Nikon Imaging Center @UC San Diego

A tradition of existence that dates to 2018. Fighting entropy and the tragedy of the commons on the daily. They/Them/It.

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

Looking for live-cell imaging?

NIC@UCSD offers Nikon W1 SoRa spinning disk microscopy for fast, gentle, high-resolution imaging.

Working with cells, organoids, or tissues? We can help.

Learn more: https://t.co/vN4q0wNnQW

#CellBiology #LiveCellImaging #SpinningDiskMicroscopy

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

🔬 Need advanced microscopy support for your project?Nikon Imaging Center at UC San Diego supports academic and commercial users with confocal, spinning disk, live-cell, tissue, high-content imaging, and quantitative analysis. Reach out here: https://t.co/vN4q0wNnQW
#Microscopy

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

Yesterday was our first Microscopy Focus Group meeting! Big Thanks to presenter @JuliusBogo1 for sharing his knowledge about microscopy! It was excellent work to design and integrate machine learning processes in high throughput imaging!! @UCSD_NIC @SamReckPeterson @MelsNeurons!!

[Samara Reck-Peterson @SamReckPeterson]

This Imaging Fellows program is a unique opportunity to gain experience in advanced microscopy core facility management and to pursue a scholarly project in advanced imaging.

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

We are recruiting! https://t.co/vBrnSxp2sK

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

@rita_strack nor any stop to think

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

@christlet @MadS100tist @Maurice_Y_Lee @ManuelTHERY Totally concur. Make sure you have magnesium but not calcium in the fixation buffer, try a variety of conditions (MeOH, MeOH + PFA, PFA + Glut (with downstream NaBH4 treatments)), and be sure that your fix is mildly basic.

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

@ericvonotter I think you just described their in house QC protocol.

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

@ericvonotter Sometimes the most painful lessons stay with us longer.

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

@ericvonotter Friends don't let friends...

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

A cool paper from @barrjamie that used copious amounts of confocal imaging to examine the lung-brain connection. https://t.co/FfHlAiIC7h

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

BIG NEWS - Art of Espresso is open again!!! They are opening every day at 8am and closing sometime after lunch. Go there now and show some love!

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

In case you haven't hear about this through other channels. We're looking for a new director https://t.co/hJvohBcuZG. Come work with a great group of scientists in fabulous La Jolla, CA.

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

@MarijnSiemons @kschink @florianstroehl @JamesDManton @ASIImaging @Thorlabs This is what I was thinking of. Thanks!

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

There is an account that has been posting custom printed pieces to build a snouty light path. Could someone direct me to it?

[Wendy C. Salmon 🐟🔬🧫 @FishCsCells]

Know someone interested in a light microscopy core facility position?

The @UNC_SOM Neuro Microscopy Core is seeking an imaging specialist. Work w the amazing @cziscience imaging scientist @MichelleItano! Join the awesome imaging community @UNC !

https://t.co/haCc05kser

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

@LoogerL @joachimgoedhart @HSchihada @LABrechaun @NathanShaner No doubt. It's great for getting down to molecular mechanisms at the really small scale, but once those are answered you've got to move on.

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

What CY FRET pair do people currently recommend? mTurquoise2 + mVenusQ69M or Citrine2? @joachimgoedhart @LABrechaun @NathanShaner @LoogerL

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

@LoogerL @joachimgoedhart @HSchihada @LABrechaun @NathanShaner But you have to love that cys-light maleimide life. A former labmate tested over 600 mutants to find the right labeling pair for doing single molecule FRET on kinesin.

[Nikon Imaging Center @UC San Diego @UCSD_NIC]

@christlet @NikonInst I love looking at the traces after the fact. Sometimes you can see the training error trace suddenly drop, and it's like seeing the AI's eureka moment. We find that with a Titan RTX we can train a model in about 2:30