Ivan Kuznetsov

Cards fellow @UCSFCardiology || Prev IM resident @PennIMResidents & MD/PhD @PennMedicine @pennbioeng || Engineered biology to study metabolic disease.

[Ivan Kuznetsov @IvanKuznet]

Led by co-first authors [@itskristinali ] & me in the [@AranyLab] at [@PennCVI] / Penn Medicine. Code + data are public (Zenodo). Preprint:
https://t.co/glqqQnoumG

[Ivan Kuznetsov @IvanKuznet]

🧵New preprint! We built a way to run CRISPR functional genomics at scale directly inside the living mouse heart: in vivo compressed AAV-Perturb-seq. 585 gene knockouts, read out one cardiomyocyte at a time.

https://t.co/glqqQnoumG

[Ivan Kuznetsov @IvanKuznet]

We also flipped it: a combination of 41 gene perturbations reconstructs the failing right-ventricle transcriptome (r ≈ 0.6). Weights learned from mouse heart failure stratified human RV failure by severity with no refitting (ρ = 0.71).

[Jay Giri @jaygirimd]

🧵 Many “hot takes” about the reduction of @NIH indirect grant costs to 15%.

Let’s lay out the facts about the university grant management process & accounting. And then use this to project likely short and longer term impacts of the policy.

[NIH @NIH]

Last year, $9B of the $35B that the National Institutes of Health (NIH) granted for research was used for administrative overhead, what is known as “indirect costs.” Today, NIH lowered the maximum indirect cost rate research institutions can charge the government to 15%, above what many major foundations allow and much lower than the 60%+ that some institutions charge the government today. This change will save more than $4B a year effective immediately.

[Ivan Kuznetsov @IvanKuznet]

Well...this is one of the coolest things I've seen in a while.

[Jeffrey Millman @JeffreyRMillman]

Big #RegenerativeMedicine advance! New study demonstrates the use of iPSC-heart muscle to treat a patient with heart failure. The patches successfully engrafted, contributing to heart repair. Patient survived 3 months before receiving a heart transplant https://t.co/3jDbvl7NKo

[Prof. Nikolai Slavov @slavov_n]

This rate of validation suggests that antibodies should be considered non-specific until proven otherwise:

357 validated out of 1,124 tested.

.

[Rong Fan @RongFan8]

See the publication link. Thanks to everyone and such an enjoyable team work!! @MinaXu7 @YiXing77 @DrMingyaoLi @JunLuLab @dingyao_zhang @MarkGerstein
https://t.co/X2FHJoIYoy

[Kazumasa Kanemaru @kazukane]

Thrilled to see our paper published @Nature.
https://t.co/kg6fSuUeI1

This is a great teamwork with @JamesCranley who is my master of human heart!
Happy to be as a member of @teichlab, and thank you to Sarah, @NosedaMichela, and everyone who is around us.

[Feng Zhang @zhangf]

Since the discovery of CRISPR-Cas systems, people have wondered if a similar type of system exists in eukaryotes. Today, we report in @Nature the characterization of Fanzor, a eukaryotic RNA-guided system that can be reprogrammed for human genome editing. https://t.co/aKLMMEBRkk

[Mikhail Shapiro (same on bsky) @mikhailshapiro]

Can you express multiple genes from a single mRNA at programmable ratios without long intervening sequences or peptide overhangs? 🤔 Yes, with SEMPER! 😉 Congrats @mtduan, @ishaanjdev and coauthors on this new tech for mammalian synbio & gene therapy. https://t.co/iJgHuNmMR2

[Vizgen @vizgen_inc]

We're excited to share a new preprint from the @AllenInstitute using #MERSCOPE to make a comprehensive high-resolution multimodal #CellAtlas of #AlzheimersDisease in #humanbrain!👏

Read the preprint to learn more: https://t.co/ynbdxtZwuw

#MERFISH #SpatialGenomics #Neurotwitter

[Alice Ting @aliceyting]

LOV-Turbo is published today in @naturemethods and plasmids for LOV-Turbo are available on Addgene, here:
https://t.co/fzZBUXfjQK

We hope many people will use LOV-Turbo in exciting new ways!
https://t.co/nl3wrGRku4

If you plan to use NanoLuc BRET to activate LOV-Turbo, please reach out, as we are engineering improved LOV-Turbo + NanoLuc variants with higher BRET efficiency.

[Joachim Goedhart @joachimgoedhart]

Finally out: mScarlet3
A brilliant and fast-maturing red fluorescent protein.
Congrats to the teams of Dorus Gadella and Antoine Royant!
https://t.co/PhyF2I8dOe

[Ali Max Erturk @erturklab]

Imagine you could stain whole adult mice with standard IgG antibodies. What you couldn't do! We present wildDISCO technology for this🤗, creating the first whole body vascular, lymphatic, and nerve atlases. By @HongchengM @jieluo692 https://t.co/c2F91KQPv4 🧵👇🏼(turn on the sound)

[Trends in Biotechnology @TrendsinBiotech]

.@pylabrobot and @chorye on work by @aliceyting on proximity labeling enabled by directed evolution: https://t.co/kw4wberKBW