Mark Farrant

Professor of Neuroscience UCL

[Ian Coombs @IanDCoombs]

Our first feelgood foray into translational research: Perampanel can be therapeutic for patients with overactive GRIA variants.
Half the length of the first submission, but I still like it and it takes less time to read... @CureGrin @JulieZiobro @uclnpp
https://t.co/52zoj0vQ17

[Mark Farrant @m_farrant]

@royalsociety Congratulations Tiago!! @trabranco Excellent news to brighten my day.

[Mark Farrant @m_farrant]

@Annalisa4fun Honorary? Hah!
I was wondering what might be in there after all these years... but then I couldn't help admiring the correctly used apostrohe...

[Mark Farrant @m_farrant]

Just out – our new paper on the actions of the TARP γ8-selective AMPAR blocker JNJ-55511118. Great work from (the far speedier tweeter) @IanDCoombs and Craig Sexton @uclnpp.

https://t.co/6ol4DAWi1h

[Mark Farrant @m_farrant]

Just posted @medRxivpreprint – the use of perampanel in a patient with a #GRIA2 gain-of-function variant. Enormous thanks to the patient and family, and to @JulieZiobro and @CureGRIN. All driven by the excellent @IanDCoombs @uclnpp.

https://t.co/VHErtO9CEs

[bioRxiv Neuroscience @biorxiv_neursci]

Extrasynaptic GABA waves underlie epileptiform rhythms in the hippocampal network https://t.co/FiuVEM20Cv #biorxiv_neursci

[Daniel Choquet @daniel_choquet1]

The pre-print of our new paper "Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids allows access to masked epitopes in live neurons" https://t.co/78UfzDNPJs @iins_bordeaux @LabSauer @INSB_CNRS @univbordeaux @biorxiv_neursci @Neuro_Bordeaux

[Mark Farrant @m_farrant]

Time to catch up on some AMPAR reading... two elegant studies from @AndrewPlested and colleagues:

https://t.co/YhOG78F1Bc

and

https://t.co/y6K1CgY8lP

[Mark Farrant @m_farrant]

3/3 For mEPSCs in neurons cultured from GluA2 KO mice, NASPM, unlike spermine, blocked all outward currents. Thus, NASPM-dependent full rectification offers a clear advantage over use of spermine and should allow a more quantitative assessment of the CP-AMPAR content of synapses.

[Mark Farrant @m_farrant]

2/3 Starting from the question “Can we do better than 100 μM intracellular spermine?”, we examined other blockers and found that 100 μM intracellular NASPM produces complete block of outward currents through CP-AMPARs – regardless of their co-assembly with auxiliary subunits.

[Mark Farrant @m_farrant]

1/3 New at @biorxiv_neursci, our @IanDCoombs-led study of CP-AMPAR block.
https://t.co/fwvNQpcaKK

Spermine-dependent rectification is widely used to reveal CP-AMPARs, but auxiliary proteins alter spermine block/permeation, limiting its utility as a tool for their detection.

[Mark Farrant @m_farrant]

@Schlab4 @affinitybyserif Yes a beta. Will instal alongside your purchased version (not overwrite it).

For Mac see https://t.co/houRBIFgZS

[Mark Farrant @m_farrant]

When those figures just need those final adjustments...

At last, the missing 'Select Same' magic that kept me going back to Illustrator comes to Affinity Designer from @affinitybyserif.

[Mark Farrant @m_farrant]

Fascinating new study from the Greger Lab @MRC_LMB –

Gating transitions and modulation of a hetero-octameric AMPA glutamate receptor

"...GluA1/2 receptor assembled with both TARP-γ8 and CNIH2... in both resting and active states..."

https://t.co/xTfHWcYsNy

[Mark Farrant @m_farrant]

We have a #postdoc position available in the Farrant/Cull-Candy lab @uclnpp to study AMPAR function and regulation by auxiliary proteins. Please RT. Details here: https://t.co/dYU0VeYcO4

#neuroscience
#ionchannel
#electrophysiology
#molecularbiology

[Mark Farrant @m_farrant]

Fascinating new study on Shisa7 by Wei Lu and colleagues.

Han et al - Shisa7 is a GABAA receptor auxiliary subunit controlling benzodiazepine actions

"...a new dimension for understanding and reanalysis of GABAAR function and pharmacology"

https://t.co/HDlcBoR6H7

[Mark Farrant @m_farrant]

Just out @NatureComms, our first @biorxiv_neursci submission makes it into 'print' - https://t.co/CmSDiIVjkU. Congrats to @IanDCoombs and all past and present @uclnpp concerned.

[Mark Farrant @m_farrant]

New online at @biorxiv_neursci, our @uclnpp study of homomeric Q/R edited AMPA-type glutamate receptors: desensitized but not closed! Down to the hard work and persistence of @IanDCoombs.

https://t.co/vasfIW1Daa

[Mark Farrant @m_farrant]

Now online at @SfNJournals eNeuro Early Release, our @uclnpp/@UCL_ICH study on AMPA-type glutamate receptors in a mouse model of juvenile #BattenDisease. All thanks to the hard work of DS.
https://t.co/8O64iDHgZd

[Mark Farrant @m_farrant]

Out in print @CellReports – fruits of nice @uclnpp collaboration with Kittler lab! Clustering of GABAA-Rs by LHFPL4.
https://t.co/Wx38j0aBYF