Sisi Yang

Graduate student in Cell Biology Department of Yale. Music, food, movie, travel lover

[Hanieh Falahati هانيه فلاحتى @HaniehFalahati]

Sounds super interesting!! 👀👀Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons https://t.co/AeeUJVEMUo

[Alice Ting @aliceyting]

LYTACs pioneered by @CarolynBertozzi opened the door to degradation of cell surface and extracellular proteins through lysosomal targeting. Here, we introduce genetically-encoded LYTACs, or GELYTACs, which are (1) easier to produce by recombinant expression (LYTACs, antibody conjugates to glycopeptides, are harder to make), (2) amenable to engineering via directed evolution, and (3) possible to secrete from therapeutic cells such as T-cells.

We demonstrate degradation of various soluble targets, including TGFb, IL6R ectodomain, and mCherry with GELYTACs optimized via yeast display directed evolution, added either as recombinant protein or secreted from living T-cells.

Congratulations to lead author @Jony_theChemist, our wonderful collaborators Sean Yamada-Hunter, @LouaiLabanieh and @molecular_elena from the @mackalllab, and great teaming up again with the formidable @CarolynBertozzi.

https://t.co/WVYAadruBP

[Sharon Tooze @sharontooze01]

Congratulations Alex! Another bit of the ATG9A puzzle
Exploring the ATG9A interactome uncovers interaction with VPS13A https://t.co/jba0BhIkVn

[Yale Neuroscience @YaleNeuro]

New study published in @CurrentBiology, led by first author @Sophistarete from @LabYogev
and @PDClab, reveals key to axonal transport 🪱🧠. Read more: https://t.co/MaaC1wJyjS and full article: https://t.co/CmoXuRqZvW

[Dorotea Fracchiolla_Art&Science @DoroteaArt]

🎉Excited to share with you Ayelen Valko and my recent spotlight on our latest Art&Science exhibition at the @CSHVienna now published in Autophagy 🎨🖼
➡️Read the article if you have not made it to the live event! https://t.co/BMawbKP9Gm

[Sisi Yang @SisiYang10]

@JeeyunC Thank you Jeeyun!

[Sisi Yang @SisiYang10]

The second main project from my graduate school has finally been published! In this paper we performed forward genetic screen and identified an active zone protein CLA-1 regulates sorting of autophagy protein ATG-9, which shows different phenotype with synaptic vesicle proteins.

[PLOS Biology @PLOSBiology]

#Autophagosome biogenesis occurs near presynaptic sites in #neurons. @dacolon &co reveal a role for #ActiveZone protein Clarinet in specifically regulating activity-dependent presynaptic #autophagy via sorting of the autophagy protein ATG-9 #PLOSBiology https://t.co/OTZP6G8s0x

[Daniel Colón Ramos @dacolon]

Nuevo estudio donde investigamos la relación entre autofagia y actividad neuronal. En resumidas cuentas, cuando nuestras neuronas funcionan, con uso los componentes se dañan y deben ser reciclados. Ese proceso se llama autofagia. ¿Cómo sabe la célula qué y cuándo reciclar? 1/

[Max Wilkinson @maxewilkinson]

How do LINE family retrotransposons jump around?
Can we reprogram retrotransposons?
What does this have to do with Rum and Raisin?
Answers here and below!

[Alice Ting @aliceyting]

Can we perform unbiased discovery of endogenous proteins that traffick from one organelle to another in living cells?

Learn about our new proximity labeling method, TransitID, here:

Full video of ASCB lecture at https://t.co/kEEipZNKDs

[Richard Sever @cshperspectives]

Cell biology postage stamps - new from USPS! https://t.co/Ryb1TqEdNG ht @StearnsLab

[American Society for Cell Biology @ASCBiology]

ICYMI at #cellbio2022
Shirin Bahmanyar named 2022 WICB Junior Award for Excellence in Research
https://t.co/QbMPeCTW9H

[Daniel Colón Ramos @dacolon]

Puerto Rico has received ~1.2 trillion gallons of rainfall during #Fiona. To put it in perspective, Lake Champlain in NY/VT is 6 trillion gallons of water. If the rainfall in PR during the past days were in a lake, it would be one of the largest lakes in North America.

[Denis Wirtz @deniswirtz]

We just updated our large database of 387 postdoctoral fellowships, listing description of the fellowship, $ amount, deadline, and eligibility.

Download the database here: https://t.co/EbTahdhzVP

[Andrea Cuentas-Condori 🇵🇪🔬🐛🧠 @AndreaCuentas91]

The Colón-Ramos lab is looking for research assistants
📢We welcome the international community to apply. Our community of postdocs have great mentors who are committed to your training and supporting your transition into your next professional steps.
🗓️Apply by Sep 16.

[Sisi Yang @SisiYang10]

Beautiful work of ATG9A proteomic analysis and phase study.

[Hanieh Falahati هانيه فلاحتى @HaniehFalahati]

Check out this cool beautiful work by Daehun @PDClab! 😍Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates https://t.co/TgEn37vJtr

[Aligning Science Across Parkinson’s @ASAP_Research]

ATG9 is the only core autophagy transmembrane protein present at nerve terminals, but little is known about its traffic. Grad student @SisiYang10 unraveled the mechanism of ATG9 membrane trafficking in a recent study. @PDClab @dacolon https://t.co/OHoIhwlmRB

[Zhouping Hong @ZhoupingH]

Excited to share our new story about Vps13. Here we showed the PxP motifs binding mechanisms with Vps13 VAB domain and discovered two new scramblases, MCP1 and XK for Vps13 bulk lipid transfer. https://t.co/pKiCUPyi89

[Sisi Yang @SisiYang10]

@HUST_NengWan @DoroteaArt @NeuroCellPress It depends. Mine is about $250-300.

[Sisi Yang @SisiYang10]

It was a fantastic experience working with @DoroteaArt on a ATG9 cover for @NeuroCellPress. Inspired by the Starry Night from Van Goph, we show how ATG9 vesicles traffic to forming autophagosomes with exo-endocytosis at PM through clathrin-coated pits for neuronal activity.

[Dorotea Fracchiolla_Art&Science @DoroteaArt]

It was an extreme pleasure to work with @SisiYang10 @dacolon @Yale for the design of the illustration describing their work on the role of #ATG9 in pre-synaptic autophagy, now published in @NeuroCellPress!