Josh Selfe

Neuroscience PhD student in the Akerman lab @OxNeuro. Intrigued by cortical circuits, all-optical techniques, sensory processing.

[Josh Selfe @joshsselfe]

@SumedhaNalluru @AnnieBRawson @HelenCBarron @MRCBNDU @NDCNOxford amazing work!

[Sumedha Nalluru @SumedhaNalluru]

Our paper "Memory reactivation generates new, adaptive behaviours that reach beyond direct experience" is now out https://t.co/oiTC4HH2ks ! With @AnnieBRawson, Jill O’Reilly and @HelenCBarron at @MRCBNDU @NDCNOxford 1/6

[Josh Selfe @joshsselfe]

@kiradusterwald Thanks for the awesome modelling Kira and @EranShorer!

[Josh Selfe @joshsselfe]

@RichBur @JosephRaimondo Thanks Rich!

[Josh Selfe @joshsselfe]

@ewadorotaz Thank you Ewa! :)

[Josh Selfe @joshsselfe]

@_anngru_ Thanks Anna! :D

[Josh Selfe @joshsselfe]

Huge thanks to @Abdelfattah_Lab, @ERSchreiter, and @rhodamine110 at @HHMIJanelia, and to @RichBur, Sarah Newey, and Colin Akerman at @PharmOxford for all of the help and support along the way! And to @MPrag for the photo of the beautiful orchid 📷 (7/7)

[Josh Selfe @joshsselfe]

Ever wanted to explore the driving forces underlying inhibitory neuronal signalling? We did! So, for my MSc in @JosephRaimondo lab, we developed the all-optical approach ORCHID - all-Optical Reporting of CHloride Ion Driving force. Now out in @NatureComms https://t.co/kJGv5mZkpg

[Josh Selfe @joshsselfe]

snRNAseq by Teresa Steyn, computational modelling by @EranShorer and @KiraDusterwald, and IHC by @AKraitzick. All with @UCT_HUB, @UCT_NI, @UCTIDM, and @UCTHealthSci. (6/7)

[Josh Selfe @joshsselfe]

ORCHID is additionally advantageous in small cells or subcellular compartments, and opens the doors for using all-optical methods to assess driving force more generally. (5/7)

[Josh Selfe @joshsselfe]

We use ORCHID to confirm the mechanisms that establish Cl- driving force, reveal novel differences between neurons and astrocytes under different network states (1), and make the first in vivo measurements of intact Cl- driving force in mouse cortical neurons (2). (4/7)

[Josh Selfe @joshsselfe]

Amazing work by @gsgothard showing the developmental origins of the local inhibitory subnetworks of L2/3 pyramidal neurons. Give it a read!

[Josh Selfe @joshsselfe]

@Carlos_Aplysia Thank you Carlos!

[Josh Selfe @joshsselfe]

Super excited to share that this preprint is now out, the result of my MSc, and the first all-optical reporter of ionic driving force!

[Joseph Raimondo @JosephRaimondo]

We are excited to present ORCHID, a new tool for all-Optical Reporting of Chloride Ion Driving force. https://t.co/HdKWAazf31 In the brain, inhibition is mediated by Cl- permeable receptors. ORCHID can therefore be used to quantify the driving force across inhibitory receptors.