Mark Tye

Passionate about drug discovery. Recently completed my PhD in chemical biology in the Mazitschek lab at Harvard University

[Mark Tye @Mark_A_Tye]

@mazit @NatureComms Very excited to see my manuscript published in @NatureComms about our #medicinalchemistry efforts to develop inhibitors of ProRS for #malaria. This work is supported by the development of a single step, homogeneous TR-FRET assay. Truly grateful to @Mazit and all our coauthors

[Mark Tye @Mark_A_Tye]

@mazit @NatureComms Very excited to see my manuscript published in @NatureComms about our #medicinalchemistry efforts to develop inhibitors of ProRS for #malaria. This work is supported by the development of a single step, homogeneous TR-FRET assay. Truly grateful to @Mazit and all our coauthors

[Wiley Chemistry @Wiley_Chemistry]

From Analysis & Sensing, check out this article titled "Tiny Titans: Nanobodies as Powerful Tools for TR-FRET Assay Development."

🔗: https://t.co/vMNITf7Smq

#Free access for a limited time.
@Chemist_Payne, @mazit, @HarvardCCB, @chemical_phd, @MGHCSB
@ChemEurope

[Zack Hauseman @ZHauseman]

What an amazing opportunity to be able to spearhead this project! Really enabled by the collaborative NIBR environment, a unique framework from the innovation postdoc fellowship, and top notch scientific mentorship.

[Ralph Mazitschek @mazit]

Really excited about this work. Little things can have big impacts.

[Dr. Kelvin Getley @TheGM2K]

My (admittedly very rough) attempt at a Hubble v #JWST comparison. And this is literally just the beginning.

[Mark Tye @Mark_A_Tye]

Very exciting work by @Chemist_Payne and @mazit to combine #CoraFluor and nanobodies to make TR-FRET even better. I’ve personally used this toolbox for my own unpublished work and can vouch for just how easy this is to use and that it worked very well.

[Connor Payne @Chemist_Payne]

In our latest work @AnalysisSensing, we develop a highly versatile TR-FRET assay toolbox using #CoraFluor labeled nanobodies. Pick your favorite flavor and make TR-FRET easier than ever. @mazit (1/n)

https://t.co/Wa7jHh0hOn

[🇳🇬Lọla Fagbami, PhD, MPH 😷mask💉💪🏽shot↔️space @naijaLola]

Dr. @Mark_A_Tye, y'all! #PhDone

[Mark Tye @Mark_A_Tye]

@naijaLola Thank you Lola! I appreciate you making it yesterday!

[Cell Chemical Biology @CellChemBiol]

Online now! A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader https://t.co/g0eS6a5VNa #chembiol

[Connor Payne @Chemist_Payne]

Very happy to share the final version of our manuscript in @CellChemBiol. We developed a new assay strategy to both quantify target proteins & measure TE of small molecules - all using endogenous protein in lysates from unmodified cell lines. Oh, and it only takes 1 h ⌚️✅ (1/3)

[Ralph Mazitschek @mazit]

Are you working in TPD/#PROTAC-development and would welcome a simpler, faster, higher throughput way to quantify the abundance of endogenous target proteins directly in cell lysates, while getting a sensitive target engagement assay for ligand characterization for🆓?

[ChromoTek @ChromoTekGmbH]

Congrats to @Chemist_Payne and @mazit for revealing the isoform and corepressor complex selectivity of HDAC1/2 inhibitors. Our nano-secondary alpaca anti-rabbit IgG fused to CoraFluor and Alexa Fluor® dyes was employed in TR-FRET measurements. https://t.co/XvTzTzHrEL

[Mass General Brigham Research @MGBResearchNews]

Researchers from @MGHCSB @broadinstitute and colleagues have developed we CoraFluors, a suite of novel luminescent molecules optimized for biological applications including imaging studies https://t.co/yz2dOSI9Za

[Connor Payne @Chemist_Payne]

On @biorxivpreprint we report a direct TR-FRET-based protein quantification strategy (<1 h from lysis➡️read) in unmodified cell lines. If you're into PROTACs, and you hate running Westerns, check it out! Mini-thread: (1/n)
https://t.co/GMJ92QFW4V

[Ralph Mazitschek @mazit]

This post is not for you if you a) like running Westerns or b) are not interested in targeted protein degradation. However, if you dig PROTACs and/or welcome a fast, easy and accurate way to quantify endogenous target proteins directly in lysate, keep reading. #nomorewesterns 1/n

[Ralph Mazitschek @mazit]

What if I told you that much of what we thought to know about the isoform selectivity of HDAC1/2-inhibitors is likely not true 🤔? What if told you that we have (once again) been deceived by static biochemical assays and that reality is more “complex”💡? See below a thread: 1/n

[Ella Marushchenko @Ella_Maru]

New cover from our studio! #sciart
The pace of progress in biomedical research directly depends on techniques that enable the quantitative characterization of interactions between proteins with each other or with their small molecule ligands. 1/4