A great pleasure for me and Angel Angelov (https://t.co/xyb7wqEWQg) giving guest lectures for @Mo_Li_KAUST's Introduction to Nanopore Sequencing course @nanopore@KAUSTBCL @kaust_corelabs
We assembled the genome of an individual from Saudi Arabia and make the assembly and sequencing data freely available; the genome is almost complete (compared to CHM13) and fully annotated; great collaboration with @MalakAbed @kaust_corelabs @CBRC_KAUST
https://t.co/0hwPy98Nw0
My friends, a new MEGATHREAD has arrived!
In 40 tweets I’ll explain 40 useful concepts you should know.
Reading time: ~7 minutes.
Value: potentially a lifetime!
Thread:
Welcome to our newest Center leader, Dr Francesca Short. Fran is interested in the regulatory networks that govern virulence and antimicrobial resistance in gram-negative pathogens & molecular mechanisms of antimicrobial biocides. Fran is helping drive our OneHealth agenda!
At the upcoming @NanoporeConf community meetings I will present a new, @nanopore only lettuce reference genome. Highly contiguous and highly accurate. https://t.co/k9VKNi3a3T
The genome sequence of the Australian filarial nematode, Cercopithifilaria.... McCann K et al., published by @WellcomeOpenRes, https://t.co/CYTVdumhVH
A nice outcome from a side project, a long long time ago in the @lab_grant at @PAM_latrobe . Very happy for @Kirsty_m_McCann 🙌
Looking forward to run these brand new @nanopore R10.4 cells @KeyGeneInfo Our aim is to increase accuracy further using trained models and increase the number of duplex reads. Q20+ #ngs#longreads#crops#breeding
Excited to see our paper out, and much improved from the pre-print version - we find that the “real job” of AmvA, a disinfectant resistance efflux pump from A. baumannii, is transport of polyamine metabolites. https://t.co/GHmJ39SAKO
gggenomes - an R package for comparative genomics, which extends the ggplot2 by adding dedicated plot functions for genes, syntenic regions, and verbs to manipulate the plot.
https://t.co/RiosjLUUq6
#rstats#genomics#DataVisualization#ggplot2
Less than one week left (closes 19th September) to apply for the postdoctoral researcher position in my group to develop and lead single-cell genomics for helminth parasites.
Will suit someone with good molecular biology skills with an interest in bioinformatics.
@GenomeBiology@mrdoods@PacBio Our optimized workflow covers sample collection, DNA extraction, library prep, QC & genome assembly. Extras: DNA extraction protocol https://t.co/rUJwXlRReX; ONT, CLR & HiFi sequencing; HiFi vs high N50 CLR assembly; evaluating 4 HiFi assemblers & loads of stats + supp. info 2/2
@GenomeBiology@mrdoods Our LeafGo workflow has been published. From leaves to high-quality genomes ≥ 7 days. Extracted HMW DNA from 10 different plant species, sequenced and assembled only with @PacBio#hifi#hifiasm➡️ 2 eucalyptus + polyploid peanut genomes (N50 > 36Mb)
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I am excited to announce I am looking for a postdoctoral fellow to develop and lead single-cell genomics on helminth parasites for the next 3 yrs. Will be a combination of wet and dry lab. Please RT and share! https://t.co/jeneDQvXbc