I'm thrilled to share our latest preprint introducing the CRISPRaTOR, a biomolecular circuit for precise gene expression control in mammalian cells, the culmination of my PhD journey here at @Tigem_Telethon. Thanks to my mentor @diegodidibi and to @FrancescoRaga12 and @Vi_Fusco
We demonstrated it through the use of a reporter Luciferase protein and nu directly tracking the rtTA fused to an EGFP. Additionally, we demonstrate this feature in both transient transfection and in a stable cell line interesting the whole circuit!
Check out our new preprint if you want to know more about new gene circuits in mammalian cells and their applications in developing whole-cell biosensors! #SynBio#MammalianCells
Since the discovery of CRISPR-Cas systems, people have wondered if a similar type of system exists in eukaryotes. Today, we report in @Nature the characterization of Fanzor, a eukaryotic RNA-guided system that can be reprogrammed for human genome editing. https://t.co/aKLMMEBRkk
#TigemDataClub
Today @AlessioMallozzi will introduce us to his new results on CRISPRsTOR: a self-regulating #CRISPRa system
More info about Diego DI Bernardo's Lab here:
https://t.co/RmGhUX0Clw
We're attending #SEED2022 Conference in Washington D.C., ready and excited to present our posters on the implementation of new genetic circuits in mammalian cells!
@GsSargenti@CorSport Ma scusa parla per te... Io commento supportando appunto ciò che dicono quelli più bravi, che non lo sono per definizione, ma perché seguono i dati. Non esprimo opinioni personali, condanno solamente l'antiscientismo.
@GsSargenti@CorSport La fama è niente senza dati. Montaigner dice stronzate da anni, appoggia omeopatia e antivaccinismo senza basi scientifiche. Il complotto sul coronavirus è solo l'ultimo dei suoi deliri. Che imbarazzo che parli di argomenti di cui non sai palesemente niente.