Manon Westra

PhD candidate | Neuroscience | Super-resolution microscopy

[Manon Westra @Westra_Manon]

Paper is out! 🎉 Working with single-molecule tracking? Have a closer look at the subdiffusive behavior in your tracks using optimized parameters and visualize temporal confinement in combination with your favorite subcellular marker! @hdmacgillavry
Check https://t.co/QhqMehoO7P

[Harold MacGillavry @hdmacgillavry]

Please spread the word for this exciting Summer School on Neural Circuit Development and Plasticity in Utrecht! We are finalising the program, but already have confirmation from amazing speakers. Soon to be updated! For now, check out: https://t.co/3swUWYALXe

[Nicky Scheefhals @NickyScheefhals]

Beyond excited to share that this paper is out! mGluR5 is transiently confined in perisynaptic nanodomains to shape synaptic function. Full article: https://t.co/dSAF2PmX7y @NatureComms @Westra_Manon @hdmacgillavry A thread🧵

[Wouter Droogers @WJDroogers]

Want to make some ORANGE CAKE? My first publication with @hdmacgillavry, Arthur de Jong and @Jelmerwillems is fully online! Duplex Labeling and Manipulation of Neuronal Proteins Using Sequential CRISPR/Cas9 Gene Editing https://t.co/F1lqtkRz1F. Here are the key messages🧵

[Harold MacGillavry @hdmacgillavry]

Online now! Team ORANGE @WJDroogers, @Jelmerwillems led by Arthur de Jong, optimised, innovated and expanded the CRISPR toolbox to co-label endogenous proteins. As a cherry on the CAKE, we apply it to acutely immobilise endogenous AMPARs at synapses! https://t.co/hqBAIxoAlU

[Jelmer Willems @Jelmerwillems]

Paper Alert!

We wrote an analysis to quantify and visualize spatial associations in single-molecule localization microscopy.

-Parameter free
-Independent of localization density
-Available as MATLAB function = easy to implement

@hdmacgillavry
https://t.co/1YJZcxxUCl

[Harold MacGillavry @hdmacgillavry]

Now out in @eLife! Dynamics and nanoscale organization of the enigmatic endocytic zone: https://t.co/a0l5xO2a1y - with new insights in activity-regulated changes in endocytic zone organization. Work from the incredible Dr @LisaCatsburg, @Westra_Manon and Annemarie van Schaijk

[Wouter Droogers @WJDroogers]

New preprint from our lab @hdmacgillavry ! Also my first preprint and first-author paper! Multiplex labeling and manipulation of endogenous neuronal proteins using sequential CRISPR/Cas9 gene editing https://t.co/GK49tgpuqV Many thanks to Artur de Jong and @Jelmerwillems Thread⬇️

[Frontiers - Neuroscience @FrontNeurosci]

New Research: Contribution of Membrane Lipids to Postsynaptic Protein Organization: The precise subsynaptic organization of proteins at the postsynaptic membrane controls synaptic transmission. In particular, postsynaptic receptor… https://t.co/TQRV2tjowc #Synaptic #Neuroscience

[Manon Westra @Westra_Manon]

Happy to share our review on the often neglected role of lipids in the postsynaptic membrane!

[Harold MacGillavry @hdmacgillavry]

@Westra_Manon and Yolanda Gutierrez wrote a review on an important, but still understudied question: how do lipids contribute to postsynaptic protein organization?https://t.co/304GfZ0ufT Happy to be part of this exciting special issue on Nano-Architecture of Neuronal Synapses!

[Manon Westra @Westra_Manon]

Let's share some 'creative science' :) Today @LisaCatsburg and I won the creativity award with our 'Wannabe a Lab Girl' song at #phdday2021 @UU_GSLS @UtrechtUni https://t.co/k76svkWqYJ

[Harold MacGillavry @hdmacgillavry]

New pre-print from the lab! Amazing work from @LisaCatsburg together with @Westra_Manon and Annemarie van Schaijk: Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses
https://t.co/rjp9aZCEsL

[Manon Westra @Westra_Manon]

@roderick_tas @NWONieuws Gefeliciteerd!!

[Harold MacGillavry @hdmacgillavry]

Preprint! In this exciting study spearheaded by @bodzeta with key contributions from @DrFlorianBerger, we present surprising new findings on how the dynamic nanoscale positioning of presynaptic mGluRs is regulated and contributes to synaptic signaling. https://t.co/xnVZ5B8aDG

[Harold MacGillavry @hdmacgillavry]

Our paper describing ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons, is out now in #PLOSBiology! https://t.co/KTuRljbww9
Fantastic work by @Jelmerwillems and Arthur de Jong in the lab, many thanks to all who contributed!

[Nicky Scheefhals @NickyScheefhals]

Our paper is out!!🎉 Shank proteins anchor the endocytic zone to the postsynaptic density to govern efficient trafficking and signaling of mGluR5 at synapses to modulate neuronal functioning. Full article: https://t.co/9xWfJGlgsb @CellReports @hdmacgillavry @LisaCatsburg

[Faculty of Science, Utrecht University @UUBeta]

Utrechtse neurowetenschappers hebben de werking opgehelderd van eiwitten die een rol spelen in de signaaloverdracht tussen neuronen: https://t.co/wHSYYWMJsy @hdmacgillavry @NickyScheefhals @UniUtrecht

[NWO Domain Science (Inactive) @NWO_Science]

#physics #lifesciences | @Jelmerwillems presents two combined hot topics, super resolution imaging and CRISPR/Cas9, into one study. @UniUtrecht
Arthur de Jong, @NickyScheefhals, @hdmacgillavry

Join the parallel session on Mon 7 oct, 15.00 am

#DBP19 #NeuronsandSensing

[Harold MacGillavry @hdmacgillavry]

Want to try ORANGE? Our knock-in constructs to tag endogenous proteins in neurons with CRISPR/Cas9-mediated genome editing are now available on AddGene: https://t.co/7Gmv1GXCqN
Tag your favorite cytoskeletal proteins, signaling molecules, synaptic scaffolds, channels, receptors!

[Harold MacGillavry @hdmacgillavry]

Just a Friday afternoon in the lab. Look at these incredible synapses! Live-cell, single-molecule localization (PALM) acquisition by @Westra_Manon #superresolution #Nanoimager @oniHQ