Jonathan Stefely, MD, PhD

physician-scientist studying fundamental mechanisms of biology in infectious amoeba.

[Jonathan Stefely, MD, PhD @jastefely]

Our paper "Mitochondrial proteome of Acanthamoeba delineates aerobic and anaerobic pathways dynamically regulated by oxygen" is online now @CellCellPress! https://t.co/NPjdwdwcHD. Grateful to my amazing post-doc mentor @VamsiMootha and all the other team members!

[Alice Ting @aliceyting]

Remarkable Magnum Opus from @VamsiMootha & the Mitocarta Tree of Life consortium consisting of 9 back to back research papers (+2 perspectives) just released in Cell and Cell family journals:

- Characterization of mito proteomes from 6 diverse species, from Giardia to Leishmania to Arabidopsis
- Optimized methods for mito enrichment, DIA mass spec, protein correlation profiling, microscopy validation, and cryoEM analysis of native complexes
- Comparative analysis reveals dozens of proteins shared among pathogens but lost in human hosts = “pan-parasite” drug targets
- Data used to re-train ML predictor of subcellular localization => predicted mitoproteomes across hundreds of eukaryotes
- Giardia solute carrier discovered with functional validation
- Dynamic rewiring of Acanthamoeba mito proteome from aerobic to anaerobic energy production characterized

And tons more… The papers are instant landmarks and raise even more questions than they answer, paving the way for many future investigations of this fascinating organelle #mitochondria

https://t.co/yjUnnh6CI3

[Genetic Engineering & Biotechnology News @GENbio]

Proteomics Maps Eye-Infecting Acanthamoeba Mitochondria Across Oxygen Levels

The Acanthamoeba mitoproteome was mapped, revealing oxygen-driven metabolic shifts and potential targets for safer infection treatments
@jastefely @VamsiMootha
#proteomics
https://t.co/vYy3Ujo7pX

[Cell @CellCellPress]

Our new issue features the MitoCarta Tree of Life (MitoTOL) Consortium👉https://t.co/uP2kI6DjtL

On the cover: a transmission electron micrograph of mitochondria , digitally rendered in the style of a watercolor painting.

[Nathaniel David @NathanielEDavid]

I (fondly) remember as a TA insisting Berkeley undergrads memorize the Krebs cycle and OxPhos ('it's good for your soul,' id say). We'd draw the Krebs cycle as a loop floating free in the mitochondrion, and the respiratory chain as a separate machine in the membrane. In one organism they are bolted together.@VamsiMootha

https://t.co/8vjO6r2RZz

[Michael Zhu Chen @michaelzhuchen]

Proud to share the fruits of a 4 year effort with an amazing team at the MitoCarta Tree of Life Consortium! Today, we report insights on the origin and evolution of mitochondrial proteomes across billions of years: https://t.co/3iaZWTejSS
https://t.co/eQCeNUqotj

[Jonathan Stefely, MD, PhD @jastefely]

The @Morgridge_Inst for Research is hiring a new metabolism-focused Investigator! Joint tenure-track at UW-Madison, outstanding colleagues, support, facilities, and resources! Apply to join us in Madison! https://t.co/vabuYjq4xC

[Jonathan Stefely, MD, PhD @jastefely]

@EdreesRashan @CellCellPress @VamsiMootha Thanks, Eddie! It was fun to overlap in Boston for a few years!

[Jonathan Stefely, MD, PhD @jastefely]

Our paper "Mitochondrial proteome of Acanthamoeba delineates aerobic and anaerobic pathways dynamically regulated by oxygen" is online now @CellCellPress! https://t.co/NPjdwdwcHD. Grateful to my amazing post-doc mentor @VamsiMootha and all the other team members!

[Jonathan Stefely, MD, PhD @jastefely]

Our paper on a new genome annotation for the pathogen Acanthamoeba castellanii is now online at @cellpressblue! https://t.co/09a4u5Dbm4. This was a key foundation for our separate AcMitoCarta paper. Grateful to John Samuelson and @VamsiMootha for their mentorship!

[Vamsi Mootha @VamsiMootha]

Today, the MitoCarta Tree of Life Consortium (MitoTOL) is releasing high-accuracy mitochondrial proteomes across diverse eukaryotes to empower studies of physiology, evolution, and disease:

https://t.co/hRt6ZG4lQP

All data can be accessed at:

https://t.co/oPFODZBQ60

[NEJM @NEJM]

In a 28-year-old woman with postpartum immune thrombotic thrombocytopenic purpura, plasma exchange was unsuccessful. Near death while receiving vasopressors, she was treated with recombinant ADAMTS13, and the disease abated. Read the Brief Report: https://t.co/rhdVbWFcnA

[Jonathan Stefely, MD, PhD @jastefely]

Recombinant ADAMTS13 for Immune Thrombotic Thrombocytopenic Purpura | New England Journal of Medicine https://t.co/7jNvxDws8I @MGHPathology Blood Transfusion Service

[Rahul Gupta @rahulg603]

Out today @Nature – our analysis of the influences of common nuclear DNA variation on mitochondrial DNA copy number and heteroplasmy across >250,000 people across two biobanks! https://t.co/VjYsSE0BOg

[Jonathan Stefely, MD, PhD @jastefely]

@MGHPathology Grateful to be a member of the @MGHPathology team! Awesome first two months as a transfusion fellow—kicked off with fellow @UWMSTP alum @AmeliaHaj, MGH BTS attending Patty Brunker, and heme/onc fellow Christine Ryan, among others!

[Jonathan Stefely, MD, PhD @jastefely]

Blood clots can develop in patients with #COVID19, but the mechanisms are unclear. Could we predict patients at high risk? We started to learn more about this while working on the @MGHPathology coagulation service during the pandemic peak: https://t.co/eFDh2hFMjc @MassGeneralNews

[Jonathan Stefely, MD, PhD @jastefely]

@MGHPathology @MassGeneralNews @tasillas @DrWRothwell Excited to kick off another year with this team @MGHPathology @tasillas @DrWRothwell, Cynthia, Megan, Kristie, and @adamsfisch!

[NEJM CPC @NEJMCPC]

Dr. @katrinarmstrong, Chief of @MGHMedicine at @MassGeneralNews, expertly discusses the differential dx for this week's @NEJM #CaseRecords:⁣
Case 16-2020: 48W w/ Recurrent #Melanoma & Pulm Nodules.
⁣
Congrats Drs. Armstrong, @DrJustineCohen, Shepard, Folch, Mansour, @jastefely!

[Jonathan Stefely, MD, PhD @jastefely]

@NEJMCPC @katrinarmstrong @MGHMedicine @MassGeneralNews @NEJM @DrJustineCohen @MassGeneralMDs @MGHImaging @MGHPathology @MGHCancerCenter @mgh_id Grateful for the opportunity to be a part of this team! @MGHPathology @MGHMedicine @MassGeneralNews @mgh_id

[Pagliarini Lab @Pagliarini_Lab]

After 10+ fantastic years in Madison, it is bittersweet to announce that my lab and I are moving to @WUSTLmed soon. I am so grateful to everyone at @UWBiochem and @Morgridge_Inst for their support and guidance. Stay healthy, and come visit us in St. Louis!
https://t.co/zA6bauAEgz

[Pagliarini Lab @Pagliarini_Lab]

Very proud of lab alumna Danielle Lohman, an amazing biochemist now applying her skills to US science policy. Check out this @cenmag article about Danielle and other recent PhDs now engaged in #scipol. https://t.co/1RhIOEfNZP