STED and #MINFLUX.
STED-smFISH reveals the spatial relationship of specific mRNAs and proteins, MINFLUX-smFISH shows mRNA folding into distinct shapes and spatial proximity to mitochondrial ribosomes.
https://t.co/oJnAehumw1
🔬 Our latest #Microscopy #AppNote details a method using #smFISH to report the delivery efficiency and knockdown efficacy of an siRNA therapeutic using our Ti2-E inverted microscope and NIS-Elements software with General Analysis 3 (GA3) module: https://t.co/mWmXe3O9ft
🧬 Understanding how drugs work at the molecular level is important for #DrugDevelopment. #smFISH is used to quantify how drugs impact mRNA expression.
🔗 Our #NikonBioImagingLabUSA team has extensive smFISH experience and are ready to assist you: https://t.co/SECj32IcUJ
.@KennethWWitwer et al. propose SPIRFISH, a high-throughput method that combines the #SPIRIS technique with #smFISH for detecting single-particle proteins, RNA, and viruses in extracelluar vesicles. @HopkinsMedicine
#OpenAccess: https://t.co/CkiXcPapvn
🎉 It's #FluorescenceFriday and we are still celebrating #RNADay, celebrated on August 1st. The abbreviation for August is AUG, corresponding to the RNA triplet AUG, the common start codon for protein translation. #smFISH and #MERFISH are #SuperResolution methods used for RNA.
#PALSschool2024
Jokubas Janusauskas - PhD student from @slinnarsson @karolinskainst
is teaching #smFISH with Enhanced ELectric FISH
to inspect the #spatial #Transcriptomics landscape
#python computation at hands!
Jokubas is expert in both Method execution and #computation
#PALSschool2024
Fearless @AnnaNordin96 from my lab @WCMM_at_LiU @liu_universitet
is teaching #transcription factors biology and #peakcalling hands-on computation
Anna is a rising ⭐️ in the field
Today we feature a publication from Prof Melike Lakadamyali from the University of Pennsylvania. Using the #Nanoimager with #SingleMoleculae methods, they show that the regulation of reprogramming is gene-specific. https://t.co/sxKn7uU9Jr
#SMLM #dSTORM #smFISH #OligoSTORM #iPSC
#Baysor
#CellSegmentation for 2D 3D image-based #SpatialTranscriptomics
Based on Transcript composition #MarkovRandomField
-/+Stain image #PriorSegmentationConfidence
Work with #MERFISH #smFISH #STARmap #InSituSequencing
⏫Detection of #EndothelialCell #MuralCell (Fig 5/6)
@PetukhovViktor @KharchenkoLab @NatureBiotech 2022
https://t.co/LaBhk6DQGG
#ScienceJobsTuesday 2: An @FRM_officiel-funded 3-year #Phd position to explore with #scRNAseq and #smFISH of selected informative genes, the natural variation in quantitative gene expression profiles across individuals of a given species and between species.
Oyez, Oyez, we are seeking to expand our team by recruiting 3 new colleagues, #PhDstudents and #postdoc. If you love #LiveImaging, #lightsheet, #scRNAseq, #smFISH, #QuantitativeBiology, #ascidians, #embryos and, please read on (and RT) 👇
#sciencejobs @SFBD @ISDB @TLM @SMBdevBio
It is tomorrow at 1PM EST. #RNAISH #smFISH #scRNAseq #singlecelltranscriptomics #singlecell #singlecellRNA #RNAscope
Did you know #RNAscope is a quantitative assay (each puncta dot is one copy of a RNA mol) and you can use imaging software like Halo from Indica Labs to do the analysis. To learn more do register for the webinar below.
Join us Today at 1 pm Science building G19 , NYU for an engaging lunch and learn session with Cynthia Loomis @NYUmedical and Connie Zhang @ACDbio to learn about the versatility of RNAscope assays: https://t.co/O6j2PJYKdZ #singlecell #smFiSH
A small sneak-peak at how buffering of biological noise helps reproduce tissue patterns faithfully during development.
#DrosophilaEye #smFISH #EGFRSignaling
🪰 Check out this recent #Drosophila eye study from researchers at @TIFRH_buzz: https://t.co/lLu50vFL1W
New research in #Tuberculosis! we use #smFISH and its derivatives to image immune markers in #rabbit and #macaque granulomas, paving the way for a better understanding of the disease. Read more at https://t.co/fTXq7VPGVx #TuberculosisResearch #Immunology
From my colleague @AllisonRossol, a smart use of Chat GPT - to craft haikus!!
With #RNascope's sharp hold.
Insight, clarity it brings,
To cell therapy's secret things.
#ThisISHworks #RNAISH #cellandgenetherapy #singlecelltranscriptomics #smFISH #scRNAseq #spatialbiology
2nd paper this year from our lab. Congratulations to @KemalKeseroglu and @OrianaZinani for publishing protocol paper on using single-molecule fluorescence in situ hybridization (#smFISH) and immunohistochemistry to count molecules in single cells in @ZebrafishRock
I am delighted to share our new protocol paper on using single-molecule fluorescence in situ hybridization (#smFISH) and immunohistochemistry (#IHC) to count RNA molecules in single cells in #zebrafish embryos has been published in @STARProtocols
I am delighted to share our new protocol paper on using single-molecule fluorescence in situ hybridization (#smFISH) and immunohistochemistry (#IHC) to count RNA molecules in single cells in #zebrafish embryos has been published in @STARProtocols
I am in love with my 🦇 #smFISH results showing the small intestinal villi and crypts of a nectar #bat! nuclei in yellow, glucose transporters in pink and cyan @HCRimaging🔬#sciart #bioart #FluorescenceFriday #Microscopy #GITwitter #histophysiology #diet
Researchers characterize the life cycle of individual parasites and their hepatocyte hosts throughout the #Plasmodium liver stage!
https://t.co/4db32FIQYq
They identified potential adaptive programs and zonal trends in both the host and the parasite.
#scRNAseq #smFISH
Why does sensitivity matter in detecting transcripts in tissue samples? This image shows the sensitivity comparison between #scRNAseq, #insitu #sequencing and #smfish. Each dot represents one transcript.
#singlecell #genomics #omics #spatialbiology
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