Prashant Mali

[Prashant Mali @prashantgmali]

@kalhorlab @CellCellPress @JEFworks very cool! congrats Reza and team!

[Nature Biotechnology @NatureBiotech]

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells https://t.co/KylFX25wjK

[Prashant Mali @prashantgmali]

@harriswangnyc @ColumbiaSysBio @ColumbiaBME @Columbia so cool! congrats Harris and team!

[Prashant Mali @prashantgmali]

@exosome Thanks!

[Prashant Mali @prashantgmali]

New pre-print from the lab!

Customizing AAVs is usually a slow, trial-and-error process. Leveraging mosaic AAVs, Nolan’s new paper presents MATCH, a "plug-and-play" toolkit that clicks homing proteins directly onto the virus.
>80x better brain transduction
>50% transduction of resting PMBCs
Simple one-pot production

full paper: Receptor-guided AAV Tropism Engineering via MATCH https://t.co/A5KEdVUPr7

[Prashant Mali @prashantgmali]

@srikosuri @rhomsany Awesome -- congrats Sri!

[Prashant Mali @prashantgmali]

@harriswangnyc @Columbia @ColumbiaMed @ColumbiaSysBio Congrats, Harris!

[Prashant Mali @prashantgmali]

@Richard_She Indeed, Richard — the choice of linker length and display stalk play a critical role in ensuring signaling responses. There are many applications to explore, and as you noted too, we’re especially excited about the possibilities for combinatorial screening.

[Prashant Mali @prashantgmali]

Pooled screening of secreted factors (cytokines, growth factors) is difficult due to paracrine cross-talk.

By genetically tethering ligands to the cell surface to enforce obligate autocrine signaling, OASIS enabled a pooled screen of the entire human ligandome (~770 factors) in iPSCs + single-cell Perturb-Seq.

https://t.co/SNngfPXuXy

[Prashant Mali @prashantgmali]

@anideshpandelab 🙏

[Prashant Mali @prashantgmali]

Regulatable gene expression via endogenous ADAR modulatable Aptamers (Adaptamers).

The system is a compact (<120 bp) cis-regulatory RNA switch. Ligand binding stabilizes a dsRNA motif, recruiting ADAR to edit a premature Stop codon ➔ Sense codon, restoring translation.

• Mechanism: Post-transcriptional, cleavage-free, Stop-to-Sense editing.
• Payload: No exogenous protein regulators required (minimizing immunogenicity/size).
• Validation: Functional in human T-cells; AAV-delivered FGF21 control reversed obesity in mice.

https://t.co/E7rZAp2Y4C

[Prashant Mali @prashantgmali]

New preprint from the lab led by the amazing Alex Lee, Yesh Doctor, and Yifan Zhang! # high-throughput screening of secreted proteins
"Multiplexed Pan Soluble Ligandome Assaying via OASIS" https://t.co/olPxOIBPtY

[Prashant Mali @prashantgmali]

New preprint from the lab led by the amazing Jack Bryant!
# tunable genetic medicines, # endogenous ADARs
"Regulatable In Vivo Gene Expression via Adaptamers" https://t.co/214uU0ckxG

[Prashant Mali @prashantgmali]

3rd generation guide RNA designs for programmable RNA editing via endogenous ADARs https://t.co/YQ2u2dZjtf : work led by the amazing Susan Byrne and Adrian Briggs!

[Prashant Mali @prashantgmali]

@broadinstitute @davidrliu @brkthroughprize congrats @davidrliu ! wonderful news!

[Prashant Mali @prashantgmali]

@srikosuri ❤️

[Prashant Mali @prashantgmali]

@HowardYChang @Amgen Congrats Howard!

[Elliot Hershberg @ElliotHershberg]

This is a great resource!

Preprint:
https://t.co/VsgaSLtRTT

[Michael S. Breen @breenPsychGene]

#ADAR and #RNAediting in brain organoids (as neural specific) and teratomas (as pan-tissue) developmental models.