Mayuri Thete

[Sergiu P. Pasca @Sergiu_P_Pasca]

Today in @Nature, we report human sensory #assembloids—the most complex 3D assembloids to date—comprising four integrated parts to recapitulate aspects of the spinothalamic pathway that processes pain stimuli. We use this model to investigate cellular/early circuit level features of genetic pain syndromes. More details to follow.

Work pioneered in the lab by the brilliant Ji-il Kim and Kent Imaizumi, and in close collaboration with @GregScherrer25!

Link below 👇

[Xiao Yang @XiaoYang63]

Thrilled to share that I will join @JohnsHopkins @JHUBME as an Assistant Professor in 2025! The Yang Lab will develop novel bioelectronics and biomaterials for brain-machine interfaces, regenerative medicine, and the study of human neural development and diseases.

[Sergiu P. Pasca @Sergiu_P_Pasca]

Cells located in the midline of the embryo play crucial roles in development. These cells often influence cell fate decisions and guide axons to establish bilateral connections. Despite their importance, studying these transient cells and their complex interactions has been challenging.

Today, we introduce human midline #assembloids—3D self-organizing cellular clusters derived from stem cells by the functional integration of floor plate #organoids and spinal cord #organoids. We demonstrate that midline assembloids enable cell specification and axon guidance in human neurons.
Additionally, we use these assembloids to screen and identify human-enriched factors that control these processes.

Work led by the incredible @massimo_onesto & @neal_amin!!

More details in the preprint 👇

[Xiaoyu Chen @XiaoyuChenxy]

We developed a potential therapy for Timothy Syndrome. We hope one day we can reach bedside! Immensely grateful for my exceptional mentor @Sergiu_P_Pasca and co-first author @fikribirey, and contributing authors @Minyin_Li @RevahLab @RebeccaJLevyMD @TheteMayuri and everyone!

[Sergiu P. Pasca @Sergiu_P_Pasca]

Today we are sharing our most complex #assembloid to date: a 4-part model of the human ascending neural somatosensory pathway generated by functionally integrating stem cell-derived cerebral cortical, thalamic, spinal and sensory #organoids.

Check out the @biorxivpreprint and a short video of activity as these parts self-assemble

Work led by the multi-talented Ji-il Kim & Kent Imaizumi

https://t.co/MjHOBya05Q

[Mayuri Thete @TheteMayuri]

Happy to share our team’s very exciting work published on BioRXiv today!

https://t.co/HBpGEY2vpy

[Mayuri Thete @TheteMayuri]

A very proud moment for India today!!🎉🎉👏🏻

https://t.co/GOjDTRPtFt

[Kevin Kelley @kevinwkelley]

Really happy to share our preprint. The product of a truly delightful collaboration with @neal_amin , which started with a simple question:

how can we systematically generate diverse human brain cell types in a dish?

https://t.co/jalT1n9D8h

5/

[Sergiu P. Pasca @Sergiu_P_Pasca]

Latest lab group photo.
Proud of these science champions!

[Sergiu P. Pasca @Sergiu_P_Pasca]

How do we go about generating neural cell types in vitro from human pluripotent stem cells?

Generally, we leverage relevant morphogen pathways and iteratively modulate them by trial and error to generate a cell population of interest. This is tedious and we yet have to learn how to derive many disease-relevant cell types.

In an effort spearheaded by the amazing @neal_amin & @kevinwkelley we took a first-level, systematic approach to address this question by building an arrayed screen of morphogen in organoids combined with multiplexed single cell transcriptomics.

We discovered that this platform can generate considerable regional and cell type diversity across the human neural axis and it provided insights into the specification of interesting neuronal populations of the forebrain or cerebellum.

See the results in the preprint below 👇

https://t.co/CtxBzTu0iv

[Sergiu P. Pasca @Sergiu_P_Pasca]

Applications are pouring in for our @BrOrganogenesis hands-on workshop on #organoids and #assembloids
Will extend the deadline until Nov 5 as many wanted to make sure their letters are in

Instructions 👇
https://t.co/zEC7Od5zDs

Looking forward to meet this incredible cohort!

[Mayuri Thete @TheteMayuri]

@NeoPascaLab @GWISci @StanfordNeo @StanfordPeds Congrats Anca!😊🎉

[Sergiu P. Pasca @Sergiu_P_Pasca]

Teaching & implementing methods is as important as developing them
Glad to announce another international hands-on course on human models of neural development in our @Stanford @BrOrganogenesis

Come learn how to make & probe #organoids & #assembloids

Deadline Nov 1
Please RT

[Sergiu P. Pasca @Sergiu_P_Pasca]

Hundreds of genes have been associated with autism & neurodev disorders
But how do these genes impact specific stages in human development?
In a new preprint, @XianglingM took a bold approach & developed CRISPR screens in #assembloids to map 425 genes on interneuron dev stages 👇

[Sergiu P. Pasca @Sergiu_P_Pasca]

I am excited to share my @TEDTalks at #TED2022 on how lab-grown neural cells and circuits can contribute to reverse engineering human brain development & uncover the biology of neuropsychiatric disorders

https://t.co/DSms3crkYs

#assembloids #organoids #brain #Mentalhealth

[Sergiu P. Pasca @Sergiu_P_Pasca]

Our article in @CellStemCell by @fikribirey is on the cover of the new issue today. Congratulations to the whole team!

#assembloids #migration #interneurons #disease #calcium

https://t.co/89wdbnmg11

[Mayuri Thete @TheteMayuri]

Thrilled to share our teams @PascaStanford highly mechanistic study published in Cell Stem Cell today. Congratulations @fikribirey @Minyin_Li @Aaron_Gordon1 @FreddyMValencia @omerevah @NeoPascaLab !

[Fikri Birey @fikribirey]

Excited to share our study on the molecular drivers of human cortical interneuron migration in Timothy Syndrome (TS), now out in @CellStemCell! https://t.co/3W0KDKPkuL. (1/3)

[Mayuri Thete @TheteMayuri]

@NeoPascaLab 👏🏻👏🏻👏🏻

[Mayuri Thete @TheteMayuri]

Amazing! So proud!👏🏻🇮🇳

[The Olympic Games™ @Olympics]

ICONIC. #IND

[Sergiu P. Pasca @Sergiu_P_Pasca]

Collection of papers on brain #assembloids and #organoids