Ashim

PhD student at EPFL, working to combat AMR in UTIs.

[Jarrid Rector-Brooks @jarridrb]

What if AI could invent enzymes that nature hasn’t seen? 👩‍🔬🧑‍🔬

Introducing 🪩 DISCO: Diffusion for Sequence-structure CO-design

14 rounds of directed evolution and over a year of wet lab work. That's what it took to engineer an enzyme for selective C(sp³)–H insertion, one of the most challenging transformations in organic chemistry.

DISCO surpasses this with a single plate. No pre-specified catalytic residues, no template, no theozyme, no inverse folding, just joint diffusion over protein sequence and structure.

📝 Blog: https://t.co/j9Za0JigfO
📄 Paper: https://t.co/ficrYNBBrM
💻 Code: https://t.co/p81sSwoaPH

[Knut Drescher @knutdrescher]

We found that many bacterial species use exogenous peptidoglycan fragments - released by lysis of neighboring cells - as a general danger signal, triggering a danger response that protects bacteria against many dangers: biofilm formation.

Details👇:
https://t.co/qWW3bk6NBN

[NCCR AntiResist @NCCR_AntiResist]

📢In the second of today's 🦠Bug Bust 🟢 videos for #WAAW2024, the fantastic @NCCR_AntiResist PhD student @Ashim121006 @biozentrum @UniBasel_en talks about his work with polymicrobial catheter-associated UTIs.

📽️Enjoy!

#TransformingAntimicrobialResearch
@WHO @snsf_ch

[Alexander Harms @AHarms485]

Preprint alert 🚨! I present you the second and final manuscript on the BASEL phages! 36 new deeply characterized phages from viral groups that were so far mostly terra incognita - and all infect the E. coli K12 lab strain with easy tricks. 1/12
https://t.co/lqZdYvMkSG

[IISc Bangalore @iiscbangalore]

Researchers at IISc have developed a fluorescence-based technique to distinguish between antibiotic-sensitive and antibiotic-resistant pathogens, with high accuracy.

https://t.co/GAdJc50kLH
#IIScresearch

[ACS Publications Bio & Med Chem Content @ACSBioMed]

Novel probes quantify antibiotic-induced membrane damage, showing diagnostic potential against antimicrobial resistance. #ACSInfectiousDiseases

https://t.co/vDoBk92jWg

[Ashim @Ashim121006]

@TheBelsian Congratulations!

[Ashim @Ashim121006]

@UdayAdwaith Congratulations!

[Ashim @Ashim121006]

This work is the result of effort, help and guidance of everyone involved - The professors, @DipankarNandi97 , @SenSobhan and Prof. BE Pradeep, as well as the other co-authors, @sardana_deepika , @TaruVerma7 , Parvez Alam, @ImmunomanAvik , Nandini Sanil and @BalaramKhamari
(Fin)

[Ashim @Ashim121006]

After a lot of effort, and multiple rounds of improvement, this work, forming the main part of my Master’s Thesis at IISc in the lab of Prof. Dipankar Nandi @DipankarNandi97 , is finally out.
A brief thread - (1/n)

[Ashim @Ashim121006]

We developed novel interfacial probes as a diagnostic for membrane dynamics in bacteria, and discovered a new paradigm of antibiotic damage - cytoplasmic membrane thinning, in work spanning across 3 labs, over the past 2 years.
Please go over and comment.
https://t.co/47HfAkWDMd

[Ashim @Ashim121006]

All in all, we characterised a different way of looking at antibiotic resistance - to look at alterations (or lack thereof, under resistance) in the bacterial membrane upon antibiotic action, with potential for diagnostic usage.

Check it out here - https://t.co/pNA32LGfza

8/n

[EPFL Center for Imaging @EPFL_Imaging]

The EPFL Summer School in Image Analysis: 2nd Edition!

25 PhD students from across @EPFL_en
(12 doctoral programs!) are receiving a hands-on introduction to the key concepts of image analysis for their everyday research 💻🔬🔭

Great to be back in Caux 😎
#ImagingAcrossScales

[Samay Pande @iamsamayp]

1/n Happy to present the first preprint from our lab. This work was led by powerhouse of a PhD student @SaheliS95 , who was equally supported by @Bhoomika_Ashok and @jessica_laloo . Here is the thread to tell you a bit about this manuscript

https://t.co/Yd9xnQhezU

[Sean Booth @ImDrBooth]

Suicidal chemotaxis in bacteria!
Now officially published!
We found that Pseudomonas aeruginosa crawls towards lethal antibiotcs.
This was a super cool project to work on and I'm stoked to see it out!
Check out the preprint thread for details!
https://t.co/7yGfAMnVmM