Adrian G. R.

MSc candidate in Medicinal Chemistry @ University of Copenhagen | Jesper L. Kristensen lab | @[email protected]

[Adrian G. R. @softnucleophile]

@ndchiappini @GracePanetti Phen + menthol is my go-to for most organolithium/grignard tbh, but what do you mean by "it only does total base"? As in, e.g. hydroxide impurities will interfere, or am I misunderstanding?

[Adrian G. R. @softnucleophile]

@madras_bertha @zenbrainest There are washing steps prior to ligand addition, so one can argue that the 5-HT will mostly be gone. I think the main worry would be receptor desensitization/internalization induced by high [5-HT].

[Adrian G. R. @softnucleophile]

@zenbrainest Yeah, that's a weird "control" experiment. Should have compared it to dialyzed serum.

[Adrian G. R. @softnucleophile]

@Colemanomonas @ATinyGreenCell Ofc depends on desired info, but Isothermal Titration Calorimetry (ITC) is nice and label-free, tho data analysis can sometimes be less trivial. If you just want to know how much binds, then dialysis with MW cut-off between mRNA and protein might work similar to your proposal.

[Adrian G. R. @softnucleophile]

@ndchiappini Thanks! Maybe I'll buy some if EU shipping isn't super expensive.

[Adrian G. R. @softnucleophile]

@ndchiappini Are those aluminum granules I see? Where did you get them? I really wanted to buy some Lab Armor alu beads for heat baths, but they're ridiculously priced, and the bulk aluminum granules I bought were too fine and rough to be useful.

[Adrian G. R. @softnucleophile]

@KRHornberger Best solution I found for us who use Firefox (works on a lot of publisher sites): https://t.co/rKC73jFOB8

[Adrian G. R. @softnucleophile]

@MoleculesGoHard @AmerChemSociety Tip: Use extension "Redirector" (@einaregilsson) in Firefox/Chrome, and add rule(s) to make it redirect epdf to pdf. Rule below/in pic might only work for certain sites, but works for ACS journals atm.

RegEx pattern: ^(.*)/doi/epdf/(.*)
Redir to: $1/doi/pdf/$2

[Adrian G. R. @softnucleophile]

@Robert_Palgrave There's a decent comment on the chemistry at PubPeer too (https://t.co/k92LuY0Qga). I'm no solid state chemist, but their experimentals and characterization seem pretty dodgy tbh. Obv just a preprint, but I'm very doubtful. Extraordinary claims, extraordinary evidence, etc.

[Adrian G. R. @softnucleophile]

@MoleculesGoHard @AmerChemSociety Tip: Use extension "Redirector" (@einaregilsson) in Firefox/Chrome, and add rule(s) to make it redirect epdf to pdf. Rule below/in pic might only work for certain sites, but works for ACS journals atm.

RegEx pattern: ^(.*)/doi/epdf/(.*)
Redir to: $1/doi/pdf/$2

[Adrian G. R. @softnucleophile]

@JImmatSci So the same team has observed phosphine again? Is that it? I remember people questioning quite a bit whether any kind of life is really even necessary for phosphine gas production. Anyone, please enlighten me.

[Adrian G. R. @softnucleophile]

@BagPhos @AmerChemSociety I am appalled.

[Adrian G. R. @softnucleophile]

@chem_alexrob @Chemjobber I'm a bit surprised at people's response, I have to admit. I don't think I've ever cleaned an NMR tube for reuse.

[Adrian G. R. @softnucleophile]

@zenbrainest @sequencemyneuro That's something the research community should aim to change, though. I know it's difficult to get funding for such, but if one has a little bit of extra resources, just pre-print publish it. Don't really have to prove a negative, just that it wasn't able to be replicated.

[Adrian G. R. @softnucleophile]

@SamesLab @scisignal Cool paper, but doesn't really refute much? While I'm not entirely convinced by the Castrén article, it only claims positive allosteric modulation, not "direct activation". Am I missing something?

[Adrian G. R. @softnucleophile]

@Maxime_R_Vitale @atkim3 Would bet 15-40 μm or thereabouts – at least that's the typical particle size ime.

[Adrian G. R. @softnucleophile]

@radscientist_ Fair enough, all our hotplates look like hot garbage anyway lol

[Adrian G. R. @softnucleophile]

@Explosions_Fire Like, adding my NMR spectra as SVG made it more than triple in file size and now I have to wait for like 5 min each time it autosaves

[Adrian G. R. @softnucleophile]

@Explosions_Fire Currently writing my MSc thesis in Word, and god damn it makes me wish I had just started out with LaTeX smh

[Adrian G. R. @softnucleophile]

@ndchiappini I bet it's because OsO4 is sometimes used to stain tissue for TEM (iirc), and bio ppl sometimes have powdered milk for stuff like making blocking solutions for ELISA or something