Our paper "Memory reactivation generates new, adaptive behaviours that reach beyond direct experience" is now out https://t.co/oiTC4HH2ks ! With @AnnieBRawson, Jill OโReilly and @HelenCBarron at @MRCBNDU@NDCNOxford 1/6
Ever wanted to explore the driving forces underlying inhibitory neuronal signalling? We did! So, for my MSc in @JosephRaimondo lab, we developed the all-optical approach ORCHID - all-Optical Reporting of CHloride Ion Driving force. Now out in @NatureComms https://t.co/kJGv5mZkpg
ORCHID is additionally advantageous in small cells or subcellular compartments, and opens the doors for using all-optical methods to assess driving force more generally. (5/7)
We use ORCHID to confirm the mechanisms that establish Cl- driving force, reveal novel differences between neurons and astrocytes under different network states (1), and make the first in vivo measurements of intact Cl- driving force in mouse cortical neurons (2). (4/7)
We are excited to present ORCHID, a new tool for all-Optical Reporting of Chloride Ion Driving force. https://t.co/HdKWAazf31 In the brain, inhibition is mediated by Cl- permeable receptors. ORCHID can therefore be used to quantify the driving force across inhibitory receptors.