michael hudson

[michael hudson @mejhudson]

@hsalis I think they're either using bacteria to do nick repair, but they do mention use of PNK in one section of the methods:
"The ligation reaction (20 μl total volume) consisted of 17 μl assembled fragments, 2 μl of 10× T4 DNA ligase buffer and 1 μl polynucleotide kinase."

[michael hudson @mejhudson]

@ATinyGreenCell Gotcha. I've had them make ultramers with 20nt at 50%gc and then 100T in a row for adding polyA tails to mRNA templates, but agree, not sure about 0% GC.

[michael hudson @mejhudson]

@ATinyGreenCell Could Ansa do these?

[World War II History @WW2Facts]

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[michael hudson @mejhudson]

@ATinyGreenCell Could this possibly be fractal crystal growth of some sorts? Some non-biological process might make something that looks like that?

[michael hudson @mejhudson]

@ATinyGreenCell That would depend if there is a cryptic promoter upstream I guess. I'm assuming that atg is part of the -10 region of the promoter, so probably don't have any transcription from that point.

[michael hudson @mejhudson]

@ATinyGreenCell There is an atg upstream, is that in the other strength promoter versions? Any chance this one is making a small peptide that is toxic? The deletion is removing a stop codon in frame to the atg in the promoter.

[michael hudson @mejhudson]

@ATinyGreenCell @liv4bacteria I have a nice lab in the Boston biotech industry, but that's a really inviting lab. Super nice place to work. Really jealous!

[michael hudson @mejhudson]

@ATinyGreenCell What's in the 0, 200, 300, 500 tubes? Volume balances for centrifuge?

[michael hudson @mejhudson]

@mr_albright_ @MassHSHockey @MassNZ @T_Mulherin @HNIBonline @GlobeSchools @MetroWestSports @NEHockeyJournal @MHLbbiglive @HockeyNightInNE @BGlobeSports Good to see the tradition of good sportsmanship is alive and well at Weymouth high school!
@WHhockey1, @CoachKennedyWHS

[Mark Albright @mr_albright_]

So nice they had to win it twice!
3-2 OT vs Weymouth.

Noah gets go-ahead w/ 33 sec left. Signaled goal but they pull the puck out & convince refs to change call.

Proud the boys didn’t let the “no goal” change their approach and came out with a win. #goflyers

[michael hudson @mejhudson]

@ACSSynBio Link is still broken!

[michael hudson @mejhudson]

@ATinyGreenCell Thanks! Will give it a try.

[michael hudson @mejhudson]

@ATinyGreenCell Do you have a link to the gel purification protocol you used here? I'd be interested in trying it.

[michael hudson @mejhudson]

@breakliquid Problem of wanting to use thin walled plastic to get better heat conduction, but makes them flimsy. I solve it by putting a strip of tubes at each side of the well block. Decreases pressure on your tubes.

[michael hudson @mejhudson]

@VerizonSupport @comcast @astoundconnects Big thank you to @astoundconnects for taking care of this. Much appreciated!

[michael hudson @mejhudson]

@VerizonSupport @comcast @astoundconnects Not sure whose wires these are, but they are hanging into the road. Already seen them snagged by the garbage trucks. Any chance someone knows who to contact to get this fixed?

[michael hudson @mejhudson]

@davidycli @threadreaderapp unroll

[David Li @davidycli]

If I could go back in time to start a biotech,

#1 change I'd make would be -->