Excited to share tomorrow about our work linking live imaging, CRISPR screening, and spatial transcriptomics using the 10X Xenium platform:
https://t.co/KGuhHdGtGC
You can register here: https://t.co/qdv0CcWHXd
What if we could record single-cell RNA levels and videos in the same cells? Our new VISTA-FISH assay links gene expression with neuron firing, lysosome movement, and neuron morphology. VISTA-FISH also detects sgRNAs, enabling live-cell CRISPR screens ๐งต
https://t.co/KGuhHdGtGC
The four players on the ice when the USA scored to win the Gold Medal:
Jack Hughes - Canton, MI
Zach Werenski - Grosse Pointe, MI
Dylan Larkin - Waterford, MI
Connor Hellebuyck - Commerce, MI
Hockey runs through the state of Michigan. And why Detroit will always be Hockeytown.
@_AndrewLeduc Hmm, we tried something very much like this 4 years ago but found that the protein measurements from CITE-seq were not well fit but this type of linear ODE. Our best guess is that cell surface proteins levels are too many steps downstream from translation.
What molecular functions do transposable elements perform in mammalian cells? We reviewed a flurry of recent papers about TEs to look for trends. New TE mechanisms involve pre- and post-transcriptional regulation, virus-like activity, and guided insertion.
https://t.co/NMjK7skZDU
Computational re-analysis of publicly available bulk and single-cell sequencing datasets also holds great promise for unraveling TE epigenetic and transcriptional features across organisms, tissues, cell types, developmental stages, and disease states.
This work was led by the fearless Chen Li. Check out our package on github, including notebooks to reproduce the analyses from the paper: https://t.co/2eZxhZ1miF
This paper is also a big milestone for Welch lab--our first in-house 10X multiome dataset (great work, @therealjklee)!
@therealjklee also generated a nice in-house dataset from human embryoid bodies. MultiVeloVAE revealed epigenomic/transcriptomic changes in early human differentiation (whereas MultiVelo didn't work as well).