Srich Murugesan

Cell biologist & microscopist @Zeiss_micro. Formerly postdoc @NIH, PhD @Cornell, BS @iubloomington. 🇮🇳-🇭🇺-🇳🇱-🇿🇲-🇺🇸 🔬

[Srich Murugesan @srich7]

Anyone who has ever done microscopy ever™️

zzz
　＜⌒／ヽ-､_＿_
／＜_/＿＿＿＿／
￣￣￣￣￣￣￣

　　　∧_∧ i didn’t turn off
　　 (　･ω･) the lasers
　 ＿|　⊃／(＿＿_
／　└-(＿＿＿_／
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[HHMI | Janelia @HHMIJanelia]

🎙️Janelia’s Gerry Rubin talked to NPR about the fly brain connectome, how it could help scientists begin to compare the brain wiring of different species & what’s next for connectomics and neuroscience.🧠

https://t.co/NE3jMauCZS

[Srich Murugesan @srich7]

Loved this episode- kudos to @KedarNarayan2 @BoSoxBioBeth and Jason for openly sharing their journeys 🧠 🔬

[Peter O'Toole @YorkBioimaging]

Brave and important. A #mentalhealth podcast special. Hear first hand experiences from two hugely successful scientists, Beth Cimini and Kedar Narayan. Whilst a serious issue, it is very much a relaxed, engaging chat.
See/Hear - https://t.co/dEFlykGnHk
#TheMicroscopists
Pls retweet

[Srich Murugesan @srich7]

Look at those gorgeous actin dynamics!!

[Jesse Rudd-Schmidt @Jspotsounds]

It was great to present at the @PeterMacRes Tech day this week, showing off the capabilities of @CahmPmcc 's recently installed @zeiss_micro Lattice Light Sheet 7 microscope!

Time-lapse imaging of tumour cells expressing a fluorescent actin binding peptide, Lifeact-GFP 👇🏻

[Africa Microscopy Initiative @AMI_microscopy]

📢GOOD NEWS!
The Africa Microscopy Initiative, in partnership with @zeiss_micro will distribute refurbished research-grade light microscopes to African Scientists.
Read more and APPLY: https://t.co/WLcJckSCPE
Deadline: 13 September 2024

[Nature Methods @naturemethods]

This month's Focus on advanced AI in biology also contains a series of Comments from leaders in their respective fields, who discuss the broad impacts that AI-based methods are likely to have on many areas of biological research.

[Srich Murugesan @srich7]

Back at the old stomping grounds #Cornell #ithaca #gorges

[Patrick Hsu @pdhsu]

What if we could universally recombine, insert, delete, or invert any two pieces of DNA?

In back-to-back @Nature papers, we report the discovery of bridge RNAs and 3 atomic structures of the first natural RNA-guided recombinase - a new mechanism for programmable genome design

[Srich Murugesan @srich7]

TIRF-SIM was a game changer for us to figure out actin dynamics in T cells. Big thanks to Dong Li, @shaomumu and @Eric_Betzig- I was very lucky to work with you all at Janelia. Now who can do this in 3D in the context of a true T cell synapse??! #FluorescenceFriday

[Eric Betzig @Eric_Betzig]

#FluorescenceFriday #cellbiology #immunology
#Actin dynamics at the immunological synapse (IS) between a Jurkat T cell and an antigen-presenting coverslip, as seen by total internal reflection structured illumination microscopy (TIRF-SIM). John Hammer's group #NIH used our TIRF-SIM to show that formins rearrange inward flowing actin filaments from the outer lamellipodial band of the IS into circumferential arcs that establish the peripheral supramolecular activation cluster, which in turn concentrates T cell receptor microclusters at the center of the IS. https://t.co/XuFawsimD6

[Joey Tidei @J__Today]

Life has been pulling me away from the scope (I had a baby named Henry!), but #FlourescenceFriday never takes a break. CAD cell with a dual tag myosin 2 construct - green on the motor heads and magenta on the tails, giving a🟢🟣🟢triplet. Can even see a few processive runs🏃‍♂️

[Shalin Mehta @mattersOfLight]

🔬🤖Excited to share our new preprint on robust virtual staining of key organelles! https://t.co/w8eBziTyMz

✨ Virtual staining and segmentation of nuclei and cell membranes in phase images can significantly accelerate image-based screens and developmental studies.

🔍 Current virtual staining models often struggle with nuisance variations in label-free input and don't generalize well to new cell types. We’re releasing our training protocols that lead to robust models and enable few-shot generalization.

🔧 Stay tuned for our revamped codebase with weights and example data, coming soon!

👏🧫🐟 Huge shoutout to @ZW_Liu, @edyoshikun, my team @czbiohub (Soorya, Johanna, Christian, @TalonChandler, @ieivanov1), and the teams of @LeonettiManuel, Carolina Arias, and @Adrian_Jacobo for this fun collaboration!

#VirtualStaining #DL #CellBiology #Zebrafish

[Jordan Beach @myosincity]

The search is on....

We are recruiting at the assistant professor level!
Come join us!!!

Assistant Professor job with Loyola University of Chicago - Cell and Molecular Physiology Department | 12818067 (https://t.co/Uro0poZlal)

[Srich Murugesan @srich7]

Holds true for ANY PowerPoint but especially so in biotech/pharma:

[Keith Hornberger @KRHornberger]

Can’t emphasize enough for folks who are new to crafting slide decks in pharma/biotech: less is more. Lead with key points, go to details if time permits. Convey with graphics & minimize text. If you’re in 10-12 point font, you’ve lost the plot. Leave ample time for discussion.

[Keith Hornberger @KRHornberger]

Can’t emphasize enough for folks who are new to crafting slide decks in pharma/biotech: less is more. Lead with key points, go to details if time permits. Convey with graphics & minimize text. If you’re in 10-12 point font, you’ve lost the plot. Leave ample time for discussion.

[ZEISS Microscopy @zeiss_micro]

Today, we introduce the new ZEISS Celldiscoverer 7 – your microscope with adaptable automation for advanced imaging workflows!

ZEISS Celldiscoverer 7 now features higher acquisition speed, and supports more versatile experiments and new applications. Here is what's new:

𝗛𝗶𝗴𝗵𝗲𝗿 𝗧𝗵𝗿𝗼𝘂𝗴𝗵𝗽𝘂𝘁
Enjoy up to 9 times faster widefield and up to 1.8 times faster confocal image acquisition.

𝗡𝗲𝘄 𝗟𝗶𝗴𝗵𝘁 𝗦𝗼𝘂𝗿𝗰𝗲
Shorter exposure times enable more sensitive imaging, reducing phototoxicity and bleaching.

𝗕𝗿𝗼𝗮𝗱𝗲𝗿 𝗦𝗽𝗲𝗰𝘁𝗿𝗮𝗹 𝗥𝗮𝗻𝗴𝗲
Seven excitation lines including 735 nm far red give you maximum flexibility in the choice of dyes.

𝗡𝗲𝘄 𝗖𝗮𝗺𝗲𝗿𝗮𝘀
Get higher sensitivity, signal to noise, and speed with ZEISS Axiocam 807 mono and 820 mono.

𝗔𝗱𝘃𝗮𝗻𝗰𝗲𝗱 𝗪𝗼𝗿𝗸𝗳𝗹𝗼𝘄𝘀
Full support of auto-immersion objectives significantly improves Guided Acquisition workflows.

𝗖𝘆𝗰𝗹𝗶𝗰 𝗜𝗺𝗮𝗴𝗶𝗻𝗴
Include different sample carriers in an experiment to acquire advanced time series.

𝗦𝗰𝗮𝗻𝗻𝗶𝗻𝗴 𝗔𝗿𝗲𝗮
A new sample holder maximizes the travel range for full coverage of chamber slides.

𝗦𝗵𝗮𝗱𝗶𝗻𝗴 𝗖𝗮𝗹𝗶𝗯𝗿𝗮𝘁𝗶𝗼𝗻 𝗠𝗮𝗻𝗮𝗴𝗲𝗿
Let individual users apply their custom shading corrections to different samples.

𝗗𝗮𝘁𝗮 𝗘𝘃𝗮𝗹𝘂𝗮𝘁𝗶𝗼𝗻
A new viewer introduces more options for observing and comparing data in multi-well experiments.

𝗦𝘆𝘀𝘁𝗲𝗺 𝗨𝗽𝘁𝗶𝗺𝗲
Excessive endurance testing and preventive service options ensure reliable long-term operation.

Learn more about ZEISS Celldiscoverer 7, its product features, and explore application examples: https://t.co/iQHUXICtEq

#celldiscoverer7 #hcs #hca #drugdiscovery #cellculture #imaging #microscopy

[Sreenivas Bhattiprolu @digitalsreeni]

VR isn't just for gaming. Researchers can literally step into their 3D datasets, tagging voxels for ground truth and setting the stage for advanced 3D deep learning.

#microscopy #bioimageanalysis #deeplearning

[Bertrand Delsuc @BertrandBio]

Interesting answer to the inexorable loss of momentum of🇯🇵pharma R&D

Takeda $TAK, #Astellas and #Sumitomo Mitsui Banking Announce Master Agreement to Establish Joint Venture Company for Incubation of Early Drug Discovery Programs (R&D originating from🇯🇵) https://t.co/UoqM6v91LF

[Denis Wirtz @deniswirtz]

We engineered hyper-migration into CART cells to dramatically enhance their infiltration into solid tumors.

We tested this strategy for ovarian, lung, and pancreatic cancer.

The contrast with control CAR T cells is ....you decide.

More here: https://t.co/ObfvUjKxFX

[Zebrafish Rock! @ZebrafishRock]

Tracking neuron lineages, movements, molecular identities & activity in a developing zebrafish spinal cord. Credit to Dr. Philipp Keller @HHMIJanelia. #ZebrafishZunday

[Srich Murugesan @srich7]

88% ☀️ 🌙

[Eric Betzig @Eric_Betzig]

#FluorescenceFriday #cellbiology #bioart
The interior of the cell is a busy place, as seen by grazing incidence structured illumination microscopy at 30 frames per minute:
Lysosomes (green) can become corralled by the endoplasmic reticulum (ER, magenta), but can also remodel the ER when they are actively transported at high speed along microtubules (MTs, yellow). ER tubules are also remodeled directly when they associate with the growing or shrinking ends of MTs.
https://t.co/H9kU2qeuPb