Dong-Jiunn Jeffery Truong

Just some Dude. Don't take me seriously. I'm living from Memes & Science & 🥟.

[Dong-Jiunn Jeffery Truong @Earthnut89]

To make this not only work as a proof of concept but also reliable, we maximized export efficiency, ensuring the genomically integrated NTVE system works reliably without requiring high budding rates and benchmarked our system against similar systems based on exosomes or VLPs

[Dong-Jiunn Jeffery Truong @Earthnut89]

"Non-destructive transcriptomics via vesicular export" is now published in @NatureComms (https://t.co/jZoMtMcAgm), where engineered cells secrete their own transcriptome to the supernatant, enabling non-consumptive sampling!
Congrats to @niggles293T!

[Brian Searle @briansearle]

Delighted that @teerap16's paper on MAP-MS is out! https://t.co/tGpEOarO6u
It changes how precursor ions accumulate in Orbitraps to achieve 2× dynamic range without consequences, improving DDA and DIA. It's a free instrument upgrade requiring no special software for Lumos on up!

[Sternberg Lab @SternbergLab]

New pre-print(s) from the Sternberg Lab in collaboration with @LeifuChangLab! We uncover an unprecedented molecular mechanism of CRISPR-Cas12f-like proteins, which drive RNA-guided transcription independently of canonical promoter motifs.

Full story here: https://t.co/4eCvIxzGD4

[zack chiang @z_chiang]

The peer-reviewed version of expansion in situ genome sequencing is now out in Science!

The news is bittersweet – when we first revealed this last September, I never guessed it would be my final paper in academia, but a lot has changed. A few parting thoughts:

[Teeradon Phlairaharn @teerap16]

Give MAP-MS a try on your Orbitrap- no hardware or software changes needed! Curious how it works? Check out @briansearle tweet for the details. #MassSpec #Orbitrap #asms2025 Huge thanks to coauthors and everyone who supported us during this work!

[Sternberg Lab @SternbergLab]

1/10 Today in @ScienceMagazine in collaboration with @liugroup we report the development of a laboratory-evolved CRISPR-associated transposase (evoCAST) that supports therapeutically relevant levels of RNA-programmable gene insertion in human cells. https://t.co/y2DqXZzY1R

[Dong-Jiunn Jeffery Truong @Earthnut89]

@NatureBiotech Very honored to be highlighted by @NatureBiotech 😍! Thanks!

[Dong-Jiunn Jeffery Truong @Earthnut89]

ENVLPE packaging constructs are now available for ordering on @Addgene, including the most important pegRNAs, nsgRNAs and sgRNAs used in the study. https://t.co/usHqPVBZ4k

[Dong-Jiunn Jeffery Truong @Earthnut89]

https://t.co/8Cwm3nmUzS
ENVLPE, our system for VLP-based delivery of gene editors, is now published in @CellCellPress. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models.
Congrats co-first-authors @geilinho & @niggles293T!

[Helmholtz Munich | @HelmholtzMunich @HelmholtzMunich]

Engineering Smart Delivery for Gene Editors 🧬
👉https://t.co/4kiYAqoIQT

A research team from #HelmholtzMunich and @TU_Muenchen has developed an advanced delivery system that transports gene editing tools based on the CRISPR/Cas9 gene-editing system into living cells with significantly greater efficiency than before.

💡 Their technology, ENVLPE, uses engineered non-infectious virus-like particles to precisely correct defective genes – demonstrated successfully in living mouse models that are blind due to a mutation.

🌟 Precisely corrects defective genes
🌟 Promising for cancer therapy & inherited diseases
🌟 Enables creation of universal, off-the-shelf immune cells

@Earthnut89
#GeneEditing #CRISPR #CancerTherapy #Biomedicine #TranslationalResearch

[Dong-Jiunn Jeffery Truong @Earthnut89]

https://t.co/8Cwm3nmUzS
ENVLPE, our system for VLP-based delivery of gene editors, is now published in @CellCellPress. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models.
Congrats co-first-authors @geilinho & @niggles293T!

[David R. Liu @davidrliu]

Last night I was honored to receive the 2025 #BreakthroughPrize in the Life Sciences, reflecting the efforts of many students, collaborators, doctors, and patients in labs around the world. I hope this 4-minute excerpt can inspire when science needs public support more than ever.

[Feng Zhang @zhangf]

We are excited to report the discovery of TIGRs, a widely-occurring RNA-guided system found in bacteria and their viruses. TIGRs consist of a peculiar repeat region which is transcribed into RNA and processed into multiple guide RNAs to direct TIGR-associated proteins to their DNA targets. https://t.co/ketXqFVQqw

[nature @Nature]

Nature research paper: RNA control of reverse transcription in a diversity-generating retroelement

https://t.co/FRlojGDh4o

[Heiko Lickert @LickertHeiko]

Thrilled to share one of our biggest discoveries: “Inceptor binds to and directs insulin towards lysosomal degradation in beta cells”.
https://t.co/GKx6eslJz0
A tremendous collaborative effort led by @JohannaSiehler, @BilekovaSara and other members of the @LickertHeiko lab.

[Dong-Jiunn Jeffery Truong @Earthnut89]

@niggles293T @geilinho Also special thanks to @gagneurlab and @fabian_theis on advising us on the data analysis pipeline! 🙏

[Dong-Jiunn Jeffery Truong @Earthnut89]

Check out our approach on "non-destructive transcriptomics via vesicular export" (NTVE)! We have benchmarked NTVE exclusively in stably integrated cell lines, where transcript alterations by perturbations were truthfully reflected in the supernatant. ,https://t.co/17BhLA9mJp

[Dong-Jiunn Jeffery Truong @Earthnut89]

Of course, this was, as usual, a team effort, which was mainly driven by @niggles293T, @geilinho, Martin Grosshauser, et al.