Custom Single Cell Omics (CSCO) core facility

🧬 Transcriptomics, epigenetics, genomics and proteomics single cell technologies 🧬 Part of the Single Cell Initiative @institut_curie 🏥🇫🇷

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

We did it! Single cell ChIP-seq is now available as a service at our core facility! #singlecell #scChIPseq
@institut_curie @VallotCeline @KoningLeanne @Perielab1

[Céline Vallot @VallotCeline]

Our team is expanding, we are looking for a new collaborator - Computational Scientist (IR) - to join us at @institut_curie @CNRSbiologie @Unit3244_ICurie . Exciting single cell cancer datasets to analyse and a great opportunity for a permanent position. Postdoc, PhD or IR apply

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

After Flash-seq, FLASH-seq-ONT!!
@Vincent_Hahaut ( @IOB_ch) will be at @institut_curie tomorow, to talk about the improvement of this #scRNAseq method ! We look forward to it! #singlecell

[Mitch Guttman @mitchguttman]

Gene expression involves thousands of proteins that bind DNA, yet comprehensively mapping these is challenging. We developed ChIP-DIP – a method for simultaneous, genome-wide mapping of hundreds of DNA-protein interactions in a single experiment. https://t.co/NEH92mQTGs

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

Come and join us ! We are looking for a bioinformatics engineer who loves #SingleCell !

[Institut Curie @institut_curie]

📢 The @institut_curie Single Cell Initiative platform is looking for a motivated bioinformatics engineer to contribute to the development of single cell multi-omics technologies as part of the Curie SiRIC program ⤵️

https://t.co/mnPaL1anGZ

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

Great talk at the #SCG2023 in Engelberg! We have been amazed by all the work and advances done in #SingleCell #Genomics this past few years. Waiting for more in 2024!

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

🚨We are hiring!
The Custom Single Cell Omics (CSCO) core facility @institut_curie is looking for an engineer. If you like single cell molecular biology, platform work and technological development, come and join us! #singlecell #corefacility
https://t.co/tor6RnsBsz

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

This is promising!

[Rahul Satija @satijalab]

We are excited to introduce Phospho-seq, our approach for profiling intracellular protein and cell signaling dynamics alongside additional molecular modalities in single cells!

Check out @JBlairSci's preprint at https://t.co/LANumW2Ko4

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

Nothing could have been done without @KoningLeanne the project manager of the Single cell initiative at @institut_curie !

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

Thanks to @wamoyal & Sebastien Bellow from @vizgen_inc for this training on the #MERSCOPE! The PATHEX , NGS & Single cell facilities of @institut_curie got together with Kyra Borgman for some #spatialtranscriptomics . We can't wait to try it out on real projects!

[William Amoyal @wamoyal]

Today was the final day of the training on the the Vizgen #MERSCOPE #spatialtranscriptomics platform at Institut Curie single cell facility (Initiative Single Cell).
Thanks a lot to Mylène Bohec, Eve Moutaux , Melanie Miranda, Renaud Olivier and Kyra Bo…https://t.co/nW5YNHNp9f

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

#spatialtranscriptomic alert 🚨
The MERSCOPE @vizgen_inc arrived at our single cell facility @Institut_Curie (Initiative Single Cell). Many Curie’s teams are ready to play with it, with us and Kyra Borgman from @CoulonLab.

[Treutlein lab @TreutleinLab]

Save the date!

Single Cell Genomics 2023 #SCG2023 takes place Oct. 9-11 2023 in the beautiful mountain town Engelberg in Switzerland!

10th year anniversary of this unique experience. @IdoAmitLab, @slinnarsson, @AlexandervanOu1, @MarioniLab, @sandberglab, Aviv Regev, Amos Tanay

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

#EpigenomicMERFISH @ Xiaowei Zhuang @HarvardCCBand @Vizgen, a new method for profiling spatial epigenomic loci at sub-nuclear resolution. #singlecell #spatialepigenetic

[Vizgen @vizgen_inc]

New #MERFISH publication alert! Dr. Xiaowei Zhuang's @Harvard research team developed #EpigenomicMERFISH! Check out the open access publication available from @CellCellPress: https://t.co/wuZo8LgFUM

#Fascinating #Epigenomics #MERFISH #Brilliant

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

In detail, they identified the best tissue fixation for pA-Tn5 tagmentation: 1% PFA +HCl treatment. After tagmentation, they embedded sample in polyacrylamide gel and digested it with prot K to ensure efficient transcription. Then,MERFISH allow visualization of transcribed RNAs.

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

They are able to reveal key epigenetic marks (H3K4me3, H3K27ac,H3K27me3) of loci of interest by combining: enhanced tissue fixation, pA-Tn5 tagmentation with Mosaic End containing T7 promoter, production of RNA (amplified signal) which can then be imaged using MERFISH technology.

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

@BioRugby Maybe one day one of those multimodal chromatine technologies ;)

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

After nanoCut&Tag @GoCasteloBranco and MultiCut&Tag @ ThomasFazzio for multimodal profiling, here is the scMulTI-Tag from @mpmeers. Now it's possible to visualize up to 3 histone marks in a single experiment by coupling 2 tagmentation events followed by PCR using microfludic.

[Michael Meers @mpmeers]

Happy to report that MulTI-Tag is officially published in a special spooky 🎃 online edition of @NatureBiotech--shareable link here: https://t.co/m6VGXRI6da 1/5

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

We will be listening! FLASH-seq is a powerful improvement of Smart-seq3 ! #singlecell #scRNAseq

[Single Cell Omics Germany (SCOG) @singlecellomics]

📢 Join the SCOG Virtual Lecture #47 (Zoom)
⏰ October 21, 9:30-10:15am CEST

💬 Simone Picelli, IOB Basel (@IOB_ch)
💡 "Fast and highly sensitive full-length single-cell RNA-sequencing using FLASH-seq"

✏️ Registration: https://t.co/oGXJaO1CH4
#SCOGVLS

[Custom Single Cell Omics (CSCO) core facility @SingleCell18]

Very proud of our bioinformatician Louisa for creating CellDestiny with @Perielab1 @cosgrove47 ! 🎉

[Jason.cosgrove47 @cosgrove47]

Congrats to Louisa (@Perielab1, @SingleCell18 ) for developing CellDestiny, an app to analyse single cell lineage tracing datasets. Hopefully useful for those who want to analyse their own or published data but don't code or don't know where to start. https://t.co/OoZnd4F5Ev.