Miharu MAEDA

Assistant professor, Kota Saito's Lab, Akita University in Japan.
Membrane trafficking / ER exit sites / Secretion

[Bio-protocol @bioprotocolbyte]

Detecting the Activation of Endogenous Small GTPases via Fluorescent Signals Utilizing a Split mNeonGreen: Small GTPase ActIvitY ANalyzing (SAIYAN) System:https://t.co/e1q5j0J8zS

[秋田大学【公式】 @syudaikouhou]

【プレスリリース】
細胞内分泌経路ではたらく自己制御システムの発見

↓詳細はこちらから
https://t.co/DZsanzQ4Yd

[Miharu MAEDA @MiharuMaeda]

@cell_club I’m excited to share that our research is now published in Nature Communications! Thank you to everyone involved in this work!☺️
@NatureComms #TANGO1 #ER_exit_site

[Cell Biology J-Club @cell_club]

Phosphorylation-coupled autoregulation of TANGO1 and Sec16A maintains functional ER exit sites

https://t.co/QPZkBGYa4C

[Raffaele Di Giacomo, PhD @sciqst]

Great topic! The phosphorylation-coupled autoregulation of TANGO1 and Sec16A is indeed crucial for maintaining functional ER exit sites. This dynamic process facilitates efficient protein secretion. I'm curious, do you think this mechanism might serve as a potential target for therapeutic intervention in diseases linked to ER stress or protein misfolding?

For comprehensive insights and recent biomedical studies, you might want to check out https://t.co/4Y9Imt8uIJ, a platform that can generate detailed biomedical reviews. #CellBiology #Medicine

[Nature Reviews Molecular Cell Biology @NatRevMCB]

New Online! Towards a unified framework for the function of endoplasmic reticulum exit sites https://t.co/2lajv5Z9Mt

[Miharu MAEDA @MiharuMaeda]

🚨 New finding!
How are dynamic ER exit sites (ERES) maintained? 🤔
Our new preprint reveals a phosphorylation-coupled autoregulatory system🔁 that sustains ERES function.

Now on bioRxiv: https://t.co/9Yge7wL3tx

[Journal of Cell Biology @JCellBiol]

Schematic representation of ER-Golgi interfaces observed with SAIYAN system. Miharu Maeda, Kota Saito et al. @syudaikouhou present a new #technology to detect GTPase activation in living cells https://t.co/6xEUsUQTOV
#organelles #trafficking #golgi #ER_literature

[Miharu MAEDA @MiharuMaeda]

@C_a_r_o_l_y_n Thank you Carolina!☺️

[Cell Biology J-Club @cell_club]

Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells https://t.co/Qs2gwWsBgV

[Miharu MAEDA @MiharuMaeda]

This system allows us to visualize Sar1, which binds to the ER membrane when activated, using split mNeonGreen. We demonstrated that activated Sar1 localizes to the ERGIC with Sec23 during collagen secretion, suggesting its crucial role in this process.

[Miharu MAEDA @MiharuMaeda]

We are excited to announce that our paper on the “SAIYAN” method for visualizing the activation state of small GTPases has been published today in the Journal of Cell Biology!

[Journal of Cell Biology @JCellBiol]

Miharu Maeda, Kota Saito et al. @syudaikouhou present a novel method, the Small GTPase ActIvitY ANalyzing (SAIYAN) system, for detecting the activation of endogenous small #GTPases via fluorescent signals utilizing a split mNeonGreen system. https://t.co/tgkUGfbDjm

[Ho Yi Mak @Mak_Lab_]

We are pleased to share our work on a distant TANGO1 ortholog in C. elegans. A collaboration between Jimmy Mo @oM_ymmiJ, Kwangsek Jung, Yan Li in my lab @hkust and Chao Zhai, Yonghong Yan in Meng-Qiu Dong's lab at NIBS. https://t.co/2AOJCggGsl

[Ishier Raote @ishier_raote]

Our latest paper! A promising avenue for therapeutic intervention in fibroses, characterized by excessive ECM production, via targeted inhibition of TANGO1-mediated ER export.
TANGO1 inhibitors reduce collagen secretion and limit tissue scarring
https://t.co/BRx36aVhfF

[Miharu MAEDA @MiharuMaeda]

We wrote a comment for @JCellBiol on the p24-Tango1 paper from @PastorParejaLab

[Journal of Cell Biology @JCellBiol]

Spotlight: Saito and Maeda discuss work by Yang et al. @PastorParejaLab (https://t.co/D7E0QaZtwC) showing that all four p24 subfamilies are required for secretion and their interactions with Tango1 are vital for a stable ER–Golgi interface. https://t.co/sBKPtyxXb6

[Journal of Cell Biology @JCellBiol]

p24–Tango1 interactions ensure ER-#Golgi interface stability and efficient transport, say Ke Yang, Zhi Feng, and José Carlos Pastor-Pareja @PastorParejaLa @NeuroAlc @Tsinghua_Uni: https://t.co/gehYIBg0TT

#Trafficking #Organelles #Development #Genetics ##ER_Literature

[Miharu MAEDA @MiharuMaeda]

This method can be utilized for other small GTPases! Moreover, we also reveal that the localization of activated Sar1 during collagen secretion is distinctive. Please check it out!

[Miharu MAEDA @MiharuMaeda]

This is my first post. I work on ER-Golgi trafficking at Kota Saito’s lab. We recently published a new preprint on BioRxiv. We successfully visualized the activation of Sar1 in cells using a new method. https://t.co/CI7SBUIvfH